| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra035049 |
| Family | GT20 |
| Protein Properties | Length: 951 Molecular Weight: 106943 Isoelectric Point: 7.0998 |
| Chromosome | Chromosome/Scaffold: 07 Start: 21946578 End: 21951100 |
| Description | trehalose-6-phosphate synthase |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT20 | 88 | 556 | 0 |
| NRQRLLVVANRLPVSAVRRGEDSWSLEISAGGLVSALLGVKEFEARWIGWAGVNVPDEVGQKALTKALAEKRCIPVFLDEEIVHQYYNGYCNNILWPLFH YLGLPQEDRLATTRSFQSQFTAYKKANQMFADVVNEHYEEGDVVWCHDYHLMFLPKCLKEYNSKMKVGWFLHTPFPSSEIHRTLPSRSELLRSVLAADLV GFHTYDYARHFVSACTRILGLEGTPEGVEDQGRLTRVAAFPIGIDSERFIRALEVPDVIQHMKELKERFAGRKVMLGVDRLDMIKGIPQKILAFEKFLEE NASWRDKVVLLQIAVPTRTDVSEYQKLTSQVHEIVGRINGRFGTLTQVPIHHLDRSLDFYALCALYAVTDVALVTSLRDGMNLVSYEFVACQEAKKGVLI LSEFAGAAQSLGAGAILVNPWNITEVAASIGQALNMSPEEREKRHRHNFHHVKTHTAQEWAETFVSELN | |||
| Full Sequence |
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| Protein Sequence Length: 951 Download |
| MPGNKYNLSS SHIPLSRTER LLRERELREK RRSNRALNPN DNPDNSENDL RLEGDSSRQQ 60 YVEQYLEGAA AAMAHDDVCE RQEVRPYNRQ RLLVVANRLP VSAVRRGEDS WSLEISAGGL 120 VSALLGVKEF EARWIGWAGV NVPDEVGQKA LTKALAEKRC IPVFLDEEIV HQYYNGYCNN 180 ILWPLFHYLG LPQEDRLATT RSFQSQFTAY KKANQMFADV VNEHYEEGDV VWCHDYHLMF 240 LPKCLKEYNS KMKVGWFLHT PFPSSEIHRT LPSRSELLRS VLAADLVGFH TYDYARHFVS 300 ACTRILGLEG TPEGVEDQGR LTRVAAFPIG IDSERFIRAL EVPDVIQHMK ELKERFAGRK 360 VMLGVDRLDM IKGIPQKILA FEKFLEENAS WRDKVVLLQI AVPTRTDVSE YQKLTSQVHE 420 IVGRINGRFG TLTQVPIHHL DRSLDFYALC ALYAVTDVAL VTSLRDGMNL VSYEFVACQE 480 AKKGVLILSE FAGAAQSLGA GAILVNPWNI TEVAASIGQA LNMSPEEREK RHRHNFHHVK 540 THTAQEWAET FVSELNDTVI EAQLRISKVP PELPQDDAIR RYSKSTNRLL ILGFNATLTE 600 PVDNQGRRGD QIKEMDLNLH PELKGPLKAL CSDPSTTIVV LSGSSRSVLD KNFGEYDMWL 660 AAENGMFLRL TNGEWMTTMP EHLNMEWVDS VKHVFKYFTE RTPRSHFETR DTSLIWNYKY 720 ADIEFGRLQA RDLLQHLWTG PISNASVDVV QGSRSVEVRA VGVTKGAAID RILGEIVHSK 780 SMTTPIDYVL CIGHFLGKDE DVYTFFEPEL PSDIIPAISR SRPSSDSGGT RSSSGGGDRR 840 PPSKSTHHNN NNSKSSSKSS SSNSNHHNNK ASSQRSLQTA KSGSNNHSLG NSRRPSPEKI 900 SWNVLDLKGE NYFSCAVGRT RTSARYLLGS PDDVVCFLEK LADTSAPNSS * 960 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN03063 | PLN03063 | 7.0e-13 | 884 | 942 | 59 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| PLN03064 | PLN03064 | 5.0e-26 | 874 | 948 | 77 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| PLN03064 | PLN03064 | 0 | 1 | 810 | 814 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| PLN03063 | PLN03063 | 0 | 73 | 810 | 738 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| TIGR02400 | trehalose_OtsA | 0 | 91 | 557 | 469 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming]. This enzyme catalyzes the key, penultimate step in biosynthesis of trehalose, a compatible solute made as an osmoprotectant in some species in all three domains of life. The gene symbol OtsA stands for osmotically regulated trehalose synthesis A. Trehalose helps protect against both osmotic and thermal stresses, and is made from two glucose subunits. This model excludes glucosylglycerol-phosphate synthase, an enzyme of an analogous osmoprotectant system in many cyanobacterial strains. This model does not identify archaeal examples, as they are more divergent than glucosylglycerol-phosphate synthase. Sequences that score in the gray zone between the trusted and noise cutoffs include a number of yeast multidomain proteins in which the N-terminal domain may be functionally equivalent to this family. The gray zone also includes the OtsA of Cornyebacterium glutamicum (and related species), shown to be responsible for synthesis of only trace amounts of trehalose while the majority is synthesized by the TreYZ pathway; the significance of OtsA in this species is unclear (see Wolf, et al., PMID:12890033) [Cellular processes, Adaptations to atypical conditions]. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0005992 | trehalose biosynthetic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CAA69879.1 | 0 | 1 | 946 | 1 | 940 | trehalose-6-phosphate synthase [Arabidopsis thaliana] |
| RefSeq | NP_177979.1 | 0 | 1 | 946 | 1 | 940 | ATTPS1 (TREHALOSE-6-PHOSPHATE SYNTHASE); alpha,alpha-trehalose-phosphate synthase (UDP-forming)/ transferase, transferring glycosyl groups [Arabidopsis thaliana] |
| RefSeq | XP_002285632.1 | 0 | 1 | 942 | 1 | 921 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002331300.1 | 0 | 1 | 945 | 1 | 921 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002531237.1 | 0 | 1 | 946 | 1 | 924 | trehalose-6-phosphate synthase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2wtx_D | 0 | 91 | 559 | 3 | 455 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_C | 0 | 91 | 559 | 3 | 455 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_B | 0 | 91 | 559 | 3 | 455 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_A | 0 | 91 | 559 | 3 | 455 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 1gz5_D | 0 | 91 | 559 | 2 | 454 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| trehalose biosynthesis I | TREHALOSE6PSYN-RXN | EC-2.4.1.15 | α,α-trehalose-phosphate synthase (UDP-forming) |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| ES813375 | 359 | 452 | 810 | 0 |
| CX546763 | 315 | 66 | 380 | 0 |
| CV711307 | 294 | 88 | 381 | 0 |
| DW069910 | 288 | 177 | 464 | 0 |
| DK458019 | 266 | 388 | 653 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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