| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra040077 |
| Family | GH28 |
| Protein Properties | Length: 366 Molecular Weight: 39511.3 Isoelectric Point: 6.3611 |
| Chromosome | Chromosome/Scaffold: 08 Start: 13088255 End: 13091495 |
| Description | Pectin lyase-like superfamily protein |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH28 | 52 | 251 | 0 |
| KALCAGKSKSLVIHANEVYTLLPQVFQGPCVASNPHIQIDGKIEAPKLVKDWGSNKPENWLSFDKVSGLTITGSGLLDPHGESWWSTVKFDSRPQALQFN RCNNLIYNGLTQRNSPKNHISISNCIQATLSNLHLIAPANSPNTDGIDISLSQQINIFSSAIQTGSLGEGGATETVENVHVQHCTFTGADNAARIKTWPG | |||
| Full Sequence |
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| Protein Sequence Length: 366 Download |
| MGFQLLISVL LVSSFYAHYG DSDSEFISVR DFLSDSGNTN ADQSQAIQDA WKALCAGKSK 60 SLVIHANEVY TLLPQVFQGP CVASNPHIQI DGKIEAPKLV KDWGSNKPEN WLSFDKVSGL 120 TITGSGLLDP HGESWWSTVK FDSRPQALQF NRCNNLIYNG LTQRNSPKNH ISISNCIQAT 180 LSNLHLIAPA NSPNTDGIDI SLSQQINIFS SAIQTGSLGE GGATETVENV HVQHCTFTGA 240 DNAARIKTWP GSAVKVSDVT FRYFRGTCAQ PIAIKLDCDK IGCGNIVLEH INITSSSPQT 300 SPSAICRFAD VTSSFVNIDM KCVNHADLQG PSPSPELPSQ YDQPHAQLPQ ASAPHAQARP 360 FLSFL* 420 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN02188 | PLN02188 | 4.0e-36 | 17 | 307 | 369 | + polygalacturonase/glycoside hydrolase family protein | ||
| PLN02793 | PLN02793 | 4.0e-42 | 37 | 297 | 310 | + Probable polygalacturonase | ||
| pfam00295 | Glyco_hydro_28 | 5.0e-45 | 55 | 314 | 333 | + Glycosyl hydrolases family 28. Glycosyl hydrolase family 28 includes polygalacturonase EC:3.2.1.15 as well as rhamnogalacturonase A(RGase A), EC:3.2.1.-. These enzymes is important in cell wall metabolism. | ||
| PLN03003 | PLN03003 | 1.0e-45 | 37 | 297 | 333 | + Probable polygalacturonase At3g15720 | ||
| PLN03010 | PLN03010 | 4.0e-65 | 37 | 324 | 359 | + polygalacturonase | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004650 | polygalacturonase activity |
| GO:0005975 | carbohydrate metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| RefSeq | NP_194963.2 | 0 | 3 | 262 | 11 | 305 | glycoside hydrolase family 28 protein / polygalacturonase (pectinase) family protein [Arabidopsis thaliana] |
| RefSeq | NP_194964.2 | 0 | 99 | 350 | 1 | 332 | glycoside hydrolase family 28 protein / polygalacturonase (pectinase) family protein [Arabidopsis thaliana] |
| RefSeq | NP_680757.1 | 0 | 45 | 362 | 4 | 395 | glycoside hydrolase family 28 protein / polygalacturonase (pectinase) family protein [Arabidopsis thaliana] |
| RefSeq | XP_002324855.1 | 0 | 3 | 315 | 6 | 386 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002515526.1 | 0 | 26 | 323 | 27 | 392 | Polygalacturonase-2 precursor, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1bhe_A | 0.0002 | 122 | 274 | 113 | 310 | A Chain A, Polygalacturonase From Erwinia Carotovora Ssp. Carotovora |
| PDB | 1nhc_F | 0.0005 | 120 | 312 | 72 | 284 | A Chain A, Structural Insights Into The Processivity Of Endopolygalacturonase I From Aspergillus Niger |
| PDB | 1nhc_E | 0.0005 | 120 | 312 | 72 | 284 | A Chain A, Structural Insights Into The Processivity Of Endopolygalacturonase I From Aspergillus Niger |
| PDB | 1nhc_D | 0.0005 | 120 | 312 | 72 | 284 | A Chain A, Structural Insights Into The Processivity Of Endopolygalacturonase I From Aspergillus Niger |
| PDB | 1nhc_C | 0.0005 | 120 | 312 | 72 | 284 | A Chain A, Structural Insights Into The Processivity Of Endopolygalacturonase I From Aspergillus Niger |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| homogalacturonan degradation | RXN-2103 | EC-3.2.1.15 | polygalacturonase |
| Signal Peptide | ||||
|---|---|---|---|---|
| Cleavage Site | ||||
| 22 | ||||
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| CV538127 | 226 | 61 | 252 | 1.4013e-45 |
| CV536748 | 255 | 92 | 278 | 3.00018e-42 |
| DY961682 | 253 | 37 | 249 | 1.99993e-41 |
| DY964976 | 191 | 37 | 216 | 2e-39 |
| EY950069 | 195 | 25 | 216 | 5e-38 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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| Phylogeny |
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