| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra036652 |
| Family | GH32 |
| Protein Properties | Length: 599 Molecular Weight: 67232.1 Isoelectric Point: 6.6888 |
| Chromosome | Chromosome/Scaffold: 09 Start: 5860107 End: 5863772 |
| Description | Glycosyl hydrolases family 32 protein |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH32 | 107 | 289 | 0 |
| HFQPEKNWMNGPLFYKGLYHFFYQYNINGAVWGDIAWGHAVSKDLIHWFHLPLAMVPDQWYDANGVWTGSATLLDDGSIVMLYTGSTDNFVQVQNLAYPE DPSDPLLLKWTKFSGYPVLVPPPGIGARDFRDPTTAWKTLAGKWRITIGSKVNRTGISLVYDTTDFKTYEKLDNLLHQVPDTG | |||
| Full Sequence |
|---|
| Protein Sequence Length: 599 Download |
| MASSDALLPV IFQRKPLSDS RSDDLILESR RRQPIKVHRA VILLLVALYV TLIVIHNESA 60 SKNGGSNETT TKSPAPLAGV SEKRNFRKTE PFAWKNTMLS WQRTAFHFQP EKNWMNGPLF 120 YKGLYHFFYQ YNINGAVWGD IAWGHAVSKD LIHWFHLPLA MVPDQWYDAN GVWTGSATLL 180 DDGSIVMLYT GSTDNFVQVQ NLAYPEDPSD PLLLKWTKFS GYPVLVPPPG IGARDFRDPT 240 TAWKTLAGKW RITIGSKVNR TGISLVYDTT DFKTYEKLDN LLHQVPDTGM TYYDSNGTWI 300 PDDPTFDVGK STSLRYDYGK FYASKTFYDQ NKRRRILWGW IGESDSEAAD VKKGWSSVQS 360 IPRTVVLDTK TGKNLVQWPV EEVKSLRLSS KKFDMKVKPE TVVQVDVSST AQLDIEAEFE 420 INKEDLKKIT GDESVEAEED FSCETSGGST VSGALGPFGF SVLTDESLSE QTTVYFYVTK 480 GKDSKLKTFF CTDTLRSTMA NDVVKSVYGS FVPVLKEEKL TMRILVDHSI IEGFGQGGRT 540 CITSRVYPTE ANYGAAKLFL FNNAFNATIT ASFEVWQMNS AFIRPYSAAD LVLLHNSS* 600 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01322 | scrB_fam | 1.0e-44 | 103 | 548 | 495 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| COG1621 | SacC | 1.0e-62 | 103 | 552 | 495 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 3.0e-79 | 113 | 382 | 311 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 4.0e-124 | 107 | 379 | 320 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 7.0e-162 | 107 | 538 | 479 | + Glycosyl hydrolases family 32. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004564 | beta-fructofuranosidase activity |
| GO:0004575 | sucrose alpha-glucosidase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAD43622.1 | 0 | 1 | 591 | 1 | 644 | AC005698_21 T3P18.21 [Arabidopsis thaliana] |
| GenBank | AAF19535.1 | 0 | 1 | 591 | 1 | 644 | AC007190_3 F23N19.3 [Arabidopsis thaliana] |
| GenBank | AAG36942.1 | 0 | 1 | 587 | 1 | 653 | AF274298_1 acid invertase [Brassica oleracea] |
| GenBank | AAK62665.1 | 0 | 1 | 591 | 1 | 642 | At1g62660/F23N19_3 [Arabidopsis thaliana] |
| RefSeq | NP_564798.1 | 0 | 1 | 591 | 1 | 642 | beta-fructosidase (BFRUCT3) / beta-fructofuranosidase / invertase, vacuolar [Arabidopsis thaliana] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3ugh_B | 0 | 91 | 586 | 7 | 544 | A Chain A, Crystal Structure Of Phl P 4, A Grass Pollen Allergen With Glucose Dehydrogenase Activity |
| PDB | 3ugh_A | 0 | 91 | 586 | 7 | 544 | A Chain A, Crystal Structure Of Phl P 4, A Grass Pollen Allergen With Glucose Dehydrogenase Activity |
| PDB | 3ugg_B | 0 | 91 | 586 | 7 | 544 | A Chain A, Crystal Structure Of Phl P 4, A Grass Pollen Allergen With Glucose Dehydrogenase Activity |
| PDB | 3ugg_A | 0 | 91 | 586 | 7 | 544 | A Chain A, Crystal Structure Of Phl P 4, A Grass Pollen Allergen With Glucose Dehydrogenase Activity |
| PDB | 3ugf_B | 0 | 91 | 586 | 7 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| sucrose degradation III | RXN-1461 | EC-3.2.1.26 | β-fructofuranosidase |
