| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra035782 |
| Family | GH13 |
| Protein Properties | Length: 897 Molecular Weight: 103077 Isoelectric Point: 6.2571 |
| Chromosome | Chromosome/Scaffold: 05 Start: 17170804 End: 17176200 |
| Description | Alpha amylase family protein |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH13 | 422 | 740 | 3.6e-23 |
| TKKVLPHVKRAGYNAIQLIGIPEHKDYYTVGYRVTNFFAVSSRYGTPDDFKRLVDEAHGLGLLVFLDIVQSYAAADQMVGLSLFDGSNDCYFHHGKRGHH KHWGTRMFKYGDLDVFHFLISNLNWWITEYQVDGYQFHSLASMIYTHNGFAPFNSGFDDYCNQYVDRDALMYLILANEILHDLHPDIITIAEDATYYPGL CEPVSQGGLGFDYYVNLSATEMWVSLLDSVPDSEWSMSKIVSTLVANKEYADKMLSYAESHNQSISGGRSFAEILFGGIQNEIKLLDRGVSLHKMIRLLT FTIGGRAYLNFMGNEFGHP | |||
| Full Sequence |
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| Protein Sequence Length: 897 Download |
| MVSLSNQTSF SFYPNNLFVS DKTRLGIPGI NFPRKIKVKV TCFAADQPRQ QKQQKKKKSQ 60 STNSDADSGV DPVGFLTKLG IADRIFAQFL RERHKAIKDL KDELLKRHFD LRDLASGFEL 120 LGMHRHMEHR VDFIDWGPGA RYGAIIGDFN GWSPTENSAR EGLFGHDDFG YWFIILEDKL 180 REGEEPEELY FQQYNYVDDY DKGDSGVTAE EVFQRANDEY WEPGEDRFIK NRYEVPAKLY 240 EQLFGPNSPQ TLEDLGEIPD AETRYKQYKE EHKNDPPSNL PPCDIIDDGR GKPYDIYNVV 300 TSPEWTKKFY EKSPPIPYWL ETRKGRKAWL EKYIPAVPHG SKYRLYFNTP DGPLERVPAW 360 ATYVQPEDEG KQAYAIHWEP SPESAYKWKH SKPDKPKSLR IYECHVGISG SEAKISSFED 420 FTKKVLPHVK RAGYNAIQLI GIPEHKDYYT VGYRVTNFFA VSSRYGTPDD FKRLVDEAHG 480 LGLLVFLDIV QSYAAADQMV GLSLFDGSND CYFHHGKRGH HKHWGTRMFK YGDLDVFHFL 540 ISNLNWWITE YQVDGYQFHS LASMIYTHNG FAPFNSGFDD YCNQYVDRDA LMYLILANEI 600 LHDLHPDIIT IAEDATYYPG LCEPVSQGGL GFDYYVNLSA TEMWVSLLDS VPDSEWSMSK 660 IVSTLVANKE YADKMLSYAE SHNQSISGGR SFAEILFGGI QNEIKLLDRG VSLHKMIRLL 720 TFTIGGRAYL NFMGNEFGHP ERVEFPTQSN KFSFSLANRR WDLLESGIHH QLFSFDKDLM 780 DLDKSKGILS RGLPSIHHVN DANMVISFSR GPFLFIFNFH PSNSYEKYGV GVEEAGEYTM 840 ILNSDEAKYG GQGLLKEDQY LQRSISKRID GQRNCLEVFL PSRTAQVYKL TRILRI* 900 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN02447 | PLN02447 | 2.0e-9 | 86 | 178 | 93 | + 1,4-alpha-glucan-branching enzyme | ||
| cd11321 | AmyAc_bac_euk_BE | 1.0e-167 | 382 | 777 | 407 | + Alpha amylase catalytic domain found in bacterial and eukaryotic branching enzymes. Branching enzymes (BEs) catalyze the formation of alpha-1,6 branch points in either glycogen or starch by cleavage of the alpha-1,4 glucosidic linkage yielding a non-reducing end oligosaccharide chain, and subsequent attachment to the alpha-1,6 position. By increasing the number of non-reducing ends, glycogen is more reactive to synthesis and digestion as well as being more soluble. This group includes bacterial and eukaryotic proteins. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
| PLN02960 | PLN02960 | 0 | 1 | 896 | 900 | + alpha-amylase | ||
| PLN03244 | PLN03244 | 0 | 1 | 896 | 903 | + alpha-amylase; Provisional | ||
| PLN02447 | PLN02447 | 0 | 335 | 888 | 568 | + 1,4-alpha-glucan-branching enzyme | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0005975 | carbohydrate metabolic process |
| GO:0043169 | cation binding |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| DDBJ | BAB02827.1 | 0 | 1 | 896 | 1 | 903 | starch-branching enzyme-like protein [Arabidopsis thaliana] |
| EMBL | CBI26672.1 | 0 | 1 | 896 | 1 | 896 | unnamed protein product [Vitis vinifera] |
| RefSeq | NP_001154629.1 | 0 | 1 | 896 | 1 | 899 | alpha-amylase/ catalytic/ cation binding [Arabidopsis thaliana] |
| RefSeq | NP_188679.2 | 0 | 1 | 896 | 1 | 869 | alpha-amylase/ catalytic/ cation binding [Arabidopsis thaliana] |
| RefSeq | XP_002529457.1 | 0 | 4 | 887 | 5 | 892 | 1,4-alpha-glucan branching enzyme, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3amk_A | 0 | 335 | 888 | 114 | 690 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
| PDB | 3vu2_B | 0 | 335 | 888 | 114 | 690 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
| PDB | 3vu2_A | 0 | 335 | 888 | 114 | 690 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| starch biosynthesis | RXN-7710 | EC-2.4.1.18 | 1,4-α-glucan branching enzyme |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| DK500153 | 271 | 1 | 271 | 0 |
| DK499782 | 266 | 1 | 266 | 0 |
| DK503014 | 249 | 1 | 249 | 0 |
| EE529675 | 238 | 367 | 604 | 0 |
| BF272517 | 278 | 356 | 633 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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