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Basic Information | |
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Species | Capsella rubella |
Cazyme ID | Carubv10019944m |
Family | GH32 |
Protein Properties | Length: 641 Molecular Weight: 71563 Isoelectric Point: 5.9276 |
Chromosome | Chromosome/Scaffold: 2 Start: 961686 End: 965495 |
Description | Glycosyl hydrolases family 32 protein |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH32 | 110 | 427 | 0 |
HFQPENHWMNGPLFYKGWYHFFYQHNPNGTVWGDIVWGHAVSKDLIHWLHLPIAMVPDQWYDVNGVLTGSATFLDDGSIVMLYTGITDKFVQVQNLAYPE DPSDPLLLKWVKFSGNPILLPPLGIGAKDFRDPTTAWKTSAGKWRITIGSKINRTGVSLVYDTTDFKTYELLDTLLHQVSNTGEWECVDFYPVSKTKVIG LDTSVKGPDVKHVVKASMDDTRMDLYAIGTYVDANGTWFPDNPSIDVGISTGLRYDYGTYYASKTFYDGNKGRRILWGFTIESDSVYADVQKGWASVQAI PRTVVLDTKTGNNLILWP |
Full Sequence |
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Protein Sequence Length: 641 Download |
MHSSEALLPV TSQQDPLSKS RSDTIPDTSW RRHIKLHIAV FSSLILIALY VTHIVTHEGS 60 NDETATESRA SLAGVSDKSN ERFWRVFDGP KREAFPWNDT MLSWQRTTFH FQPENHWMNG 120 PLFYKGWYHF FYQHNPNGTV WGDIVWGHAV SKDLIHWLHL PIAMVPDQWY DVNGVLTGSA 180 TFLDDGSIVM LYTGITDKFV QVQNLAYPED PSDPLLLKWV KFSGNPILLP PLGIGAKDFR 240 DPTTAWKTSA GKWRITIGSK INRTGVSLVY DTTDFKTYEL LDTLLHQVSN TGEWECVDFY 300 PVSKTKVIGL DTSVKGPDVK HVVKASMDDT RMDLYAIGTY VDANGTWFPD NPSIDVGIST 360 GLRYDYGTYY ASKTFYDGNK GRRILWGFTI ESDSVYADVQ KGWASVQAIP RTVVLDTKTG 420 NNLILWPVEE IESLRLNSKK YHMTVGPGTV IPVDVGSASQ LDIEAEFTIM KDDLEIILGD 480 DPVVADGKDY SCETNGGSTV RGALGPFGFS VLADQTLSEH TPVYFYVTKG KDSKLKTFFC 540 TDNLRSSMAN DVVKSVYGSL VPVLKGEKLT MRILVDHSII EGFGQGGRTC ITSRAYPTKA 600 IYGATKLFLF NNAIDANVTA SFKVWQMNSA FIHPYSEDDL * 660 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
TIGR01322 | scrB_fam | 1.0e-41 | 89 | 597 | 530 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
COG1621 | SacC | 2.0e-59 | 106 | 626 | 540 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
cd08996 | GH32_B_Fructosidase | 4.0e-80 | 116 | 430 | 324 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam00251 | Glyco_hydro_32N | 2.0e-132 | 110 | 427 | 329 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
smart00640 | Glyco_32 | 9.0e-164 | 110 | 587 | 491 | + Glycosyl hydrolases family 32. |
Gene Ontology | |
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GO Term | Description |
GO:0004564 | beta-fructofuranosidase activity |
GO:0004575 | sucrose alpha-glucosidase activity |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | AAD43622.1 | 0 | 1 | 640 | 1 | 644 | AC005698_21 T3P18.21 [Arabidopsis thaliana] |
GenBank | AAF19535.1 | 0 | 1 | 640 | 1 | 644 | AC007190_3 F23N19.3 [Arabidopsis thaliana] |
GenBank | AAK62665.1 | 0 | 1 | 640 | 1 | 642 | At1g62660/F23N19_3 [Arabidopsis thaliana] |
EMBL | CAA64781.1 | 0 | 10 | 640 | 1 | 633 | beta-fructosidase [Arabidopsis thaliana] |
RefSeq | NP_564798.1 | 0 | 1 | 640 | 1 | 642 | beta-fructosidase (BFRUCT3) / beta-fructofuranosidase / invertase, vacuolar [Arabidopsis thaliana] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3ugh_B | 0 | 95 | 635 | 8 | 544 | B Chain B, The Avrptob-Bak1 Complex Reveals Two Structurally Similar Kinaseinteracting Domains In A Single Type Iii Effector |
PDB | 3ugh_A | 0 | 95 | 635 | 8 | 544 | B Chain B, The Avrptob-Bak1 Complex Reveals Two Structurally Similar Kinaseinteracting Domains In A Single Type Iii Effector |
PDB | 3ugg_B | 0 | 95 | 635 | 8 | 544 | B Chain B, The Avrptob-Bak1 Complex Reveals Two Structurally Similar Kinaseinteracting Domains In A Single Type Iii Effector |
PDB | 3ugg_A | 0 | 95 | 635 | 8 | 544 | B Chain B, The Avrptob-Bak1 Complex Reveals Two Structurally Similar Kinaseinteracting Domains In A Single Type Iii Effector |
PDB | 3ugf_B | 0 | 95 | 635 | 8 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
EX122353 | 360 | 272 | 626 | 0 |
EX134303 | 278 | 187 | 464 | 0 |
EG480171 | 280 | 83 | 359 | 0 |
GR451225 | 269 | 181 | 449 | 0 |
FY440918 | 275 | 309 | 583 | 0 |
Sequence Alignments (This image is cropped. Click for full image.) |
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