| Basic Information | |
|---|---|
| Species | Brachypodium distachyon |
| Cazyme ID | Bradi3g18607.1 |
| Family | GH16 |
| Protein Properties | Length: 292 Molecular Weight: 33411.4 Isoelectric Point: 4.6358 |
| Chromosome | Chromosome/Scaffold: 3 Start: 17137901 End: 17139270 |
| Description | Xyloglucan endotransglucosylase/hydrolase family protein |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH16 | 64 | 215 | 2.4e-24 |
| GGSGFHTKEAYLYARFDIDIMLVPKNSAGTVTTLYLMPEDVPWDYHDEVDLEFLGNVTGEPYTLHTNVFANGVGSREEQFRLWFDPTADFHTYSVDWNPK RMTILVDGVPIRNFKNRAGMPFMTWQKMRLHGSLWNADDWATQGGRVKTDWS | |||
| Full Sequence |
|---|
| Protein Sequence Length: 292 Download |
| MASFREHCSL LLWLVLVAMG QKMAMASIYD DVEVNWGQDH SFFYTDGGGD ELQTLALCLD 60 QSRGGSGFHT KEAYLYARFD IDIMLVPKNS AGTVTTLYLM PEDVPWDYHD EVDLEFLGNV 120 TGEPYTLHTN VFANGVGSRE EQFRLWFDPT ADFHTYSVDW NPKRMTILVD GVPIRNFKNR 180 AGMPFMTWQK MRLHGSLWNA DDWATQGGRV KTDWSEAPFF ASYRNLRVTW CQPSPGVAWC 240 GDEPPESTRF DEGLDADALR QARDKHMIYD YCKDEKRYKQ GSELPKECTS D* 300 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd00413 | Glyco_hydrolase_16 | 2.0e-18 | 69 | 213 | 150 | + glycosyl hydrolase family 16. The O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycosyl hydrolase family 16. Family 16 includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues. | ||
| cd02183 | GH16_fungal_CRH1_transglycosylase | 2.0e-20 | 89 | 229 | 159 | + glycosylphosphatidylinositol-glucanosyltransferase. Group of fungal GH16 members related to Saccharomyces cerevisiae Crh1p. Chr1p and Crh2p are transglycosylases that are required for the linkage of chitin to beta(1-3)glucose branches of beta(1-6)glucan, an important step in the assembly of new cell wall. Both have been shown to be glycosylphosphatidylinositol (GPI)-anchored. A third homologous protein, Crr1p, functions in the formation of the spore wall. They belongs to the family 16 of glycosyl hydrolases that includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues. | ||
| pfam00722 | Glyco_hydro_16 | 3.0e-64 | 29 | 215 | 189 | + Glycosyl hydrolases family 16. | ||
| PLN03161 | PLN03161 | 3.0e-89 | 1 | 288 | 300 | + Probable xyloglucan endotransglucosylase/hydrolase protein; Provisional | ||
| cd02176 | GH16_XET | 7.0e-119 | 26 | 288 | 271 | + Xyloglucan endotransglycosylase, member of glycosyl hydrolase family 16. Xyloglucan endotransglycosylases (XETs) cleave and religate xyloglucan polymers in plant cell walls via a transglycosylation mechanism. Xyloglucan is a soluble hemicellulose with a backbone of beta-1,4-linked glucose units, partially substituted with alpha-1,6-linked xylopyranose branches. It binds noncovalently to cellulose, cross-linking the adjacent cellulose microfibrils, giving it a key structural role as a matrix polymer. Therefore, XET plays an important role in all plant processes that require cell wall remodeling. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0005618 | cell wall |
| GO:0005975 | carbohydrate metabolic process |
| GO:0006073 | cellular glucan metabolic process |
| GO:0016762 | xyloglucan:xyloglucosyl transferase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | EAZ06117.1 | 0 | 30 | 289 | 40 | 298 | hypothetical protein OsI_28354 [Oryza sativa Indica Group] |
| GenBank | EAZ06118.1 | 0 | 30 | 289 | 36 | 294 | hypothetical protein OsI_28355 [Oryza sativa Indica Group] |
| GenBank | EAZ41980.1 | 0 | 28 | 289 | 1 | 261 | hypothetical protein OsJ_26529 [Oryza sativa Japonica Group] |
| RefSeq | NP_001061321.1 | 0 | 30 | 289 | 41 | 299 | Os08g0237800 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | XP_002444081.1 | 0 | 30 | 289 | 23 | 280 | hypothetical protein SORBIDRAFT_07g007010 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1un1_B | 0 | 36 | 291 | 25 | 275 | A Chain A, Pro427gln Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenine |
| PDB | 1un1_A | 0 | 36 | 291 | 25 | 275 | A Chain A, Pro427gln Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenine |
| PDB | 1umz_B | 0 | 36 | 291 | 25 | 275 | A Chain A, Xyloglucan Endotransglycosylase In Complex With The Xyloglucan Nonasaccharide Xllg. |
| PDB | 1umz_A | 0 | 36 | 291 | 25 | 275 | A Chain A, Xyloglucan Endotransglycosylase In Complex With The Xyloglucan Nonasaccharide Xllg. |
| PDB | 2uwb_B | 8.00001e-42 | 54 | 277 | 39 | 259 | A Chain A, Crystal Structure Of The Nasturtium Seedling Mutant Xyloglucanase Isoform Nxg1-Delta-Yniig |
