| Basic Information | |
|---|---|
| Species | Brachypodium distachyon |
| Cazyme ID | Bradi3g26810.1 |
| Family | GH18 |
| Protein Properties | Length: 291 Molecular Weight: 31296.4 Isoelectric Point: 8.8674 |
| Chromosome | Chromosome/Scaffold: 3 Start: 27566304 End: 27567562 |
| Description | |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH18 | 63 | 206 | 9.7e-22 |
| LHHVHGSPDPNGRPAKRLNPNVRVAVSLGGATVSGKPVFFNATSVDSWVSNAVESLTAIVRRYGLDGIDIDYEQFQADPATFAACAGRLVAGLKRAGVIS SASIAPYGNAEVQRYYRALWADQHVHGADIDYVNFQFYAYGAST | |||
| Full Sequence |
|---|
| Protein Sequence Length: 291 Download |
| MGSSKLTTAV ILLLLPALLA PMATAASNSN LFRDYIGAMY NGVRFTDVPV DPRVRVDYIR 60 HRLHHVHGSP DPNGRPAKRL NPNVRVAVSL GGATVSGKPV FFNATSVDSW VSNAVESLTA 120 IVRRYGLDGI DIDYEQFQAD PATFAACAGR LVAGLKRAGV ISSASIAPYG NAEVQRYYRA 180 LWADQHVHGA DIDYVNFQFY AYGASTTAAR YVSLFDEQMA NYPGANILAS FTTAATTTSV 240 PVDAALSACR TLQSQGKLYG VFLWAADHSK KQGFKYETEV QALLANATGY * 300 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| smart00636 | Glyco_18 | 3.0e-7 | 77 | 200 | 136 | + Glyco_18 domain. | ||
| cd06548 | GH18_chitinase | 7.0e-8 | 78 | 135 | 58 | + The GH18 (glycosyl hydrolases, family 18) type II chitinases hydrolyze chitin, an abundant polymer of N-acetylglucosamine and have been identified in bacteria, fungi, insects, plants, viruses, and protozoan parasites. The structure of this domain is an eight-stranded alpha/beta barrel with a pronounced active-site cleft at the C-terminal end of the beta-barrel. | ||
| cd00598 | GH18_chitinase-like | 9.0e-12 | 78 | 200 | 127 | + The GH18 (glycosyl hydrolase, family 18) type II chitinases hydrolyze chitin, an abundant polymer of beta-1,4-linked N-acetylglucosamine (GlcNAc) which is a major component of the cell wall of fungi and the exoskeleton of arthropods. Chitinases have been identified in viruses, bacteria, fungi, protozoan parasites, insects, and plants. The structure of the GH18 domain is an eight-stranded beta/alpha barrel with a pronounced active-site cleft at the C-terminal end of the beta-barrel. The GH18 family includes chitotriosidase, chitobiase, hevamine, zymocin-alpha, narbonin, SI-CLP (stabilin-1 interacting chitinase-like protein), IDGF (imaginal disc growth factor), CFLE (cortical fragment-lytic enzyme) spore hydrolase, the type III and type V plant chitinases, the endo-beta-N-acetylglucosaminidases, and the chitolectins. The GH85 (glycosyl hydrolase, family 85) ENGases (endo-beta-N-acetylglucosaminidases) are closely related to the GH18 chitinases and are included in this alignment model. | ||
| pfam00704 | Glyco_hydro_18 | 9.0e-14 | 81 | 202 | 129 | + Glycosyl hydrolases family 18. | ||
| cd06544 | GH18_narbonin | 3.0e-77 | 31 | 269 | 257 | + Narbonin is a plant 2S protein from the globulin fraction of narbon bean (Vicia narbonensis L.) cotyledons with unknown function. Narbonin has a glycosyl hydrolase family 18 (GH18) domain without the conserved catalytic residues and with no known enzymatic activity. Narbonin amounts to up to 3% of the total seed globulins of mature seeds and was thought to be a storage protein but was found to degrade too slowly during germination. This family also includes the VfNOD32 nodulin from Vicia faba. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0005975 | carbohydrate metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAO15366.1 | 0 | 28 | 288 | 2 | 279 | AF330230_1 chitinase [Oryza sativa Japonica Group] |
| GenBank | ACF88258.1 | 0 | 21 | 287 | 23 | 305 | unknown [Zea mays] |
| RefSeq | NP_001064605.1 | 0 | 28 | 288 | 27 | 304 | Os10g0416100 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | NP_001152001.1 | 0 | 21 | 287 | 23 | 305 | chitinase 2 [Zea mays] |
| RefSeq | XP_002467243.1 | 0 | 1 | 287 | 1 | 306 | hypothetical protein SORBIDRAFT_01g021920 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3sim_B | 0 | 31 | 284 | 2 | 273 | A Chain A, Crystallographic Structure Analysis Of Family 18 Chitinase From Crocus Vernus |
| PDB | 3sim_A | 0 | 31 | 284 | 2 | 273 | A Chain A, Crystallographic Structure Analysis Of Family 18 Chitinase From Crocus Vernus |
| PDB | 1nar_A | 1e-20 | 78 | 285 | 72 | 288 | A Chain A, Crystal Structure Of Narbonin Refined At 1.8 Angstroms Resolution |
| PDB | 3qok_A | 0.0003 | 63 | 203 | 82 | 230 | A Chain A, Crystal Structure Of Putative Chitinase Ii From Klebsiella Pneumoniae |
| PDB | 1wno_B | 0.001 | 78 | 140 | 84 | 144 | A Chain A, Crystal Structure Of A Native Chitinase From Aspergillus Fumigatus Yj- 407 |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| chitin degradation II | 3.2.1.14-RXN | EC-3.2.1.14 | chitinase |
| chitin degradation II | RXN-12623 | EC-3.2.1.14 | chitinase |
| chitin degradation II | RXN-12624 | EC-3.2.1.14 | chitinase |
| chitin degradation III (carnivorous plants) | 3.2.1.14-RXN | EC-3.2.1.14 | chitinase |
