| Basic Information | |
|---|---|
| Species | Brachypodium distachyon |
| Cazyme ID | Bradi3g10310.1 |
| Family | GH16 |
| Protein Properties | Length: 290 Molecular Weight: 32677.7 Isoelectric Point: 4.709 |
| Chromosome | Chromosome/Scaffold: 3 Start: 8495196 End: 8496661 |
| Description | xyloglucan endotransglucosylase/hydrolase 16 |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH16 | 36 | 206 | 3.6e-25 |
| NAYFSPDGHSLTMSLPRENSGCALQTKKQFIYGSVSTLIKLIPGDSAGTVTTYYTSSAMDSNKKHDEIDFEFLGNETGQPYTIHTNIFVDGVGNREMQFV PWFNATDGYHNYTISWTPCMVVWYVDSVPIRVFRNFSKEGVPFPVTRPMYAYSTLWEADDWATQGGRVKTN | |||
| Full Sequence |
|---|
| Protein Sequence Length: 290 Download |
| MESSRALLLM AVTVAAIGLA GANFRDDCDI PWAPENAYFS PDGHSLTMSL PRENSGCALQ 60 TKKQFIYGSV STLIKLIPGD SAGTVTTYYT SSAMDSNKKH DEIDFEFLGN ETGQPYTIHT 120 NIFVDGVGNR EMQFVPWFNA TDGYHNYTIS WTPCMVVWYV DSVPIRVFRN FSKEGVPFPV 180 TRPMYAYSTL WEADDWATQG GRVKTNWANA PFVASYQDMV VDVCSCASGD YYCADNCKAK 240 YYGYDQQYYC GGLQPDQAKA MLELQQKYRI YDYCVKYRAA NKECDLQQY* |
| Functional Domains Download unfiltered results here | ||||||
|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description |
| cd02175 | GH16_lichenase | 3.0e-24 | 32 | 197 | 174 | + lichenase, member of glycosyl hydrolase family 16. Lichenase, also known as 1,3-1,4-beta-glucanase, is a member of glycosyl hydrolase family 16, that specifically cleaves 1,4-beta-D-glucosidic bonds in mixed-linked beta glucans that also contain 1,3-beta-D-glucosidic linkages. Natural substrates of beta-glucanase are beta-glucans from grain endosperm cell walls or lichenan from the Islandic moss, Cetraria islandica. This protein is found not only in bacteria but also in anaerobic fungi. This domain includes two seven-stranded antiparallel beta-sheets that are adjacent to one another forming a compact, jellyroll beta-sandwich structure. |
| cd00413 | Glyco_hydrolase_16 | 5.0e-26 | 32 | 206 | 186 | + glycosyl hydrolase family 16. The O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycosyl hydrolase family 16. Family 16 includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues. |
| pfam00722 | Glyco_hydro_16 | 6.0e-68 | 24 | 208 | 186 | + Glycosyl hydrolases family 16. |
| PLN03161 | PLN03161 | 2.0e-89 | 1 | 274 | 279 | + Probable xyloglucan endotransglucosylase/hydrolase protein; Provisional |
| cd02176 | GH16_XET | 2.0e-116 | 20 | 284 | 268 | + Xyloglucan endotransglycosylase, member of glycosyl hydrolase family 16. Xyloglucan endotransglycosylases (XETs) cleave and religate xyloglucan polymers in plant cell walls via a transglycosylation mechanism. Xyloglucan is a soluble hemicellulose with a backbone of beta-1,4-linked glucose units, partially substituted with alpha-1,6-linked xylopyranose branches. It binds noncovalently to cellulose, cross-linking the adjacent cellulose microfibrils, giving it a key structural role as a matrix polymer. Therefore, XET plays an important role in all plant processes that require cell wall remodeling. |
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0005975 | carbohydrate metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | EAY85374.1 | 0 | 25 | 288 | 26 | 291 | hypothetical protein OsI_06752 [Oryza sativa Indica Group] |
| GenBank | EAZ00792.1 | 0 | 23 | 289 | 25 | 309 | hypothetical protein OsI_22822 [Oryza sativa Indica Group] |
| RefSeq | NP_001046555.1 | 0 | 25 | 288 | 26 | 291 | Os02g0280300 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | XP_002438432.1 | 0 | 3 | 289 | 4 | 294 | hypothetical protein SORBIDRAFT_10g019390 [Sorghum bicolor] |
| RefSeq | XP_002451953.1 | 0 | 18 | 289 | 18 | 290 | hypothetical protein SORBIDRAFT_04g010980 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1un1_B | 0 | 32 | 284 | 25 | 272 | A Chain A, Crystal Structure Of The Nasturtium Seedling Mutant Xyloglucanase Isoform Nxg1-Delta-Yniig |
| PDB | 1un1_A | 0 | 32 | 284 | 25 | 272 | A Chain A, Crystal Structure Of The Nasturtium Seedling Mutant Xyloglucanase Isoform Nxg1-Delta-Yniig |
| PDB | 1umz_B | 0 | 32 | 284 | 25 | 272 | A Chain A, Xyloglucan Endotransglycosylase In Complex With The Xyloglucan Nonasaccharide Xllg. |
| PDB | 1umz_A | 0 | 32 | 284 | 25 | 272 | A Chain A, Xyloglucan Endotransglycosylase In Complex With The Xyloglucan Nonasaccharide Xllg. |
| PDB | 2uwb_B | 0 | 32 | 274 | 28 | 254 | A Chain A, Crystal Structure Of The Nasturtium Seedling Mutant Xyloglucanase Isoform Nxg1-Delta-Yniig |
