Basic Information | |
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Species | Capsella rubella |
Cazyme ID | Carubv10008995m |
Family | GH32 |
Protein Properties | Length: 484 Molecular Weight: 53293.3 Isoelectric Point: 5.1227 |
Chromosome | Chromosome/Scaffold: 1 Start: 4236552 End: 4240387 |
Description | Glycosyl hydrolases family 32 protein |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH32 | 3 | 264 | 0 |
ADQWYDSNGVWTGSATILPDGSIVMLYTGSTDKAVQVQNLAYPEDLNDPLLLKWVKYPGNPVLVPPPGILPKDFRDPTTAWKTSEGKWRITIGSKINKTG ISLVYDTTDFKTYEKLDTLLHGVPKTGMWECVDFYPVSKTESNGLDTSVNGPDVKHIVKASMDDTRFDHYAVGTYFESNGTWIPDDPTIDVGMSSSLRYD YGKFYASKTFYDQNKGRRILWSWIGESDSEASDVQKGWSSLQGIPRTVALDTKTGKNLVQWP |
Full Sequence |
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Protein Sequence Length: 484 Download |
MVADQWYDSN GVWTGSATIL PDGSIVMLYT GSTDKAVQVQ NLAYPEDLND PLLLKWVKYP 60 GNPVLVPPPG ILPKDFRDPT TAWKTSEGKW RITIGSKINK TGISLVYDTT DFKTYEKLDT 120 LLHGVPKTGM WECVDFYPVS KTESNGLDTS VNGPDVKHIV KASMDDTRFD HYAVGTYFES 180 NGTWIPDDPT IDVGMSSSLR YDYGKFYASK TFYDQNKGRR ILWSWIGESD SEASDVQKGW 240 SSLQGIPRTV ALDTKTGKNL VQWPVEEIKS LRLSSKKFDL EVSPGSVVPV DVGTATQLDI 300 EAEFEIKKES LEKILGDSSV VAEAEEFSCE KSGGSTVRGA LGPFGFSVLA TESLSEQTPV 360 YFYIAKGKDS KLKTFFCTDT SRSSVANDVI KPIYGSVVPV LKGEKLTMRI LVDHSIVEAF 420 GQGGRTCITS RVYPTTAIYG AAKLFLFNNA LDTTVTASFK VWQMNSAFIH PYSEEAVRAL 480 TRA* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
TIGR01322 | scrB_fam | 3.0e-30 | 4 | 434 | 447 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
COG1621 | SacC | 9.0e-39 | 4 | 435 | 443 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
cd08996 | GH32_B_Fructosidase | 1.0e-57 | 4 | 267 | 270 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam00251 | Glyco_hydro_32N | 6.0e-106 | 1 | 264 | 272 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
smart00640 | Glyco_32 | 1.0e-137 | 1 | 424 | 434 | + Glycosyl hydrolases family 32. |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | AAG36942.1 | 0 | 1 | 481 | 181 | 661 | AF274298_1 acid invertase [Brassica oleracea] |
GenBank | AAG36943.1 | 0 | 1 | 482 | 180 | 661 | AF274299_1 acid invertase [Brassica oleracea] |
EMBL | CAA61624.1 | 0 | 1 | 475 | 81 | 554 | beta-fructofuranosidase [Arabidopsis thaliana] |
EMBL | CAA66330.1 | 0 | 1 | 482 | 178 | 659 | beta-fructosidase [Arabidopsis thaliana] |
RefSeq | NP_563901.1 | 0 | 1 | 482 | 182 | 663 | ATBETAFRUCT4; beta-fructofuranosidase/ hydrolase, hydrolyzing O-glycosyl compounds [Arabidopsis thaliana] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3ugh_B | 0 | 1 | 472 | 80 | 544 | A Chain A, Arabidopsis Thaliana Peroxidase N |
PDB | 3ugh_A | 0 | 1 | 472 | 80 | 544 | A Chain A, Arabidopsis Thaliana Peroxidase N |
PDB | 3ugg_B | 0 | 1 | 472 | 80 | 544 | A Chain A, Arabidopsis Thaliana Peroxidase N |
PDB | 3ugg_A | 0 | 1 | 472 | 80 | 544 | A Chain A, Arabidopsis Thaliana Peroxidase N |
PDB | 3ugf_B | 0 | 1 | 472 | 80 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
EX122353 | 355 | 109 | 463 | 0 |
EG480172 | 283 | 198 | 480 | 0 |
EG526800 | 276 | 57 | 332 | 0 |
EX134303 | 278 | 24 | 301 | 0 |
EG509267 | 265 | 216 | 480 | 0 |
Sequence Alignments (This image is cropped. Click for full image.) |
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