| Basic Information | |
|---|---|
| Species | Brachypodium distachyon |
| Cazyme ID | Bradi4g07847.1 |
| Family | GH32 |
| Protein Properties | Length: 575 Molecular Weight: 62109.5 Isoelectric Point: 5.5718 |
| Chromosome | Chromosome/Scaffold: 4 Start: 6758072 End: 6760706 |
| Description | Glycosyl hydrolases family 32 protein |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
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| Family | Start | End | Evalue |
| GH32 | 15 | 341 | 0 |
| HFQPAKNWQNGPMYHNGMYHLFYQYNPHGPTWDAGKLSWGHSVSGDLVNWAALDNALDPTSPFDANGCWSGSATVLPGGRPAILYTGIDADRVQVQNVAF ASNPSDPLLRDWHKPSCNPVIGIPADVTGNNFRDPTEPWRGSDGLWRVAVAAEVEGKGTLLVYRSADFLRWERNPGPPLHASSPAVPVLECPDLFPISMA AAAEQEGLDALMAAGGSSSGGVTHVLKLTDFAKEDHYMVGRYDELAGDTFAPAEPERGDDPGRWRRLDHGHLYASKSFFDARKKRRVLWAWVDENDGAAE AKGWAGIQAFPRAMWLDGDGKGLVQWP | |||
| Full Sequence |
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| Protein Sequence Length: 575 Download |
| MDGTKNPQHG RTAYHFQPAK NWQNGPMYHN GMYHLFYQYN PHGPTWDAGK LSWGHSVSGD 60 LVNWAALDNA LDPTSPFDAN GCWSGSATVL PGGRPAILYT GIDADRVQVQ NVAFASNPSD 120 PLLRDWHKPS CNPVIGIPAD VTGNNFRDPT EPWRGSDGLW RVAVAAEVEG KGTLLVYRSA 180 DFLRWERNPG PPLHASSPAV PVLECPDLFP ISMAAAAEQE GLDALMAAGG SSSGGVTHVL 240 KLTDFAKEDH YMVGRYDELA GDTFAPAEPE RGDDPGRWRR LDHGHLYASK SFFDARKKRR 300 VLWAWVDEND GAAEAKGWAG IQAFPRAMWL DGDGKGLVQW PVEEIETLRR KRDSGFGPEG 360 TEVGAGGKVE IGAGIQSSQA DVEVVFEIPS LEEAETLDDL EWALDDPQRL CAEKGAFVHG 420 GVGPFGLLVL ASGGLQEHTA VFFRVFRHQG KYKVLMCTDL TRSSTKAGVN KPCYGAFLDV 480 DVEKDRSISL RTLIDHTVVE SFGNGGRTCM TARVYPEHAA KGRSRTYVFN HGAGAVKVSK 540 LEAWELAAAA VNGAGGGGGS VAPDVATVWS DFVI* |
| Functional Domains Download unfiltered results here | ||||||||
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| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01322 | scrB_fam | 1.0e-37 | 10 | 350 | 365 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| COG1621 | SacC | 2.0e-57 | 4 | 522 | 534 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 3.0e-76 | 24 | 344 | 328 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 3.0e-109 | 15 | 341 | 340 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 2.0e-136 | 15 | 506 | 505 | + Glycosyl hydrolases family 32. | ||
| Annotations - NR Download unfiltered results here | |||||||
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| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| Swiss-Prot | A2YZ01 | 0 | 11 | 563 | 40 | 585 | INV7_ORYSI RecName: Full=Beta-fructofuranosidase, insoluble isoenzyme 7; AltName: Full=Sucrose hydrolase 7; AltName: Full=Invertase 7; AltName: Full=Cell wall beta-fructosidase 7; AltName: Full=OsCIN7; Flags: Precursor |
| GenBank | AAD38399.1 | 0 | 11 | 563 | 40 | 585 | AF155121_1 apoplastic invertase [Oryza sativa Indica Group] |
| DDBJ | BAD23559.1 | 0 | 11 | 563 | 40 | 584 | putative apoplastic invertase [Oryza sativa Japonica Group] |
| EMBL | CAH65976.1 | 0 | 1 | 554 | 4 | 546 | H1005F08.5 [Oryza sativa (indica cultivar-group)] |
| GenBank | EEC84182.1 | 0 | 11 | 563 | 39 | 584 | hypothetical protein OsI_30564 [Oryza sativa Indica Group] |
| Annotations - PDB Download unfiltered results here | |||||||
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| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2aey_A | 0 | 8 | 549 | 5 | 531 | B Chain B, Structural Basis Of Gibberellin(Ga3)-Induced Della Recognition By The Gibberellin Receptor |
| PDB | 2ade_A | 0 | 8 | 549 | 5 | 531 | B Chain B, Structural Basis Of Gibberellin(Ga3)-Induced Della Recognition By The Gibberellin Receptor |
| PDB | 2add_A | 0 | 8 | 549 | 5 | 531 | B Chain B, Structural Basis Of Gibberellin(Ga3)-Induced Della Recognition By The Gibberellin Receptor |
| PDB | 1st8_A | 0 | 8 | 549 | 5 | 531 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia From Cichorium Intybus |
| PDB | 2aez_A | 0 | 8 | 549 | 5 | 531 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia (E201q) From Cichorium Intybus In Complex With 1-Kestose |
| Metabolic Pathways | |||
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| Pathway Name | Reaction | EC | Protein Name |
| sucrose degradation III | RXN-1461 | EC-3.2.1.26 | β-fructofuranosidase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
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| Hit | Length | Start | End | EValue |
| EE591202 | 422 | 129 | 546 | 0 |
| EE591202 | 223 | 1 | 214 | 0 |
| CT842376 | 549 | 11 | 546 | 0 |
| EG398418 | 269 | 278 | 543 | 0 |
| HO170408 | 254 | 280 | 531 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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