Basic Information | |
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Species | Capsella rubella |
Cazyme ID | Carubv10002713m |
Family | GH32 |
Protein Properties | Length: 470 Molecular Weight: 51831.4 Isoelectric Point: 5.1293 |
Chromosome | Chromosome/Scaffold: 6 Start: 3812991 End: 3814890 |
Description | 6-&1-fructan exohydrolase |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH32 | 11 | 261 | 0 |
NWIQLEPAIVPSDPSDINSCWSGSATILPDGKPVMLYTGSDTNKHQVTDLAKPKDASDPLLREWVKAKGNPVMVPPSNVPRVLVGAKEKDSKKGMALLYH SDDFVQWTKSHVPLLESQVTGMWECVDFFPVSVTGREGLDTSVNNSSVRHVLKASYGGNDCYVIGSYSSETEIFSADSEFTNTADDLRYDYGNLFASKAF FDSATNRRISWGWIMERDSKEDDIVKGWAGIMGLPREIWLDRSGKKLMQWP |
Full Sequence |
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Protein Sequence Length: 470 Download |
MGTLCFTRFG NWIQLEPAIV PSDPSDINSC WSGSATILPD GKPVMLYTGS DTNKHQVTDL 60 AKPKDASDPL LREWVKAKGN PVMVPPSNVP RVLVGAKEKD SKKGMALLYH SDDFVQWTKS 120 HVPLLESQVT GMWECVDFFP VSVTGREGLD TSVNNSSVRH VLKASYGGND CYVIGSYSSE 180 TEIFSADSEF TNTADDLRYD YGNLFASKAF FDSATNRRIS WGWIMERDSK EDDIVKGWAG 240 IMGLPREIWL DRSGKKLMQW PVEEINNLRA KRVSLYNKQL GSGSVFEISG ITASQADIEV 300 AFDLRDLAND PEILDSEEVD QATLIASYSA SVRGVYGPFG LLALASNDLA EHTAIFFRVI 360 RRGDGHSVVM CSDETKSSLR DNIEKAAVGT VLDIDPRHEK ISLRCLIDHS VIESYGGEGR 420 SVITSRVYPK IAIGEESRLY VFNHGIKGVT MSSLEAWSMR KAQINSNAT* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
TIGR01322 | scrB_fam | 1.0e-29 | 11 | 430 | 460 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
COG1621 | SacC | 2.0e-32 | 11 | 429 | 454 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
cd08996 | GH32_B_Fructosidase | 3.0e-53 | 11 | 264 | 274 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam00251 | Glyco_hydro_32N | 1.0e-85 | 11 | 261 | 278 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
smart00640 | Glyco_32 | 7.0e-113 | 11 | 419 | 437 | + Glycosyl hydrolases family 32. |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
EMBL | CAB87665.1 | 0 | 11 | 469 | 69 | 546 | fructosidase-like protein [Arabidopsis thaliana] |
RefSeq | NP_568254.1 | 0 | 11 | 469 | 72 | 549 | AtcwINV6 (6-&1-fructan exohydrolase); hydrolase, hydrolyzing O-glycosyl compounds / inulinase/ levanase [Arabidopsis thaliana] |
RefSeq | XP_002278898.1 | 0 | 11 | 464 | 42 | 509 | PREDICTED: hypothetical protein isoform 2 [Vitis vinifera] |
RefSeq | XP_002278918.1 | 0 | 11 | 464 | 92 | 559 | PREDICTED: hypothetical protein isoform 3 [Vitis vinifera] |
RefSeq | XP_002309497.1 | 0 | 11 | 465 | 98 | 567 | predicted protein [Populus trichocarpa] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 2aey_A | 0 | 11 | 464 | 62 | 533 | A Chain A, The Structure Of Soybean Peroxidase |
PDB | 2ade_A | 0 | 11 | 464 | 62 | 533 | A Chain A, The Structure Of Soybean Peroxidase |
PDB | 2add_A | 0 | 11 | 464 | 62 | 533 | A Chain A, The Structure Of Soybean Peroxidase |
PDB | 1st8_A | 0 | 11 | 464 | 62 | 533 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia From Cichorium Intybus |
PDB | 2aez_A | 0 | 11 | 464 | 62 | 533 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia (E201q) From Cichorium Intybus In Complex With 1-Kestose |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
CT842376 | 485 | 11 | 465 | 0 |
DY944680 | 311 | 153 | 460 | 0 |
EX445967 | 295 | 169 | 463 | 0 |
DY925244 | 305 | 158 | 462 | 0 |
FE693031 | 280 | 182 | 461 | 0 |
Sequence Alignments (This image is cropped. Click for full image.) |
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