Basic Information | |
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Species | Brachypodium distachyon |
Cazyme ID | Bradi2g55120.1 |
Family | GT35 |
Protein Properties | Length: 834 Molecular Weight: 93856.9 Isoelectric Point: 7.4695 |
Chromosome | Chromosome/Scaffold: 2 Start: 53826480 End: 53833267 |
Description | alpha-glucan phosphorylase 2 |
View CDS |
External Links |
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NCBI Taxonomy |
Plaza |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GT35 | 105 | 827 | 0 |
ALKKFGYELEAIAGQERDAALGNGGLGRLASCFLDSMATLNLPAWGYGLRYRYGLFKQIIAKEGQEEFAEDWLDKFSPWEIVRHDVVYPIRFFGHVEISP DGTRKWAGGEVMSALAYDVPIPGYKTKNAISLRLWDAKATAQDFNLFQFNDGQYESAAQLHSRAQQICAVLYPGDATEEGKLLRLKQQYFLCSASLQDII FRFKERKPDRVSGKWSEFPSKVAVQMNDTHPTLAIPELMRLLMDEEGLGWDEAWDVTNKTVAYTNHTVLPEALEKWSQTVMRKLLPRHMEIIEEIDKRFR EMVISTRKDMEGKIESMRVLDNSNPEKPVVRMANLCVVSSHTVNGVAELHSNILKEELFADYVSIWPKKFQNKTNGITPRRWLRFCNPELSEIVTKWLKT DQWTSNLDLLIGLRKFADDEKLHAEWAAAKLASKKRLAKHVLDVTGVTIDPNSLFDIQIKRIHEYKRQLLNILGAVYRYKKLKEMSAEEKQKVTPRTVMV GGKAFATYTNAKRIVKLVNDVGAVVNNDPDVNKYLKVVFIPNYNVSVAEVLIPGSELSQHISTAGMEASGTSNMKFSLNGCLIIGTLDGANVEIREEVGQ DNFFLFGAKADQIAGLRKDRENGLFKPDPRFEEAKQLVRSGAFGSYDYTPLLDSLEGNSGFGRGDYFLVGYDFPSYIEAQARVDEAYKDKKRWIKMSILN TAGSGKFSSDRTIDQYAKEIWGI |
Full Sequence |
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Protein Sequence Length: 834 Download |
MSAADKVKPA ATPGSADPAA IAGNISYHAQ YSPHFSPFAF GPEQAFFSTA ESVRDHLLQR 60 WNDTYVHFHK TDPKQTYYLS MEYLQGRALS NAVGSLGITG AYAEALKKFG YELEAIAGQE 120 RDAALGNGGL GRLASCFLDS MATLNLPAWG YGLRYRYGLF KQIIAKEGQE EFAEDWLDKF 180 SPWEIVRHDV VYPIRFFGHV EISPDGTRKW AGGEVMSALA YDVPIPGYKT KNAISLRLWD 240 AKATAQDFNL FQFNDGQYES AAQLHSRAQQ ICAVLYPGDA TEEGKLLRLK QQYFLCSASL 300 QDIIFRFKER KPDRVSGKWS EFPSKVAVQM NDTHPTLAIP ELMRLLMDEE GLGWDEAWDV 360 TNKTVAYTNH TVLPEALEKW SQTVMRKLLP RHMEIIEEID KRFREMVIST RKDMEGKIES 420 MRVLDNSNPE KPVVRMANLC VVSSHTVNGV AELHSNILKE ELFADYVSIW PKKFQNKTNG 480 ITPRRWLRFC NPELSEIVTK WLKTDQWTSN LDLLIGLRKF ADDEKLHAEW AAAKLASKKR 540 LAKHVLDVTG VTIDPNSLFD IQIKRIHEYK RQLLNILGAV YRYKKLKEMS AEEKQKVTPR 600 TVMVGGKAFA TYTNAKRIVK LVNDVGAVVN NDPDVNKYLK VVFIPNYNVS VAEVLIPGSE 660 LSQHISTAGM EASGTSNMKF SLNGCLIIGT LDGANVEIRE EVGQDNFFLF GAKADQIAGL 720 RKDRENGLFK PDPRFEEAKQ LVRSGAFGSY DYTPLLDSLE GNSGFGRGDY FLVGYDFPSY 780 IEAQARVDEA YKDKKRWIKM SILNTAGSGK FSSDRTIDQY AKEIWGITAS PVP* 840 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
cd04300 | GT1_Glycogen_Phosphorylase | 0 | 24 | 827 | 812 | + This is a family of oligosaccharide phosphorylases. It includes yeast and mammalian glycogen phosphorylases, plant starch/glucan phosphorylase, as well as the maltodextrin phosphorylases of bacteria. The members of this family catalyze the breakdown of oligosaccharides into glucose-1-phosphate units. They are important allosteric enzymes in carbohydrate metabolism. The allosteric control mechanisms of yeast and mammalian members of this family are different from that of bacterial members. The members of this family belong to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. | ||
TIGR02093 | P_ylase | 0 | 25 | 827 | 811 | + glycogen/starch/alpha-glucan phosphorylases. This family consists of phosphorylases. Members use phosphate to break alpha 1,4 linkages between pairs of glucose residues at the end of long glucose polymers, releasing alpha-D-glucose 1-phosphate. The nomenclature convention is to preface the name according to the natural substrate, as in glycogen phosphorylase, starch phosphorylase, maltodextrin phosphorylase, etc. Name differences among these substrates reflect differences in patterns of branching with alpha 1,6 linkages. Members include allosterically regulated and unregulated forms. A related family, TIGR02094, contains examples known to act well on particularly small alpha 1,4 glucans, as may be found after import from exogenous sources [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
pfam00343 | Phosphorylase | 0 | 105 | 827 | 728 | + Carbohydrate phosphorylase. The members of this family catalyze the formation of glucose 1-phosphate from one of the following polyglucoses; glycogen, starch, glucan or maltodextrin. | ||
COG0058 | GlgP | 0 | 17 | 827 | 815 | + Glucan phosphorylase [Carbohydrate transport and metabolism] | ||
PRK14985 | PRK14985 | 0 | 78 | 825 | 757 | + maltodextrin phosphorylase; Provisional |
Gene Ontology | |
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GO Term | Description |
GO:0004645 | phosphorylase activity |
GO:0005975 | carbohydrate metabolic process |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | AAG45939.1 | 0 | 25 | 832 | 1 | 808 | alpha 1,4-glucan phosphorylase H isozyme [Oryza sativa] |
GenBank | EEC71819.1 | 0 | 3 | 832 | 11 | 840 | hypothetical protein OsI_04460 [Oryza sativa Indica Group] |
RefSeq | NP_001044823.1 | 0 | 3 | 832 | 11 | 840 | Os01g0851700 [Oryza sativa (japonica cultivar-group)] |
Swiss-Prot | Q9LKJ3 | 0 | 1 | 833 | 1 | 832 | PHSH_WHEAT RecName: Full=Alpha-glucan phosphorylase, H isozyme; AltName: Full=Starch phosphorylase H |
RefSeq | XP_002458773.1 | 0 | 3 | 832 | 8 | 837 | hypothetical protein SORBIDRAFT_03g040060 [Sorghum bicolor] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 1z8d_A | 0 | 14 | 830 | 21 | 831 | A Chain A, Crystal Structure Of Human Muscle Glycogen Phosphorylase A With Amp And Glucose |
PDB | 4el5_A | 0 | 14 | 830 | 9 | 819 | A Chain A, Crystal Structure Of Human Muscle Glycogen Phosphorylase A With Amp And Glucose |
PDB | 4el0_A | 0 | 14 | 830 | 9 | 819 | A Chain A, Crystal Structure Of Human Muscle Glycogen Phosphorylase A With Amp And Glucose |
PDB | 4eky_A | 0 | 14 | 830 | 9 | 819 | A Chain A, Crystal Structure Of Human Muscle Glycogen Phosphorylase A With Amp And Glucose |
PDB | 4eke_A | 0 | 14 | 830 | 9 | 819 | A Chain A, Crystal Structure Of Human Muscle Glycogen Phosphorylase A With Amp And Glucose |
Metabolic Pathways | |||
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Pathway Name | Reaction | EC | Protein Name |
starch degradation I | RXN-1826 | EC-2.4.1.1 | phosphorylase |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
HO782200 | 413 | 161 | 573 | 0 |
HO418036 | 539 | 231 | 749 | 0 |
GT044838 | 338 | 467 | 804 | 0 |
GT043461 | 330 | 452 | 781 | 0 |
GO871118 | 329 | 135 | 463 | 0 |
Sequence Alignments (This image is cropped. Click for full image.) |
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