| Basic Information | |
|---|---|
| Species | Brachypodium distachyon |
| Cazyme ID | Bradi1g52210.1 |
| Family | GH32 |
| Protein Properties | Length: 613 Molecular Weight: 67885.1 Isoelectric Point: 5.5829 |
| Chromosome | Chromosome/Scaffold: 1 Start: 50586062 End: 50590075 |
| Description | Glycosyl hydrolases family 32 protein |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
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| Family | Start | End | Evalue |
| GH32 | 90 | 399 | 0 |
| HFQPEKNWMNDPNGPVYYRGWYHLFYQYNPEGAVWGNIAWGHAVSRDLVHWRHLPLALVPDRWYDVNGVWTGSATVLPDGSLVMLYTGSTNASVQVQCLA LPADPDDSLLRNWTKHDANPVLLPPPRIGHRDFRDPTTAWFDESDRTWRTVIGSKDNRGHAGIALVYKTKDFVRYDLIPGLLHRVDGTGMWECIDFYPVG DKEELHVMKASMDDDRHDYYALGKYDAKANTWTPIDPEADVGLGLRYDWGKFYASKTFYDPAKKRRVLWGWVGETDSERADVAKGWASLQSLPRTVALDD KTRTNLLQWP | |||
| Full Sequence |
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| Protein Sequence Length: 613 Download |
| MAEDYQQQPG RRAIVPGSPP LPYAYASLPP EDDGDLRRRT AGTGGERWRA CAAVLAASAV 60 VLMVGAGAGA DADGFPWSNA MLQWQFTGFH FQPEKNWMND PNGPVYYRGW YHLFYQYNPE 120 GAVWGNIAWG HAVSRDLVHW RHLPLALVPD RWYDVNGVWT GSATVLPDGS LVMLYTGSTN 180 ASVQVQCLAL PADPDDSLLR NWTKHDANPV LLPPPRIGHR DFRDPTTAWF DESDRTWRTV 240 IGSKDNRGHA GIALVYKTKD FVRYDLIPGL LHRVDGTGMW ECIDFYPVGD KEELHVMKAS 300 MDDDRHDYYA LGKYDAKANT WTPIDPEADV GLGLRYDWGK FYASKTFYDP AKKRRVLWGW 360 VGETDSERAD VAKGWASLQS LPRTVALDDK TRTNLLQWPV EEVETLRMNS TSFSGITIDH 420 GSVFPLSLRR ATQLDIEATF RLSPSAVAAL TEADVTYNCS TSGGAAHRGA LGPFGLLLLA 480 TPNPGEQTAA YFYVTRGTDG ELRTHFCHDE SRSSKANDIV KRVVGSTVPV LDGEGLSMRV 540 LVDHSIVESF VMGGRSTVTS RVYPTEAIYA KAGVFIFNNA TAGAVTVEKL VVHEMDRSYN 600 QAFMDDDTTT DL* |
| Functional Domains Download unfiltered results here | ||||||||
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| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01322 | scrB_fam | 5.0e-59 | 86 | 564 | 499 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| COG1621 | SacC | 4.0e-74 | 89 | 580 | 516 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 1.0e-93 | 96 | 402 | 318 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 1.0e-151 | 90 | 399 | 320 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 1.0e-177 | 90 | 554 | 476 | + Glycosyl hydrolases family 32. | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAL87233.1 | 0 | 18 | 607 | 5 | 647 | AF481763_1 fructosyltransferase [Lolium perenne] |
| GenBank | AAS88729.1 | 0 | 63 | 612 | 104 | 657 | vacuolar invertase1 [Triticum monococcum] |
| GenBank | ABB77251.1 | 0 | 64 | 604 | 99 | 657 | vacuolar invertase BObetaFRUCT3 [Bambusa oldhamii] |
| GenBank | ACS92722.1 | 0 | 1 | 612 | 1 | 656 | sucrose:sucrose 1-fructosyltransferase [Brachypodium distachyon] |
| EMBL | CAD58683.1 | 0 | 78 | 607 | 1 | 532 | putative soluble acid invertase [Lolium temulentum] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3ugh_B | 0 | 75 | 603 | 8 | 544 | A Chain A, Arabidopsis Thaliana Peroxidase A2 |
| PDB | 3ugh_A | 0 | 75 | 603 | 8 | 544 | A Chain A, Arabidopsis Thaliana Peroxidase A2 |
| PDB | 3ugg_B | 0 | 75 | 603 | 8 | 544 | A Chain A, Arabidopsis Thaliana Peroxidase A2 |
| PDB | 3ugg_A | 0 | 75 | 603 | 8 | 544 | A Chain A, Arabidopsis Thaliana Peroxidase A2 |
| PDB | 3ugf_B | 0 | 75 | 603 | 8 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| fructan biosynthesis | RXN-1781 | EC-2.4.1.99 | sucrose:sucrose fructosyltransferase |
| fructan biosynthesis | RXN-1801 | EC-2.4.1.100 | 2,1-fructan:2,1-fructan 1-fructosyltransferase |
| fructan biosynthesis | RXN-1803 | EC-2.4.1.10 | levansucrase |
| fructan biosynthesis | RXN-1804 | EC-2.4.1.10 | levansucrase |
| sucrose degradation III | RXN-1461 | EC-3.2.1.26 | β-fructofuranosidase |
