| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra037509 |
| Family | GH28 |
| Protein Properties | Length: 865 Molecular Weight: 95172.4 Isoelectric Point: 7.7125 |
| Chromosome | Chromosome/Scaffold: 06 Start: 21130733 End: 21134502 |
| Description | GRAS family transcription factor |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH28 | 512 | 843 | 0 |
| EACQFPTRSSVLIPKGEYKLRQIEMMGPCKAPIRITLQGTVKADGNVNGNDYWVAFRRINGFKLNGGGIFDGEGNAAWRANNCHKMALTQCKKLPISIRF DFVTDAKIRGITSLDSKNFHINVLGARNMTLEEITIIAPEESPNTDGIHVGRSVGVQIINSNIKTGDDCISIGDGTRDLLVERVTCGPGHGISIGSLGLY VKEEDVTGIRVVNSTLINTDNGVRIKTWPSAACSTTASGIHFENIILKNVTNPILIDQEYCPWNRCNKNKPSTIKLVDISFKHIRGTSGNKDAVKLLCSK GFPCKNVQIGDIDIKYTGADGPATFQCSNVSP | |||
| Full Sequence |
|---|
| Protein Sequence Length: 865 Download |
| MYKHPRQETE AYTLPPFEAN SVGKLRYIPA HNSLKRYCML EPSSSSSLVS PAYAVPSNAT 60 VNSSAHEDTS GSCVTDDFND KIKELETVMM GPDSLVFDYN DSFDSTSCQE TNSWRSTLEA 120 ISRRDLRADL VSCAQALSEN DLMMAHSMME KLRQMVSVSG EPIQRLGAYL LEGLVAKLAS 180 SGSSIYKSLN RFPEPASTEL LSYMHILYEV CPYFKFGYMS ANGAIAEAMK EDNRVHIIDF 240 QIGQGSQWVT LIQAFAARPG GPPRIRITGI DDTTSAYARG GGLSIVGNRL AKLAKQFNVP 300 FEFNSVSVSA SEVKLKDLGV RLGEALAVNF AFVLHHMPDE SVSTENHRDR LLRMVKSLSP 360 KVVTLVEQES NTNTASFFPR FKETMDYYDA MFESIDVTLP RNHKQRINVE QHCLARDVVN 420 IIACEGADRV ERHELLGKWR SRFEMAGFTS YPLSPLVNST IKSLLRNYSD KYRLEERDGA 480 LYLGLSANAK DFTITAPPGS DITSLLLKTF NEACQFPTRS SVLIPKGEYK LRQIEMMGPC 540 KAPIRITLQG TVKADGNVNG NDYWVAFRRI NGFKLNGGGI FDGEGNAAWR ANNCHKMALT 600 QCKKLPISIR FDFVTDAKIR GITSLDSKNF HINVLGARNM TLEEITIIAP EESPNTDGIH 660 VGRSVGVQII NSNIKTGDDC ISIGDGTRDL LVERVTCGPG HGISIGSLGL YVKEEDVTGI 720 RVVNSTLINT DNGVRIKTWP SAACSTTASG IHFENIILKN VTNPILIDQE YCPWNRCNKN 780 KPSTIKLVDI SFKHIRGTSG NKDAVKLLCS KGFPCKNVQI GDIDIKYTGA DGPATFQCSN 840 VSPTLMGTQV PKACSSPVTK LPGQ* 900 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN02793 | PLN02793 | 3.0e-77 | 500 | 864 | 371 | + Probable polygalacturonase | ||
| PLN02155 | PLN02155 | 3.0e-80 | 497 | 854 | 364 | + polygalacturonase | ||
| PLN02188 | PLN02188 | 8.0e-116 | 488 | 854 | 371 | + polygalacturonase/glycoside hydrolase family protein | ||
| pfam00295 | Glyco_hydro_28 | 1.0e-126 | 514 | 854 | 342 | + Glycosyl hydrolases family 28. Glycosyl hydrolase family 28 includes polygalacturonase EC:3.2.1.15 as well as rhamnogalacturonase A(RGase A), EC:3.2.1.-. These enzymes is important in cell wall metabolism. | ||
| pfam03514 | GRAS | 9.0e-173 | 126 | 484 | 363 | + GRAS family transcription factor. Sequence analysis of the products of the GRAS (GAI, RGA, SCR) gene family indicates that they share a variable amino-terminus and a highly conserved carboxyl-terminus that contains five recognisable motifs. Proteins in the GRAS family are transcription factors that seem to be involved in development and other processes. Mutation of the SCARECROW (SCR) gene results in a radial pattern defect, loss of a ground tissue layer, in the root. The PAT1 protein is involved in phytochrome A signal transduction. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004650 | polygalacturonase activity |
| GO:0005975 | carbohydrate metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ABG38467.1 | 0 | 483 | 864 | 16 | 397 | pollen-expressed protein MF6t [Brassica juncea] |
| GenBank | ACP74159.1 | 0 | 483 | 864 | 16 | 397 | putative polygalacturonase [Brassica rapa subsp. campestris] |
| DDBJ | BAC42147.1 | 0 | 89 | 484 | 1 | 398 | putative SCARECROW gene regulator [Arabidopsis thaliana] |
| RefSeq | NP_199626.1 | 0 | 1 | 484 | 1 | 477 | PAT1 (phytochrome a signal transduction 1); signal transducer/ transcription factor [Arabidopsis thaliana] |
| RefSeq | XP_002321179.1 | 0 | 4 | 484 | 19 | 534 | GRAS family transcription factor [Populus trichocarpa] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2iq7_G | 5e-17 | 655 | 841 | 153 | 331 | A Chain A, Crystal Structure Of The Polygalacturonase From Colletotrichum Lupini And Its Implications For The Interaction With Polygalacturonase- Inhibiting Proteins |
| PDB | 2iq7_F | 5e-17 | 655 | 841 | 153 | 331 | A Chain A, Crystal Structure Of The Polygalacturonase From Colletotrichum Lupini And Its Implications For The Interaction With Polygalacturonase- Inhibiting Proteins |
| PDB | 2iq7_E | 5e-17 | 655 | 841 | 153 | 331 | A Chain A, Crystal Structure Of The Polygalacturonase From Colletotrichum Lupini And Its Implications For The Interaction With Polygalacturonase- Inhibiting Proteins |
| PDB | 2iq7_D | 5e-17 | 655 | 841 | 153 | 331 | A Chain A, Crystal Structure Of The Polygalacturonase From Colletotrichum Lupini And Its Implications For The Interaction With Polygalacturonase- Inhibiting Proteins |
| PDB | 2iq7_C | 5e-17 | 655 | 841 | 153 | 331 | A Chain A, Crystal Structure Of The Polygalacturonase From Colletotrichum Lupini And Its Implications For The Interaction With Polygalacturonase- Inhibiting Proteins |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| homogalacturonan degradation | RXN-2103 | EC-3.2.1.15 | polygalacturonase |
| Hydropathy |
|---|
 |
| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| ES268994 | 273 | 485 | 757 | 0 |
| GR457589 | 266 | 530 | 795 | 0 |
| ES270777 | 266 | 483 | 748 | 0 |
| GR450939 | 264 | 598 | 861 | 0 |
| GR455947 | 266 | 563 | 828 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
|---|
 |
