Basic Information | |
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Species | Citrus clementina |
Cazyme ID | Ciclev10004465m |
Family | GH32 |
Protein Properties | Length: 686 Molecular Weight: 76087 Isoelectric Point: 6.1746 |
Chromosome | Chromosome/Scaffold: 9 Start: 27282487 End: 27287560 |
Description | Glycosyl hydrolases family 32 protein |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH32 | 165 | 483 | 0 |
HFQPEKNWMNDPNGPLFYKGWYHLFYQYNPDSAVWGNITWGHAVSADLIHWLYLPIAMVPDQWYDINGVWTGSATILPDGQIVMLYTGSTDKSVQVQNLA YPADPSDPLLLDWVKYPGNPVLVPPRHIGPKDFRDPTTAWAGPDGKWRLTIGSKIGKTGISLVYQTTDFKTYELLDEYLHAVPGTGMWECVDFYPVAING SVGLDTSATGPGIKHVLKASLDDTKVDHYAIGTYNPENDKWTPDNPEEDVGIGLKWDYGRYYASKSFYDPYKKRRIVWGWINETDTESDDLEKGWASVQT IPRTVLYDNKTGSNVVQWP |
Full Sequence |
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Protein Sequence Length: 686 Download |
MAVVTKLPLS SLALSFHLSN SFYLNPISPF SLLFSFSFST HPKMDTPYHH LHAPPHYPPL 60 LPQEQPSDAG PPASHRKPFK GFAAILASVI FLLSLVALII NQTQKPLPSQ NNIVPTSKPT 120 SFSNPEPRGV AEGVSAKSNS HLLRNIKGSY NWTNAMFTWQ RTSFHFQPEK NWMNDPNGPL 180 FYKGWYHLFY QYNPDSAVWG NITWGHAVSA DLIHWLYLPI AMVPDQWYDI NGVWTGSATI 240 LPDGQIVMLY TGSTDKSVQV QNLAYPADPS DPLLLDWVKY PGNPVLVPPR HIGPKDFRDP 300 TTAWAGPDGK WRLTIGSKIG KTGISLVYQT TDFKTYELLD EYLHAVPGTG MWECVDFYPV 360 AINGSVGLDT SATGPGIKHV LKASLDDTKV DHYAIGTYNP ENDKWTPDNP EEDVGIGLKW 420 DYGRYYASKS FYDPYKKRRI VWGWINETDT ESDDLEKGWA SVQTIPRTVL YDNKTGSNVV 480 QWPVEEIESL RQNSTVFEEV VVEPGSVVPL DIGVATQLDI SAEFETELLG SGATEEGYGC 540 SGGAVDRSAM GPFGLLVNAH DSLSELTPIF FRSSNTTNGT NTYFCTDETR SSLAPDVFKQ 600 VHGSKVPVLQ GEKLSMRILV DHSIVESFGQ GGRTVITSRI YPTKAIYGAA RLFLFNNATG 660 VNVKATLKIW RLNSAFIHPF PLDQI* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
TIGR01322 | scrB_fam | 4.0e-54 | 161 | 642 | 503 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
COG1621 | SacC | 1.0e-74 | 161 | 643 | 498 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
cd08996 | GH32_B_Fructosidase | 3.0e-95 | 171 | 486 | 328 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam00251 | Glyco_hydro_32N | 4.0e-152 | 165 | 483 | 327 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
smart00640 | Glyco_32 | 0 | 165 | 632 | 477 | + Glycosyl hydrolases family 32. |
Gene Ontology | |
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GO Term | Description |
GO:0004564 | beta-fructofuranosidase activity |
GO:0004575 | sucrose alpha-glucosidase activity |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | AAL27709.3 | 0 | 44 | 685 | 1 | 588 | AF433643_1 vacuolar invertase [Citrus sinensis] |
GenBank | ACJ04702.1 | 0 | 44 | 680 | 1 | 629 | invertase 2 [Cucumis melo] |
DDBJ | BAF34363.1 | 0 | 44 | 685 | 1 | 642 | beta-fructofuranosidase [Citrus sinensis] |
DDBJ | BAG30919.1 | 0 | 44 | 685 | 1 | 645 | soluble acid invertase [Pyrus pyrifolia] |
RefSeq | XP_002510944.1 | 0 | 52 | 684 | 11 | 638 | Beta-fructofuranosidase, soluble isoenzyme I precursor, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3ugh_B | 0 | 150 | 680 | 8 | 544 | A Chain A, 3.0 A Structure Of The Prunus Mume Hydroxynitrile Lyase Isozyme-1 |
PDB | 3ugh_A | 0 | 150 | 680 | 8 | 544 | A Chain A, 3.0 A Structure Of The Prunus Mume Hydroxynitrile Lyase Isozyme-1 |
PDB | 3ugg_B | 0 | 150 | 680 | 8 | 544 | A Chain A, 3.0 A Structure Of The Prunus Mume Hydroxynitrile Lyase Isozyme-1 |
PDB | 3ugg_A | 0 | 150 | 680 | 8 | 544 | A Chain A, 3.0 A Structure Of The Prunus Mume Hydroxynitrile Lyase Isozyme-1 |
PDB | 3ugf_B | 0 | 150 | 680 | 8 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |