| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra038363 |
| Family | CE10 |
| Protein Properties | Length: 338 Molecular Weight: 37274.9 Isoelectric Point: 6.4279 |
| Chromosome | Chromosome/Scaffold: 02 Start: 10969867 End: 10970880 |
| Description | alpha/beta-Hydrolases superfamily protein |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| CE10 | 63 | 316 | 0 |
| VHIDELTNVWARLYVPTVTKSLSVSSSKLPLLVYFHDGGFCVGSASWSCYHEFLARLSASSRCIIMSVNYRLAPENPLPAAYEDGVNAILWLKKTRNDNL WSKLCDFGKIFLTGDSAGGNIANHVAARLQAVDALIQPLKIEGTILIQPFFGGEARTESERRVENNMKSSVLTLAASDAWWRLALPRGANREHPYCKPAK SKTRTLVCVAEMDVLMDREMEMCDEIDNEEMIKRVVYKGVGHAFQILGKSQLAQ | |||
| Full Sequence |
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| Protein Sequence Length: 338 Download |
| MGATTVTHVT TINPTNTNIH GPAIDEVDGL IRVYKDGHVE RFQLVPCVGP SLPLDLAVAC 60 SDVHIDELTN VWARLYVPTV TKSLSVSSSK LPLLVYFHDG GFCVGSASWS CYHEFLARLS 120 ASSRCIIMSV NYRLAPENPL PAAYEDGVNA ILWLKKTRND NLWSKLCDFG KIFLTGDSAG 180 GNIANHVAAR LQAVDALIQP LKIEGTILIQ PFFGGEARTE SERRVENNMK SSVLTLAASD 240 AWWRLALPRG ANREHPYCKP AKSKTRTLVC VAEMDVLMDR EMEMCDEIDN EEMIKRVVYK 300 GVGHAFQILG KSQLAQTMTL EMLCHIDGFI HQCDPSI* 360 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| COG2272 | PnbA | 0.0001 | 96 | 196 | 114 | + Carboxylesterase type B [Lipid metabolism] | ||
| cd00312 | Esterase_lipase | 2.0e-5 | 96 | 187 | 106 | + Esterases and lipases (includes fungal lipases, cholinesterases, etc.) These enzymes act on carboxylic esters (EC: 3.1.1.-). The catalytic apparatus involves three residues (catalytic triad): a serine, a glutamate or aspartate and a histidine.These catalytic residues are responsible for the nucleophilic attack on the carbonyl carbon atom of the ester bond. In contrast with other alpha/beta hydrolase fold family members, p-nitrobenzyl esterase and acetylcholine esterase have a Glu instead of Asp at the active site carboxylate. | ||
| PRK10162 | PRK10162 | 3.0e-11 | 96 | 306 | 218 | + acetyl esterase; Provisional | ||
| COG0657 | Aes | 1.0e-26 | 96 | 319 | 231 | + Esterase/lipase [Lipid metabolism] | ||
| pfam07859 | Abhydrolase_3 | 2.0e-58 | 96 | 308 | 221 | + alpha/beta hydrolase fold. This catalytic domain is found in a very wide range of enzymes. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0008152 | metabolic process |
| GO:0016787 | hydrolase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| RefSeq | NP_564936.1 | 0 | 1 | 335 | 1 | 334 | hydrolase [Arabidopsis thaliana] |
| RefSeq | XP_002278939.1 | 0 | 16 | 330 | 22 | 345 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002311450.1 | 0 | 20 | 330 | 23 | 346 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002315949.1 | 0 | 20 | 330 | 21 | 343 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002529140.1 | 0 | 20 | 330 | 22 | 350 | catalytic, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2o7v_A | 0 | 58 | 291 | 53 | 292 | A Chain A, Catalytic Function And Substrate Recognition Of The Pectate Lyase From Thermotoga Maritima |
| PDB | 2o7r_A | 0 | 58 | 291 | 53 | 292 | A Chain A, Plant Carboxylesterase Aecxe1 From Actinidia Eriantha With Acyl Adduct |
| PDB | 2zsi_A | 1e-37 | 53 | 286 | 66 | 307 | A Chain A, Plant Carboxylesterase Aecxe1 From Actinidia Eriantha With Acyl Adduct |
| PDB | 2zsh_A | 1e-37 | 53 | 286 | 66 | 307 | B Chain B, Structural Basis Of Gibberellin(Ga3)-Induced Della Recognition By The Gibberellin Receptor |
| PDB | 3ed1_F | 4e-35 | 87 | 298 | 108 | 318 | B Chain B, Structural Basis Of Gibberellin(Ga3)-Induced Della Recognition By The Gibberellin Receptor |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| formononetin biosynthesis | RXN-3284 | EC-4.2.1.105 | 2-hydroxyisoflavanone dehydratase |
| formononetin biosynthesis | RXN-3625 | - | 2,7-dihydroxy-4'-methoxyisoflavanone dehydratase |
| isoflavonoid biosynthesis I | RXN-3284 | EC-4.2.1.105 | 2-hydroxyisoflavanone dehydratase |
| isoflavonoid biosynthesis II | RXN-3303 | EC-4.2.1.105 | 2-hydroxyisoflavanone dehydratase |
| isoflavonoid biosynthesis II | RXN-5502 | - | 2,7,5-trihydroxy-4'-methoxyisoflavanone dehydratase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| DK480522 | 286 | 46 | 331 | 0 |
| FY439288 | 259 | 21 | 278 | 0 |
| GR439665 | 224 | 1 | 224 | 0 |
| EH426322 | 219 | 118 | 335 | 0 |
| EX131404 | 250 | 1 | 250 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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