| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra035064 |
| Family | GT4 |
| Protein Properties | Length: 407 Molecular Weight: 45275.3 Isoelectric Point: 7.9083 |
| Chromosome | Chromosome/Scaffold: 07 Start: 22002223 End: 22003922 |
| Description | UDP-Glycosyltransferase superfamily protein |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT4 | 214 | 372 | 8.1976e-43 |
| FLSINRFEKKKNIDLAVSAFAILCKHKLTLSDDVTLTVAGGYDERLKENVEYLEELKSLAEKEGVSNRVNFITSCSTAERNELLSSCLCVLYTPTDEHFG IVPLEAMAAYKPVIACNSGGPVETVKSGATGYLCEPSPEDFSSAMAKFVEDPELARRMG | |||
| Full Sequence |
|---|
| Protein Sequence Length: 407 Download |
| MAKRKEGSKK MNIAIIHPDL GIGGAERLIV DAAVELASQG HKVHVFTSHH DKSRCFEETL 60 SGIFQVTVYG SFLPRHIFYR LHAVCAYLRC LFVALCVLLG WSSFDVVLAD QVSVVVPLLK 120 LKRSSKVVFY CHFPDLLLAK HTTALRRMYR KPIDFLEEQT TGMADMILVN SNFTASTFAK 180 TFKRLHGRGS RPAVLYPAVN IDQFIEPHTY KLNFLSINRF EKKKNIDLAV SAFAILCKHK 240 LTLSDDVTLT VAGGYDERLK ENVEYLEELK SLAEKEGVSN RVNFITSCST AERNELLSSC 300 LCVLYTPTDE HFGIVPLEAM AAYKPVIACN SGGPVETVKS GATGYLCEPS PEDFSSAMAK 360 FVEDPELARR MGSEARKHVV ESFSAKTFGE KLNQYLVDVV SSPKED* 420 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| pfam00534 | Glycos_transf_1 | 5.0e-37 | 214 | 378 | 166 | + Glycosyl transferases group 1. Mutations in this domain of human PIGA lead to disease (Paroxysmal Nocturnal haemoglobinuria). Members of this family transfer activated sugars to a variety of substrates, including glycogen, Fructose-6-phosphate and lipopolysaccharides. Members of this family transfer UDP, ADP, GDP or CMP linked sugars. The eukaryotic glycogen synthases may be distant members of this family. | ||
| cd03798 | GT1_wlbH_like | 4.0e-40 | 13 | 392 | 397 | + This family is most closely related to the GT1 family of glycosyltransferases. wlbH in Bordetella parapertussis has been shown to be required for the biosynthesis of a trisaccharide that, when attached to the B. pertussis lipopolysaccharide (LPS) core (band B), generates band A LPS. | ||
| COG0438 | RfaG | 7.0e-43 | 9 | 401 | 402 | + Glycosyltransferase [Cell envelope biogenesis, outer membrane] | ||
| cd03801 | GT1_YqgM_like | 9.0e-54 | 12 | 392 | 396 | + This family is most closely related to the GT1 family of glycosyltransferases and named after YqgM in Bacillus licheniformis about which little is known. Glycosyltransferases catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. The acceptor molecule can be a lipid, a protein, a heterocyclic compound, or another carbohydrate residue. This group of glycosyltransferases is most closely related to the previously defined glycosyltransferase family 1 (GT1). The members of this family may transfer UDP, ADP, GDP, or CMP linked sugars. The diverse enzymatic activities among members of this family reflect a wide range of biological functions. The protein structure available for this family has the GTB topology, one of the two protein topologies observed for nucleotide-sugar-dependent glycosyltransferases. GTB proteins have distinct N- and C- terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. The members of this family are found mainly in certain bacteria and archaea. | ||
| cd03805 | GT1_ALG2_like | 0 | 11 | 392 | 395 | + This family is most closely related to the GT1 family of glycosyltransferases. ALG2, a 1,3-mannosyltransferase, in yeast catalyzes the mannosylation of Man(2)GlcNAc(2)-dolichol diphosphate and Man(1)GlcNAc(2)-dolichol diphosphate to form Man(3)GlcNAc(2)-dolichol diphosphate. A deficiency of this enzyme causes an abnormal accumulation of Man1GlcNAc2-PP-dolichol and Man2GlcNAc2-PP-dolichol, which is associated with a type of congenital disorders of glycosylation (CDG), designated CDG-Ii, in humans. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0009058 | biosynthetic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAC83030.1 | 0 | 1 | 406 | 1 | 405 | Similar to gene pi010 glycosyltransferase gi |
| GenBank | AAM26674.1 | 0 | 1 | 406 | 1 | 403 | At1g78800/F9K20_16 [Arabidopsis thaliana] |
| GenBank | ACU21459.1 | 0 | 5 | 406 | 3 | 407 | unknown [Glycine max] |
| RefSeq | NP_178001.2 | 0 | 1 | 406 | 1 | 403 | glycosyl transferase family 1 protein [Arabidopsis thaliana] |
| RefSeq | XP_002525620.1 | 0 | 5 | 406 | 3 | 408 | alpha-1,3-mannosyltransferase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2f9f_A | 7e-17 | 214 | 366 | 26 | 164 | A Chain A, Crystal Structure Of The Putative Mannosyl Transferase (Wbaz-1)from Archaeoglobus Fulgidus, Northeast Structural Genomics Target Gr29a |
| PDB | 2jjm_L | 0.000000000007 | 214 | 404 | 214 | 390 | A Chain A, Crystal Structure Of A Family Gt4 Glycosyltransferase From Bacillus Anthracis Orf Ba1558. |
| PDB | 2jjm_K | 0.000000000007 | 214 | 404 | 214 | 390 | A Chain A, Crystal Structure Of A Family Gt4 Glycosyltransferase From Bacillus Anthracis Orf Ba1558. |
| PDB | 2jjm_J | 0.000000000007 | 214 | 404 | 214 | 390 | A Chain A, Crystal Structure Of A Family Gt4 Glycosyltransferase From Bacillus Anthracis Orf Ba1558. |
| PDB | 2jjm_I | 0.000000000007 | 214 | 404 | 214 | 390 | A Chain A, Crystal Structure Of A Family Gt4 Glycosyltransferase From Bacillus Anthracis Orf Ba1558. |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| dolichyl-diphosphooligosaccharide biosynthesis | RXN-5462 | EC-2.4.1.132 | GDP-Man:Man1GlcNAc2-PP-Dol α-1,3-mannosyltransferase |
| dolichyl-diphosphooligosaccharide biosynthesis | RXN-5463 | EC-2.4.1.257 | GDP-Man:Man2GlcNAc2-PP-Dol α-1,6-mannosyltransferase |
