Basic Information | |
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Species | Brachypodium distachyon |
Cazyme ID | Bradi3g44760.1 |
Family | GH13 |
Protein Properties | Length: 836 Molecular Weight: 94212.6 Isoelectric Point: 5.947 |
Chromosome | Chromosome/Scaffold: 3 Start: 46716583 End: 46727241 |
Description | starch branching enzyme 2.2 |
View CDS |
External Links |
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NCBI Taxonomy |
Plaza |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH13 | 350 | 670 | 8.8e-33 |
LPRIKKLGYNAVQIMAIQEHSYYGSFGYHVTNFFAPSSRFGTPEDLKSLIDRAHELGLVVLMDVVHSHASSNTLDGLNGFDGTDTHYFHGGARGHHWMWD SRVFNYGNWEVIRYLLSNARWWLEEYKFDGFRFDGVTSMMYTHHGLQVTFSGDYNEYFGFNTDVDAVVYLMLVNDLIHGLYPEAITIGEDVSGMPTFALP VQVGGVGFDYRLHMAVADKWIELFKRSDESWQMGDIVHTLTNRRWLEKCVTYAESHDQALVGDKTIAFWLMDKDMYDFMALDRPSTPNIDRGIALHKMIR LITMGLGGEGYLNFMGNEFGH |
Full Sequence |
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Protein Sequence Length: 836 Download |
MMASPAFAVV SGAGLGGAPA PPRSGAADRR RRGLQLPLLF GRDKGSCFPH AIRVASSGRR 60 VVMRVGGVSG EVTIPDGKSD GMLSSDGSAQ FQSDDLEVPS IDDELSIEGG NEGNIESSET 120 DQGTEESNVE GMNKMAKESA AGEKLRVIPP PGNGEQIYEI DPTLKAYKYH LEYRYSLYRR 180 VRSDIDQHEG GMDAFSRGYE NFGFNRSAEG ITYREWAPGA LSAALVGDFN NWDPNADRMS 240 KNDFGIWEIF LPNNADGSTP IPHGSRVKVR MDTPSGIKDS IPAWIKYSVQ APGDIPYNGI 300 YYDPPEDEKY VFKHPQPKRP KSLRIYETHV GMSSPEPKIN TYANFRDEVL PRIKKLGYNA 360 VQIMAIQEHS YYGSFGYHVT NFFAPSSRFG TPEDLKSLID RAHELGLVVL MDVVHSHASS 420 NTLDGLNGFD GTDTHYFHGG ARGHHWMWDS RVFNYGNWEV IRYLLSNARW WLEEYKFDGF 480 RFDGVTSMMY THHGLQVTFS GDYNEYFGFN TDVDAVVYLM LVNDLIHGLY PEAITIGEDV 540 SGMPTFALPV QVGGVGFDYR LHMAVADKWI ELFKRSDESW QMGDIVHTLT NRRWLEKCVT 600 YAESHDQALV GDKTIAFWLM DKDMYDFMAL DRPSTPNIDR GIALHKMIRL ITMGLGGEGY 660 LNFMGNEFGH PEWIDFPRGP QVLPSGKFIP GNNNSYDKCR RRFDLGDAEF LRYHGMQLFD 720 EAMQHLEEKY GYMTSGHQYI SRKHEEDKVI VFEKGELVFV FNFHCSNSYF DYRVGCLKPG 780 KYKVVLDSDA GVFGGFGRIH HTADHFTSDC QHDNRPHSFS VYTPSRTCVV YAPMN* 840 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
PLN02960 | PLN02960 | 2.0e-9 | 165 | 251 | 93 | + alpha-amylase | ||
PLN03244 | PLN03244 | 5.0e-135 | 261 | 831 | 577 | + alpha-amylase; Provisional | ||
PLN02447 | PLN02447 | 0 | 138 | 833 | 700 | + 1,4-alpha-glucan-branching enzyme | ||
cd11321 | AmyAc_bac_euk_BE | 0 | 306 | 720 | 416 | + Alpha amylase catalytic domain found in bacterial and eukaryotic branching enzymes. Branching enzymes (BEs) catalyze the formation of alpha-1,6 branch points in either glycogen or starch by cleavage of the alpha-1,4 glucosidic linkage yielding a non-reducing end oligosaccharide chain, and subsequent attachment to the alpha-1,6 position. By increasing the number of non-reducing ends, glycogen is more reactive to synthesis and digestion as well as being more soluble. This group includes bacterial and eukaryotic proteins. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
PLN02960 | PLN02960 | 0 | 261 | 831 | 576 | + alpha-amylase |
Gene Ontology | |
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GO Term | Description |
GO:0003824 | catalytic activity |
GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
GO:0005975 | carbohydrate metabolic process |
GO:0043169 | cation binding |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | AAC69754.1 | 0 | 19 | 835 | 16 | 829 | starch branching enzyme IIb [Hordeum vulgare subsp. vulgare] |
GenBank | AAW80631.1 | 0 | 19 | 835 | 16 | 836 | starch branching enzyme IIb [Triticum aestivum] |
GenBank | ACE88265.1 | 0 | 2 | 833 | 1 | 823 | rice starch branching enzyme [Oryza sativa Japonica Group] |
GenBank | EAY86110.1 | 0 | 2 | 833 | 1 | 823 | hypothetical protein OsI_07480 [Oryza sativa Indica Group] |
RefSeq | NP_001047009.1 | 0 | 2 | 833 | 1 | 823 | Os02g0528200 [Oryza sativa (japonica cultivar-group)] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3amk_A | 0 | 157 | 831 | 13 | 690 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
PDB | 3aml_A | 0 | 157 | 831 | 13 | 690 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
PDB | 3vu2_B | 0 | 157 | 831 | 13 | 690 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
PDB | 3vu2_A | 0 | 157 | 831 | 13 | 690 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
PDB | 3k1d_A | 1.4013e-45 | 210 | 833 | 137 | 721 | A Chain A, Crystal Structure Of Glycogen Branching Enzyme Synonym: 1,4- Glucan:1,4-Alpha-D-Glucan 6-Glucosyl-Transferase From Mycob Tuberculosis H3 |
Metabolic Pathways | |||
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Pathway Name | Reaction | EC | Protein Name |
starch biosynthesis | RXN-7710 | EC-2.4.1.18 | 1,4-α-glucan branching enzyme |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
HO794536 | 682 | 151 | 832 | 0 |
HO777638 | 626 | 207 | 832 | 0 |
HO458123 | 395 | 440 | 834 | 0 |
HO458123 | 289 | 151 | 438 | 0 |
HO777638 | 47 | 160 | 206 | 0.00000007 |
Sequence Alignments (This image is cropped. Click for full image.) |
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