| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra035904 |
| Family | CE10 |
| Protein Properties | Length: 329 Molecular Weight: 35697.4 Isoelectric Point: 5.2221 |
| Chromosome | Chromosome/Scaffold: 09 Start: 2992584 End: 2993570 |
| Description | carboxyesterase 20 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| CE10 | 35 | 311 | 0 |
| PATPDPSPQTPVVSKDISINKSKSTWMRLYAPSSTLNDAVPSQKLPLVVYYHGGGFVLCSVDLQVFHGFCSDMARDLNAIVASPSYRLAPEHRLPAAYDD GAEALEWIRNSDEEWIKSRADLSNVFLMGTSAGGNLAYNVGMRSSAAASDLSPLRIRGMILHHPFFGGEERSGSEIRLANDQVCPPIVSDVFWDLSLPVG VDRDHEYSNPTVGEGSGGLEKIGGLRWKVMVVGGEEDPMVDRQRDVAKLMKRKGVEVVEVFNDGDVHGAEIGDPSKR | |||
| Full Sequence |
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| Protein Sequence Length: 329 Download |
| MSEPTPISDP YAYLNIVKNP DGSITRDLTK FPCTPATPDP SPQTPVVSKD ISINKSKSTW 60 MRLYAPSSTL NDAVPSQKLP LVVYYHGGGF VLCSVDLQVF HGFCSDMARD LNAIVASPSY 120 RLAPEHRLPA AYDDGAEALE WIRNSDEEWI KSRADLSNVF LMGTSAGGNL AYNVGMRSSA 180 AASDLSPLRI RGMILHHPFF GGEERSGSEI RLANDQVCPP IVSDVFWDLS LPVGVDRDHE 240 YSNPTVGEGS GGLEKIGGLR WKVMVVGGEE DPMVDRQRDV AKLMKRKGVE VVEVFNDGDV 300 HGAEIGDPSK RKPLFASIGK FISSFAAL* 360 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| pfam00135 | COesterase | 2.0e-8 | 69 | 168 | 116 | + Carboxylesterase family. | ||
| cd00312 | Esterase_lipase | 1.0e-8 | 69 | 188 | 135 | + Esterases and lipases (includes fungal lipases, cholinesterases, etc.) These enzymes act on carboxylic esters (EC: 3.1.1.-). The catalytic apparatus involves three residues (catalytic triad): a serine, a glutamate or aspartate and a histidine.These catalytic residues are responsible for the nucleophilic attack on the carbonyl carbon atom of the ester bond. In contrast with other alpha/beta hydrolase fold family members, p-nitrobenzyl esterase and acetylcholine esterase have a Glu instead of Asp at the active site carboxylate. | ||
| COG2272 | PnbA | 1.0e-9 | 26 | 168 | 157 | + Carboxylesterase type B [Lipid metabolism] | ||
| COG0657 | Aes | 2.0e-32 | 61 | 327 | 267 | + Esterase/lipase [Lipid metabolism] | ||
| pfam07859 | Abhydrolase_3 | 5.0e-60 | 82 | 302 | 221 | + alpha/beta hydrolase fold. This catalytic domain is found in a very wide range of enzymes. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0008152 | metabolic process |
| GO:0016787 | hydrolase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ABB89014.1 | 0 | 13 | 312 | 2 | 301 | CXE carboxylesterase [Actinidia arguta] |
| RefSeq | NP_201024.1 | 0 | 1 | 324 | 1 | 325 | AtCXE20 (Arabidopsis thaliana carboxyesterase 20); carboxylesterase |
| RefSeq | XP_002267455.1 | 0 | 13 | 323 | 13 | 326 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002271453.1 | 0 | 16 | 324 | 1 | 312 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002322442.1 | 0 | 2 | 322 | 8 | 327 | predicted protein [Populus trichocarpa] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2o7v_A | 0 | 3 | 322 | 7 | 327 | A Chain A, Golgi Alpha-mannosidase Ii In Complex With Kifunensine |
| PDB | 2o7r_A | 0 | 3 | 322 | 7 | 327 | A Chain A, Plant Carboxylesterase Aecxe1 From Actinidia Eriantha With Acyl Adduct |
| PDB | 2zsi_A | 9e-38 | 7 | 291 | 31 | 316 | A Chain A, Plant Carboxylesterase Aecxe1 From Actinidia Eriantha With Acyl Adduct |
| PDB | 2zsh_A | 9e-38 | 7 | 291 | 31 | 316 | B Chain B, Structural Basis Of Gibberellin(Ga3)-Induced Della Recognition By The Gibberellin Receptor |
| PDB | 3ed1_F | 7e-36 | 7 | 296 | 23 | 322 | B Chain B, Structural Basis Of Gibberellin(Ga3)-Induced Della Recognition By The Gibberellin Receptor |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| formononetin biosynthesis | RXN-3284 | EC-4.2.1.105 | 2-hydroxyisoflavanone dehydratase |
| formononetin biosynthesis | RXN-3625 | - | 2,7-dihydroxy-4'-methoxyisoflavanone dehydratase |
| isoflavonoid biosynthesis I | RXN-3284 | EC-4.2.1.105 | 2-hydroxyisoflavanone dehydratase |
| isoflavonoid biosynthesis II | RXN-3303 | EC-4.2.1.105 | 2-hydroxyisoflavanone dehydratase |
| isoflavonoid biosynthesis II | RXN-5502 | - | 2,7,5-trihydroxy-4'-methoxyisoflavanone dehydratase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| DK500897 | 286 | 43 | 328 | 0 |
| JZ152128 | 312 | 10 | 321 | 0 |
| CX192632 | 246 | 1 | 246 | 0 |
| EV044856 | 240 | 1 | 240 | 0 |
| DK459515 | 252 | 70 | 321 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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