| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra040667 |
| Family | CE10 |
| Protein Properties | Length: 320 Molecular Weight: 35382.7 Isoelectric Point: 5.5169 |
| Chromosome | Chromosome/Scaffold: 05 Start: 13140968 End: 13141927 |
| Description | carboxyesterase 5 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| CE10 | 37 | 317 | 0 |
| TNDVVSKDVQYSREHNLSVRLFLPHKATAGTNSKKLPLLIYIHGGAWIIESPFSPTYHNYVADVVTSANCLAVSIQYRRAPEHPVPASYEDSWSAVEWIF SHSDGSGPEDWINEHADFGRVFIGGDSAGANMTHHMAMRAGKEKLDPKIKGIAVVHPAFWGTDPVDEHDVQDREMRSGIAQLWEKVVSPNSVNGTDDPLF NVVGSDSDLSGLGCEMVLVAVAGKDVFVRQGLAYAAKLKKSGWKGDVEVVEEEEEGHVFHLQNPHSENASKFFERFVKFIT | |||
| Full Sequence |
|---|
| Protein Sequence Length: 320 Download |
| MESDLVSEHP PFIRIYKDGR VERLTGTETI PTSLDKTNDV VSKDVQYSRE HNLSVRLFLP 60 HKATAGTNSK KLPLLIYIHG GAWIIESPFS PTYHNYVADV VTSANCLAVS IQYRRAPEHP 120 VPASYEDSWS AVEWIFSHSD GSGPEDWINE HADFGRVFIG GDSAGANMTH HMAMRAGKEK 180 LDPKIKGIAV VHPAFWGTDP VDEHDVQDRE MRSGIAQLWE KVVSPNSVNG TDDPLFNVVG 240 SDSDLSGLGC EMVLVAVAGK DVFVRQGLAY AAKLKKSGWK GDVEVVEEEE EGHVFHLQNP 300 HSENASKFFE RFVKFITTP* 360 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd00312 | Esterase_lipase | 2.0e-5 | 53 | 181 | 146 | + Esterases and lipases (includes fungal lipases, cholinesterases, etc.) These enzymes act on carboxylic esters (EC: 3.1.1.-). The catalytic apparatus involves three residues (catalytic triad): a serine, a glutamate or aspartate and a histidine.These catalytic residues are responsible for the nucleophilic attack on the carbonyl carbon atom of the ester bond. In contrast with other alpha/beta hydrolase fold family members, p-nitrobenzyl esterase and acetylcholine esterase have a Glu instead of Asp at the active site carboxylate. | ||
| COG2272 | PnbA | 2.0e-6 | 53 | 166 | 125 | + Carboxylesterase type B [Lipid metabolism] | ||
| PRK10162 | PRK10162 | 2.0e-6 | 53 | 168 | 117 | + acetyl esterase; Provisional | ||
| COG0657 | Aes | 9.0e-29 | 55 | 318 | 276 | + Esterase/lipase [Lipid metabolism] | ||
| pfam07859 | Abhydrolase_3 | 7.0e-55 | 75 | 297 | 229 | + alpha/beta hydrolase fold. This catalytic domain is found in a very wide range of enzymes. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0008152 | metabolic process |
| GO:0016787 | hydrolase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAM61628.1 | 0 | 10 | 317 | 66 | 373 | putative esterase [Arabidopsis thaliana] |
| GenBank | AAT68324.1 | 0 | 1 | 318 | 1 | 315 | hypothetical protein At1g49640 [Arabidopsis thaliana] |
| RefSeq | NP_175387.1 | 0 | 1 | 318 | 1 | 315 | hydrolase [Arabidopsis thaliana] |
| RefSeq | NP_175389.1 | 0 | 1 | 316 | 1 | 317 | AtCXE5 (Arabidopsis thaliana carboxyesterase 5); carboxylesterase |
| RefSeq | NP_564550.1 | 0 | 10 | 317 | 66 | 373 | cell death associated protein-related [Arabidopsis thaliana] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2o7v_A | 2e-36 | 25 | 317 | 41 | 328 | A Chain A, The Crystal Structure Of Rice (Oryza Sativa L.) Os4bglu12 |
| PDB | 2o7r_A | 2e-36 | 25 | 317 | 41 | 328 | A Chain A, Plant Carboxylesterase Aecxe1 From Actinidia Eriantha With Acyl Adduct |
| PDB | 2zsi_A | 1e-25 | 30 | 314 | 60 | 349 | A Chain A, Plant Carboxylesterase Aecxe1 From Actinidia Eriantha With Acyl Adduct |
| PDB | 2zsh_A | 1e-25 | 30 | 314 | 60 | 349 | B Chain B, Structural Basis Of Gibberellin(Ga3)-Induced Della Recognition By The Gibberellin Receptor |
| PDB | 3ed1_F | 6e-24 | 69 | 318 | 109 | 349 | B Chain B, Structural Basis Of Gibberellin(Ga3)-Induced Della Recognition By The Gibberellin Receptor |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| formononetin biosynthesis | RXN-3284 | EC-4.2.1.105 | 2-hydroxyisoflavanone dehydratase |
| formononetin biosynthesis | RXN-3625 | - | 2,7-dihydroxy-4'-methoxyisoflavanone dehydratase |
| isoflavonoid biosynthesis I | RXN-3284 | EC-4.2.1.105 | 2-hydroxyisoflavanone dehydratase |
| isoflavonoid biosynthesis II | RXN-3303 | EC-4.2.1.105 | 2-hydroxyisoflavanone dehydratase |
| isoflavonoid biosynthesis II | RXN-5502 | - | 2,7,5-trihydroxy-4'-methoxyisoflavanone dehydratase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| DK458597 | 269 | 1 | 269 | 0 |
| GR719789 | 249 | 1 | 249 | 0 |
| EX114228 | 233 | 1 | 233 | 0 |
| GR722718 | 228 | 1 | 228 | 0 |
| CD813428 | 234 | 2 | 235 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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