| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra040677 |
| Family | AA7 |
| Protein Properties | Length: 506 Molecular Weight: 55604.4 Isoelectric Point: 5.824 |
| Chromosome | Chromosome/Scaffold: 000232 Start: 36707 End: 41121 |
| Description | cytokinin oxidase 2 |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| AA7 | 55 | 244 | 3.8e-30 |
| YGNVTAVTPGGVLCPSSSAEISRLLRYAANGDTIFQVAARGQGHSLNGQASVSGGVVINMTCLADITVSEDKEYADVAGGTLWVDVLRETAEQGVSPVSW TDYLHISVGGTLSNAGIGGQMFRNGPQISNVLELDVITGKGETLACSPQLNPELFYGVLGGLGQFGIISRARIVLNHAPKRAKWFKMLYS | |||
| Full Sequence |
|---|
| Protein Sequence Length: 506 Download |
| MANLRLMITL ITVLVYLLPS FAKSSESTKV ITLPKSLNLT LLTDPSTIST ASHDYGNVTA 60 VTPGGVLCPS SSAEISRLLR YAANGDTIFQ VAARGQGHSL NGQASVSGGV VINMTCLADI 120 TVSEDKEYAD VAGGTLWVDV LRETAEQGVS PVSWTDYLHI SVGGTLSNAG IGGQMFRNGP 180 QISNVLELDV ITGKGETLAC SPQLNPELFY GVLGGLGQFG IISRARIVLN HAPKRAKWFK 240 MLYSDFTAFT KDQESLISMN NDTGVDYVEG QQLMSNGIVD TSFFPPSDQS KVADLVKNHS 300 IIYVLEVAKY YDDPTVPTTG QVIDKLTKTL SYLPGFISMH DVPYFDFLNR VQAEEDKLRS 360 LALWEVPHPW LNLYVPKTQI LDFHNGVVKD ILLKQNSTSG VALFYPTNRN KWDNRMSAMI 420 PDEDVFYVVG LLQSASSQNV QEVESVNEAI ISFCKDSGIK IKQYLMHFTR KEDWVGHFGS 480 KWGAFSKRKD LFDPKKLLSP GQDIF* 540 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01679 | bact_FAD_ox | 1.0e-8 | 56 | 238 | 190 | + FAD-linked oxidoreductase. This model represents a family of bacterial oxidoreductases with covalently linked FAD, closely related to two different eukaryotic oxidases, L-gulonolactone oxidase (EC 1.1.3.8) from rat and D-arabinono-1,4-lactone oxidase (EC 1.1.3.37) from Saccharomyces cerevisiae. | ||
| pfam01565 | FAD_binding_4 | 3.0e-26 | 63 | 201 | 142 | + FAD binding domain. This family consists of various enzymes that use FAD as a co-factor, most of the enzymes are similar to oxygen oxidoreductase. One of the enzymes Vanillyl-alcohol oxidase (VAO) has a solved structure, the alignment includes the FAD binding site, called the PP-loop, between residues 99-110. The FAD molecule is covalently bound in the known structure, however the residue that links to the FAD is not in the alignment. VAO catalyzes the oxidation of a wide variety of substrates, ranging form aromatic amines to 4-alkylphenols. Other members of this family include D-lactate dehydrogenase, this enzyme catalyzes the conversion of D-lactate to pyruvate using FAD as a co-factor; mitomycin radical oxidase, this enzyme oxidises the reduced form of mitomycins and is involved in mitomycin resistance. This family includes MurB an UDP-N-acetylenolpyruvoylglucosamine reductase enzyme EC:1.1.1.158. This enzyme is involved in the biosynthesis of peptidoglycan. | ||
| COG0277 | GlcD | 1.0e-30 | 39 | 502 | 480 | + FAD/FMN-containing dehydrogenases [Energy production and conversion] | ||
| pfam09265 | Cytokin-bind | 9.0e-147 | 233 | 505 | 281 | + Cytokinin dehydrogenase 1, FAD and cytokinin binding. Members of this family adopt an alpha+beta sandwich structure with an antiparallel beta-sheet, in a ferredoxin-like fold. They are predominantly found in plant cytokinin dehydrogenase 1, where they are capable of binding both FAD and cytokinin substrates. The substrate displays a 'plug-into-socket' binding mode that seals the catalytic site and precisely positions the carbon atom undergoing oxidation in close contact with the reactive locus of the flavin. | ||
| PLN02441 | PLN02441 | 0 | 1 | 505 | 520 | + cytokinin dehydrogenase | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0008762 | UDP-N-acetylmuramate dehydrogenase activity |
| GO:0009690 | cytokinin metabolic process |
| GO:0016491 | oxidoreductase activity |
| GO:0019139 | cytokinin dehydrogenase activity |
| GO:0050660 | flavin adenine dinucleotide binding |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAG30905.1 | 0 | 1 | 505 | 1 | 501 | AF303978_1 cytokinin oxidase [Arabidopsis thaliana] |
| GenBank | AAG30907.1 | 0 | 7 | 505 | 9 | 523 | AF303980_1 cytokinin oxidase [Arabidopsis thaliana] |
| RefSeq | NP_194703.1 | 0 | 7 | 505 | 9 | 523 | CKX4 (CYTOKININ OXIDASE 4); amine oxidase/ cytokinin dehydrogenase [Arabidopsis thaliana] |
| RefSeq | NP_565455.1 | 0 | 1 | 505 | 1 | 501 | CKX2 (CYTOKININ OXIDASE 2); amine oxidase/ cytokinin dehydrogenase [Arabidopsis thaliana] |
| RefSeq | NP_849470.1 | 0 | 7 | 411 | 9 | 428 | CKX4 (CYTOKININ OXIDASE 4); amine oxidase/ cytokinin dehydrogenase [Arabidopsis thaliana] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3s1e_A | 0 | 36 | 505 | 24 | 515 | A Chain A, Pro427gln Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenine |
| PDB | 3s1c_A | 0 | 36 | 505 | 24 | 515 | A Chain A, Pro427gln Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenine |
| PDB | 3dq0_A | 0 | 36 | 505 | 24 | 515 | A Chain A, Pro427gln Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenine |
| PDB | 3c0p_A | 0 | 36 | 505 | 24 | 515 | A Chain A, Pro427gln Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenine |
| PDB | 3bw7_A | 0 | 36 | 505 | 24 | 515 | A Chain A, Pro427gln Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenine |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| cytokinins degradation | RXN-4621 | EC-1.5.99 | isopentenyladenine:FAD oxidoreductase |
| cytokinins degradation | RXN-4661 | EC-1.5.99 | trans-zeatin:FAD oxidoreductase |
