| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra035295 |
| Family | CE16 |
| Protein Properties | Length: 289 Molecular Weight: 32488.2 Isoelectric Point: 10.0894 |
| Chromosome | Chromosome/Scaffold: 000103 Start: 42815 End: 47409 |
| Description | SGNH hydrolase-type esterase superfamily protein |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| CE16 | 47 | 276 | 1.5e-27 |
| GINFASGGSGYDPLTAKIVRVVSLEDQLKYFQEYKEKIKAIVGEEKAKFMVENSLYLVVASSNDLAHTYIARSLRYNSTEYPDYLADMSSKFVKKLYGLG ARRIAVFSAVPVGCLPSRRTLNGVKRKCSENLNKMALQFNAKLSPALVALRKKSPGSKIVLVDVYDILHNMIENPKKYGFEVANRGCCGTGLFEVLILCN KINPFTCKNASSYVFWDSYHPTEKAYQVIV | |||
| Full Sequence |
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| Protein Sequence Length: 289 Download |
| MIVKLIRNSV LFYFRRFKRI QPERLGIAKT IPAYLDPALK GKDLLKGINF ASGGSGYDPL 60 TAKIVRVVSL EDQLKYFQEY KEKIKAIVGE EKAKFMVENS LYLVVASSND LAHTYIARSL 120 RYNSTEYPDY LADMSSKFVK KLYGLGARRI AVFSAVPVGC LPSRRTLNGV KRKCSENLNK 180 MALQFNAKLS PALVALRKKS PGSKIVLVDV YDILHNMIEN PKKYGFEVAN RGCCGTGLFE 240 VLILCNKINP FTCKNASSYV FWDSYHPTEK AYQVIVDKLL GIYIPQLV* 300 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| pfam00657 | Lipase_GDSL | 7.0e-13 | 99 | 279 | 183 | + GDSL-like Lipase/Acylhydrolase. | ||
| cd01847 | Triacylglycerol_lipase_like | 6.0e-13 | 99 | 282 | 190 | + Triacylglycerol lipase-like subfamily of the SGNH hydrolases, a diverse family of lipases and esterases. The tertiary fold of the enzyme is substantially different from that of the alpha/beta hydrolase family and unique among all known hydrolases; its active site closely resembles the Ser-His-Asp(Glu) triad found in other serine hydrolases. Members of this subfamily might hydrolyze triacylglycerol into diacylglycerol and fatty acid anions. | ||
| cd01846 | fatty_acyltransferase_like | 2.0e-28 | 44 | 280 | 242 | + Fatty acyltransferase-like subfamily of the SGNH hydrolases, a diverse family of lipases and esterases. The tertiary fold of the enzyme is substantially different from that of the alpha/beta hydrolase family and unique among all known hydrolases; its active site closely resembles the Ser-His-Asp(Glu) triad found in other serine hydrolases. Might catalyze fatty acid transfer between phosphatidylcholine and sterols. | ||
| PLN03156 | PLN03156 | 1.0e-88 | 19 | 280 | 270 | + GDSL esterase/lipase; Provisional | ||
| cd01837 | SGNH_plant_lipase_like | 3.0e-109 | 23 | 280 | 260 | + SGNH_plant_lipase_like, a plant specific subfamily of the SGNH-family of hydrolases, a diverse family of lipases and esterases. The tertiary fold of the enzyme is substantially different from that of the alpha/beta hydrolase family and unique among all known hydrolases; its active site closely resembles the Ser-His-Asp(Glu) triad found in other serine hydrolases. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0006629 | lipid metabolic process |
| GO:0016788 | hydrolase activity, acting on ester bonds |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| DDBJ | BAB08450.1 | 0 | 20 | 288 | 50 | 319 | GDSL-motif lipase/hydrolase-like protein [Arabidopsis thaliana] |
| DDBJ | BAG68919.1 | 0 | 23 | 287 | 45 | 310 | carboxylic ester hydrolase [Arabidopsis thaliana] |
| RefSeq | NP_199032.2 | 0 | 20 | 288 | 100 | 369 | carboxylesterase/ hydrolase, acting on ester bonds [Arabidopsis thaliana] |
| RefSeq | NP_564738.2 | 0 | 23 | 287 | 45 | 310 | carboxylesterase/ hydrolase, acting on ester bonds / lipase [Arabidopsis thaliana] |
| RefSeq | NP_564741.3 | 0 | 23 | 287 | 83 | 348 | GDSL-motif lipase, putative [Arabidopsis thaliana] |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| triacylglycerol degradation | TRIACYLGLYCEROL-LIPASE-RXN | EC-3.1.1.3 | triacylglycerol lipase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| DK541011 | 240 | 50 | 289 | 0 |
| GR439533 | 224 | 66 | 289 | 0 |
| DK561425 | 208 | 23 | 230 | 0 |
| DK542975 | 206 | 23 | 228 | 0 |
| DK557676 | 206 | 23 | 228 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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