| Basic Information | |
|---|---|
| Species | Brachypodium distachyon |
| Cazyme ID | Bradi5g08900.1 |
| Family | CE10 |
| Protein Properties | Length: 347 Molecular Weight: 37035.6 Isoelectric Point: 5.0321 |
| Chromosome | Chromosome/Scaffold: 5 Start: 12027655 End: 12028945 |
| Description | alpha/beta-Hydrolases superfamily protein |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| CE10 | 43 | 338 | 0 |
| EDRDDGRVEWRDAAYGGSRGLGVRMYRPKPPRERQGKKPLLRVLAYFHGGGFCIGSRAWPSVHACCLRLADEARAVVLSFDYRLAPEHRLPAAHEDAADA LLWLRDRLALTPNNDDDDGVGSWLADSGADPGRLFVSGESAGGNMAHHMAARFGDSGLGPVVKVAGYVLIMPAFTSEAPTESELESPATAALSRDVAERY NRLALPAGANKDHPLMNPLGPDSPGLARVGGRVLVVVGGEDMLKDNQLRYAEEMRAAGNDVELFVLPGEQHGFFSSKPWSEASGEVVRAIGRFMDR | |||
| Full Sequence |
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| Protein Sequence Length: 347 Download |
| MEPAVVEVEA TAPPHVVEDC RGVLQVLSDG TVVRSSASPY AVEDRDDGRV EWRDAAYGGS 60 RGLGVRMYRP KPPRERQGKK PLLRVLAYFH GGGFCIGSRA WPSVHACCLR LADEARAVVL 120 SFDYRLAPEH RLPAAHEDAA DALLWLRDRL ALTPNNDDDD GVGSWLADSG ADPGRLFVSG 180 ESAGGNMAHH MAARFGDSGL GPVVKVAGYV LIMPAFTSEA PTESELESPA TAALSRDVAE 240 RYNRLALPAG ANKDHPLMNP LGPDSPGLAR VGGRVLVVVG GEDMLKDNQL RYAEEMRAAG 300 NDVELFVLPG EQHGFFSSKP WSEASGEVVR AIGRFMDRDA AAAGSD* |
| Functional Domains Download unfiltered results here | ||||||
|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description |
| cd00312 | Esterase_lipase | 0.001 | 68 | 190 | 136 | + Esterases and lipases (includes fungal lipases, cholinesterases, etc.) These enzymes act on carboxylic esters (EC: 3.1.1.-). The catalytic apparatus involves three residues (catalytic triad): a serine, a glutamate or aspartate and a histidine.These catalytic residues are responsible for the nucleophilic attack on the carbonyl carbon atom of the ester bond. In contrast with other alpha/beta hydrolase fold family members, p-nitrobenzyl esterase and acetylcholine esterase have a Glu instead of Asp at the active site carboxylate. |
| COG2272 | PnbA | 0.0005 | 73 | 185 | 125 | + Carboxylesterase type B [Lipid metabolism] |
| PRK10162 | PRK10162 | 2.0e-8 | 65 | 315 | 262 | + acetyl esterase; Provisional |
| COG0657 | Aes | 9.0e-29 | 49 | 315 | 272 | + Esterase/lipase [Lipid metabolism] |
| pfam07859 | Abhydrolase_3 | 3.0e-55 | 88 | 317 | 234 | + alpha/beta hydrolase fold. This catalytic domain is found in a very wide range of enzymes. |
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0008152 | metabolic process |
| GO:0016787 | hydrolase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | EAZ02863.1 | 0 | 10 | 336 | 3 | 320 | hypothetical protein OsI_24993 [Oryza sativa Indica Group] |
| GenBank | EAZ02864.1 | 0 | 13 | 336 | 11 | 323 | hypothetical protein OsI_24995 [Oryza sativa Indica Group] |
| RefSeq | NP_001058947.1 | 0 | 12 | 336 | 5 | 320 | Os07g0162400 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | NP_001058948.1 | 0 | 13 | 336 | 11 | 323 | Os07g0162500 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | NP_001058951.1 | 0 | 16 | 339 | 7 | 321 | Os07g0162900 [Oryza sativa (japonica cultivar-group)] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2o7v_A | 4e-35 | 53 | 303 | 56 | 296 | A Chain A, Crystal Structure Of Medicago Truncatula Ugt71g1 Complexed With Udp-Glucose |
| PDB | 2o7r_A | 4e-35 | 53 | 303 | 56 | 296 | A Chain A, Plant Carboxylesterase Aecxe1 From Actinidia Eriantha With Acyl Adduct |
| PDB | 2zsi_A | 2e-34 | 66 | 316 | 86 | 329 | A Chain A, Plant Carboxylesterase Aecxe1 From Actinidia Eriantha With Acyl Adduct |
| PDB | 2zsh_A | 2e-34 | 66 | 316 | 86 | 329 | B Chain B, Structural Basis Of Gibberellin(Ga3)-Induced Della Recognition By The Gibberellin Receptor |
| PDB | 3ed1_F | 1e-33 | 60 | 337 | 72 | 348 | B Chain B, Structural Basis Of Gibberellin(Ga3)-Induced Della Recognition By The Gibberellin Receptor |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| formononetin biosynthesis | RXN-3284 | EC-4.2.1.105 | 2-hydroxyisoflavanone dehydratase |
| formononetin biosynthesis | RXN-3625 | - | 2,7-dihydroxy-4'-methoxyisoflavanone dehydratase |
| isoflavonoid biosynthesis I | RXN-3284 | EC-4.2.1.105 | 2-hydroxyisoflavanone dehydratase |
| isoflavonoid biosynthesis II | RXN-3303 | EC-4.2.1.105 | 2-hydroxyisoflavanone dehydratase |
| isoflavonoid biosynthesis II | RXN-5502 | - | 2,7,5-trihydroxy-4'-methoxyisoflavanone dehydratase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| GR365709 | 260 | 9 | 266 | 0 |
| GR331413 | 245 | 14 | 253 | 0 |
| DR735733 | 305 | 16 | 315 | 0 |
| CT857045 | 254 | 11 | 261 | 0 |
| GR365708 | 204 | 1 | 203 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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