Basic Information | |
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Species | Brachypodium distachyon |
Cazyme ID | Bradi3g00910.1 |
Family | GH32 |
Protein Properties | Length: 672 Molecular Weight: 72824.5 Isoelectric Point: 5.0468 |
Chromosome | Chromosome/Scaffold: 3 Start: 495382 End: 498849 |
Description | Glycosyl hydrolases family 32 protein |
View CDS |
External Links |
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NCBI Taxonomy |
Plaza |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH32 | 131 | 459 | 0 |
HFQPEKNWMNDPNGPVYYKGWYHLFYQYNPTGAIWGNKIAWGHAVSRDLLRWRHLPIAMSPDQWYDINGVWTGSATVLPNGTLAMLYTGSTNASVQVQCL AFPSDPEDPLLIEWTKDERNPVMYPPTEIGERDFRDPTTAWRDPEDDTWRIVIGSKDAHHAGIAMTYKTIDFVNYDLVPGLLHRVPATGMWECIDLYPVS GKHGIDMTAAMAASSNEGGGEETVYVMKASMDDDRHDYYALGKYDAKANKWTPLDEEADVGIGLRYDWGKFYASKTFYDPAKKRRVLWGWVGETDSERAD VAKGWASLQSLPRTVVLDTKTGSNLLQWP |
Full Sequence |
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Protein Sequence Length: 672 Download |
MEARDGGSPA LPCSSYAALP DDAEAAAQPG RGRRRASGGA VCAAVLLTTA AVLLSLAALA 60 GVRLAGHLPA NGGVLEEQPA AAVLGSRGPE AGVSEKTSGA AVDVRLGAGA GEENAFPWSN 120 AMLQWQRTGF HFQPEKNWMN DPNGPVYYKG WYHLFYQYNP TGAIWGNKIA WGHAVSRDLL 180 RWRHLPIAMS PDQWYDINGV WTGSATVLPN GTLAMLYTGS TNASVQVQCL AFPSDPEDPL 240 LIEWTKDERN PVMYPPTEIG ERDFRDPTTA WRDPEDDTWR IVIGSKDAHH AGIAMTYKTI 300 DFVNYDLVPG LLHRVPATGM WECIDLYPVS GKHGIDMTAA MAASSNEGGG EETVYVMKAS 360 MDDDRHDYYA LGKYDAKANK WTPLDEEADV GIGLRYDWGK FYASKTFYDP AKKRRVLWGW 420 VGETDSERAD VAKGWASLQS LPRTVVLDTK TGSNLLQWPV DEVETLRTNS TDFGGVTVDH 480 GSVFPLRLHR ATQLDILAEF RLDPLDIAAA QEADVGYNCS TSGGAAGQGA LGPFGLLVLA 540 DARHHGDGTE QTAVYFYVAR GLDGRLHTHF CQDETRSSRA NEIVKRVVGN VVPVLDGEAL 600 SVRVLVDHSI VESFAQGGRS TATSRVYPTE AIYANAGVYL FNNATGARVT ATSLVVHEMD 660 SSYNQAYMAS L* |
Functional Domains Download unfiltered results here | ||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description |
TIGR01322 | scrB_fam | 1.0e-60 | 127 | 628 | 516 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. |
COG1621 | SacC | 6.0e-77 | 127 | 644 | 540 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] |
cd08996 | GH32_B_Fructosidase | 1.0e-94 | 137 | 462 | 333 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. |
pfam00251 | Glyco_hydro_32N | 1.0e-151 | 131 | 459 | 336 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. |
smart00640 | Glyco_32 | 0 | 131 | 618 | 497 | + Glycosyl hydrolases family 32. |
Gene Ontology | |
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GO Term | Description |
GO:0004564 | beta-fructofuranosidase activity |
GO:0004575 | sucrose alpha-glucosidase activity |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | AAL92880.1 | 0 | 1 | 671 | 3 | 670 | fructosyltransferase [Lolium perenne] |
GenBank | ABB77251.1 | 0 | 82 | 669 | 75 | 658 | vacuolar invertase BObetaFRUCT3 [Bambusa oldhamii] |
EMBL | CAD58681.1 | 0 | 1 | 671 | 3 | 677 | putative soluble acid invertase [Lolium temulentum] |
EMBL | CAF22241.1 | 0 | 15 | 662 | 11 | 657 | soluble acid invertase [Hordeum vulgare] |
EMBL | CAG25609.1 | 0 | 1 | 671 | 1 | 673 | acid beta-fructofuranosidase precursor [Triticum aestivum] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3ugh_B | 0 | 116 | 667 | 8 | 544 | A Chain A, Golgi Alpha-mannosidase Ii In Complex With Kifunensine |
PDB | 3ugh_A | 0 | 116 | 667 | 8 | 544 | A Chain A, Golgi Alpha-mannosidase Ii In Complex With Kifunensine |
PDB | 3ugg_B | 0 | 116 | 667 | 8 | 544 | A Chain A, Golgi Alpha-mannosidase Ii In Complex With Kifunensine |
PDB | 3ugg_A | 0 | 116 | 667 | 8 | 544 | A Chain A, Golgi Alpha-mannosidase Ii In Complex With Kifunensine |
PDB | 3ugf_B | 0 | 116 | 667 | 8 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
Metabolic Pathways | |||
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Pathway Name | Reaction | EC | Protein Name |
fructan biosynthesis | RXN-1781 | EC-2.4.1.99 | sucrose:sucrose fructosyltransferase |
fructan biosynthesis | RXN-1801 | EC-2.4.1.100 | 2,1-fructan:2,1-fructan 1-fructosyltransferase |
fructan biosynthesis | RXN-1803 | EC-2.4.1.10 | levansucrase |
fructan biosynthesis | RXN-1804 | EC-2.4.1.10 | levansucrase |
sucrose degradation III | RXN-1461 | EC-3.2.1.26 | β-fructofuranosidase |