Basic Information | |
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Species | Brachypodium distachyon |
Cazyme ID | Bradi2g19640.1 |
Family | GT20 |
Protein Properties | Length: 969 Molecular Weight: 107695 Isoelectric Point: 6.6504 |
Chromosome | Chromosome/Scaffold: 2 Start: 17284780 End: 17292225 |
Description | trehalose-6-phosphate synthase |
View CDS |
External Links |
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NCBI Taxonomy |
Plaza |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GT20 | 117 | 585 | 0 |
MKQRLLVVANRLPVSANRRGEDQWSLEISAGGLVSALLGVKDVDAKWIGWAGVNVPDEVGQRALTKALAEKRCIPVFLDEEIVHQYYNGYCNNILWPLFH YLGLPQEDRLATTRNFESQFDAYKRANQMFADVVYQHYKEGDVIWCHDYHLMFLPKCLKDHDINMKVGWFLHTPFPSSEIYRTLPSRSELLRSVLCADLV GFHTYDYARHFVSACTRILGLEGTPEGVEDQGKLTRVAAFPIGIDSDRFKRALEIPGVKRHISELTQRFAGRKVMLGVDRLDMIKGIPQKILAFEKFLEE NPDWNDKVVLLQIAVPTRTDVPEYQKLTSQVHEIVGRINGRFGTLTAVPIHHLDRSLDFHALCALYAVTDVALVTSLRDGMNLVSYEYVACQGSKKGVLI LSEFAGAAQSLGAGAILVNPWNITEVADSINHALTMTSDEREKRHRHNYAHVTTHTAQDWAETFVCELN |
Full Sequence |
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Protein Sequence Length: 969 Download |
MPTPSSNNSP AASSPFSNDG GGGAPASPNR VERMLRERGH RHLFSTGGAS SPDAMDTDAA 60 EPAAASFADF GAARSPDAAE PANMDDAGGG GAPGGHAARP PLAGPRSGFR RLGLRGMKQR 120 LLVVANRLPV SANRRGEDQW SLEISAGGLV SALLGVKDVD AKWIGWAGVN VPDEVGQRAL 180 TKALAEKRCI PVFLDEEIVH QYYNGYCNNI LWPLFHYLGL PQEDRLATTR NFESQFDAYK 240 RANQMFADVV YQHYKEGDVI WCHDYHLMFL PKCLKDHDIN MKVGWFLHTP FPSSEIYRTL 300 PSRSELLRSV LCADLVGFHT YDYARHFVSA CTRILGLEGT PEGVEDQGKL TRVAAFPIGI 360 DSDRFKRALE IPGVKRHISE LTQRFAGRKV MLGVDRLDMI KGIPQKILAF EKFLEENPDW 420 NDKVVLLQIA VPTRTDVPEY QKLTSQVHEI VGRINGRFGT LTAVPIHHLD RSLDFHALCA 480 LYAVTDVALV TSLRDGMNLV SYEYVACQGS KKGVLILSEF AGAAQSLGAG AILVNPWNIT 540 EVADSINHAL TMTSDEREKR HRHNYAHVTT HTAQDWAETF VCELNDTVAE AQMRTRQVPP 600 VLPSRTAIQQ YLHSRNRLLI LGFNSTLTEP VESSGRRGGD QIKEMELKLH PDLKGPLRAL 660 CEDEHTTVIV LSGSDRSVLD ENFGEFNLWL AAEHGMFLRP TDGEWMTTMP EHLNMDWVDS 720 VKHVFEYFTE RTPRSHFEHR ETSFVWNYKY ADVEFGRLQA RDMLQHLWTG PISNAAVDVV 780 QGSRSVEVRS VGVTKGAAID RILGEIVHSK SMVTPIDYVL CIGHFLGKDE DIYVFFDPEY 840 PSESKVKPDG ASVSVDRRQN GRPSNGRSNS RNSQSRTQKS QVAPERSSSS SHGTTGNNHH 900 DWREGSSVLD LNGDNYFSCA VGRKRSNARY LLNSSEDVVS FLKEMAESTT PRAGFQSSAA 960 DYMFLDRQ* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
PLN03064 | PLN03064 | 0 | 20 | 951 | 939 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
PLN03063 | PLN03063 | 0 | 115 | 950 | 836 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
TIGR02400 | trehalose_OtsA | 0 | 120 | 586 | 469 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming]. This enzyme catalyzes the key, penultimate step in biosynthesis of trehalose, a compatible solute made as an osmoprotectant in some species in all three domains of life. The gene symbol OtsA stands for osmotically regulated trehalose synthesis A. Trehalose helps protect against both osmotic and thermal stresses, and is made from two glucose subunits. This model excludes glucosylglycerol-phosphate synthase, an enzyme of an analogous osmoprotectant system in many cyanobacterial strains. This model does not identify archaeal examples, as they are more divergent than glucosylglycerol-phosphate synthase. Sequences that score in the gray zone between the trusted and noise cutoffs include a number of yeast multidomain proteins in which the N-terminal domain may be functionally equivalent to this family. The gray zone also includes the OtsA of Cornyebacterium glutamicum (and related species), shown to be responsible for synthesis of only trace amounts of trehalose while the majority is synthesized by the TreYZ pathway; the significance of OtsA in this species is unclear (see Wolf, et al., PMID:12890033) [Cellular processes, Adaptations to atypical conditions]. | ||
pfam00982 | Glyco_transf_20 | 0 | 119 | 586 | 474 | + Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex. | ||
PRK14501 | PRK14501 | 0 | 120 | 823 | 709 | + putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional |
Gene Ontology | |
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GO Term | Description |
GO:0003824 | catalytic activity |
GO:0005992 | trehalose biosynthetic process |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | ABF70084.1 | 0 | 4 | 948 | 3 | 959 | trehalose-6-phosphate synthase, putative [Musa balbisiana] |
GenBank | ACI16353.1 | 0 | 117 | 968 | 1 | 859 | trehalose-6-P synthase [Triticum aestivum] |
GenBank | EAY98715.1 | 0 | 117 | 968 | 1 | 855 | hypothetical protein OsI_20647 [Oryza sativa Indica Group] |
RefSeq | NP_001123593.2 | 0 | 13 | 963 | 4 | 935 | trehalose-6-phosphate synthase [Zea mays] |
RefSeq | XP_002440086.1 | 0 | 13 | 968 | 27 | 977 | hypothetical protein SORBIDRAFT_09g025790 [Sorghum bicolor] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 2wtx_D | 0 | 120 | 596 | 3 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
PDB | 2wtx_C | 0 | 120 | 596 | 3 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
PDB | 2wtx_B | 0 | 120 | 596 | 3 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
PDB | 2wtx_A | 0 | 120 | 596 | 3 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
PDB | 1uqu_B | 0 | 120 | 596 | 3 | 466 | A Chain A, Trehalose-6-Phosphate From E. Coli Bound With Udp-Glucose. |
Metabolic Pathways | |||
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Pathway Name | Reaction | EC | Protein Name |
trehalose biosynthesis I | TREHALOSE6PSYN-RXN | EC-2.4.1.15 | α,α-trehalose-phosphate synthase (UDP-forming) |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
CB651133 | 281 | 146 | 426 | 0 |
DW069910 | 288 | 206 | 493 | 0 |
EH189441 | 291 | 488 | 778 | 0 |
CB629539 | 271 | 298 | 568 | 0 |
CV711307 | 293 | 118 | 410 | 0 |
Sequence Alignments (This image is cropped. Click for full image.) |
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