| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra037698 |
| Family | GH19 |
| Protein Properties | Length: 277 Molecular Weight: 29637.3 Isoelectric Point: 9.8886 |
| Chromosome | Chromosome/Scaffold: 04 Start: 18151714 End: 18152892 |
| Description | Chitinase family protein |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH19 | 83 | 276 | 0 |
| IASVVTPAFFNSIMSKVGNGCPAKGFYTRQAFIAAAQSFPAYKGTVAKREIAAMLAQFSHESGSFCYKEEIARGRYCSPSKTYPCQPGKNYYGRGPIQIT WNYNYGAAGKFLGLPLLKDPDMVARSPTVAFKCAMWFWNLNVRPVLSRGFGATTRRINGGECDGGRPAAVQSRVNHYLDFCKKLGVTPGTNLKC | |||
| Full Sequence |
|---|
| Protein Sequence Length: 277 Download |
| MLLKNALIVL LFTLTILAET AFSQNCGKTG CKGNMCCSRW GYCGTTNAYC GTGCQNGPCK 60 SKPKPTPTPS GSGGLNAGPR GTIASVVTPA FFNSIMSKVG NGCPAKGFYT RQAFIAAAQS 120 FPAYKGTVAK REIAAMLAQF SHESGSFCYK EEIARGRYCS PSKTYPCQPG KNYYGRGPIQ 180 ITWNYNYGAA GKFLGLPLLK DPDMVARSPT VAFKCAMWFW NLNVRPVLSR GFGATTRRIN 240 GGECDGGRPA AVQSRVNHYL DFCKKLGVTP GTNLKC* 300 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| smart00270 | ChtBD1 | 6.0e-8 | 25 | 57 | 37 | + Chitin binding domain. | ||
| COG3179 | COG3179 | 2.0e-10 | 114 | 242 | 166 | + Predicted chitinase [General function prediction only] | ||
| cd00442 | lysozyme_like | 8.0e-11 | 134 | 244 | 111 | + lysozyme_like domain. This contains several members including Soluble Lytic Transglycosylases (SLT), Goose Egg-White Lysozymes (GEWL), Hen Egg-White Lysozymes (HEWL), chitinases, bacteriophage lambda lysozymes, endolysins, autolysins, and chitosanases. All the members are involved in the hydrolysis of beta-1,4- linked polysaccharides. | ||
| pfam00182 | Glyco_hydro_19 | 4.0e-75 | 86 | 276 | 232 | + Chitinase class I. | ||
| cd00325 | chitinase_glyco_hydro_19 | 3.0e-92 | 87 | 276 | 230 | + Glycoside hydrolase family 19 chitinase domain. Chitinases are enzymes that catalyze the hydrolysis of the beta-1,4-N-acetyl-D-glucosamine linkages in chitin polymers. Family 19 chitinases are found primarily in plants (classes I, III, and IV), but some are found in bacteria. Class I and II chitinases are similar in their catalytic domains. Class I chitinases have an N-terminal cysteine-rich, chitin-binding domain which is separated from the catalytic domain by a proline and glycine-rich hinge region. Class II chitinases lack both the chitin-binding domain and the hinge region. Class IV chitinases are similar to class I chitinases but they are smaller in size due to certain deletions. Despite any significant sequence homology with lysozymes, structural analysis reveals that family 19 chitinases, together with family 46 chitosanases, are similar to several lysozymes including those from T4-phage and from goose. The structures reveal that the different enzyme groups arose from a common ancestor glycohydrolase antecedent to the procaryotic/eucaryotic divergence. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004568 | chitinase activity |
| GO:0006032 | chitin catabolic process |
| GO:0008061 | chitin binding |
| GO:0016998 | cell wall macromolecule catabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| RefSeq | NP_176061.1 | 0 | 2 | 276 | 7 | 280 | glycoside hydrolase family 19 protein [Arabidopsis thaliana] |
| RefSeq | NP_181887.1 | 0 | 16 | 276 | 17 | 264 | chitinase, putative [Arabidopsis thaliana] |
| RefSeq | NP_181888.1 | 0 | 1 | 276 | 1 | 273 | glycoside hydrolase family 19 protein [Arabidopsis thaliana] |
| RefSeq | NP_181889.1 | 0 | 2 | 276 | 7 | 281 | glycoside hydrolase family 19 protein [Arabidopsis thaliana] |
| RefSeq | NP_181890.1 | 0 | 2 | 276 | 7 | 283 | chitinase, putative [Arabidopsis thaliana] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3hbh_A | 0 | 82 | 276 | 1 | 204 | A Chain A, Bacillus Subtilis Pectate Lyase |
| PDB | 3hbe_X | 0 | 82 | 276 | 1 | 204 | A Chain A, Bacillus Subtilis Pectate Lyase |
| PDB | 3hbd_A | 0 | 82 | 276 | 1 | 204 | A Chain A, Class Iv Chitinase Structure From Picea Abies At 1.8a |
| PDB | 4dyg_B | 0 | 82 | 276 | 2 | 237 | A Chain A, Class Iv Chitinase Structure From Picea Abies At 1.8a |
| PDB | 4dyg_A | 0 | 82 | 276 | 2 | 237 | A Chain A, Class Iv Chitinase Structure From Picea Abies At 1.8a |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| chitin degradation II | 3.2.1.14-RXN | EC-3.2.1.14 | chitinase |
| chitin degradation II | RXN-12623 | EC-3.2.1.14 | chitinase |
| chitin degradation II | RXN-12624 | EC-3.2.1.14 | chitinase |
| chitin degradation III (carnivorous plants) | 3.2.1.14-RXN | EC-3.2.1.14 | chitinase |
