Basic Information | |
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Species | Citrus clementina |
Cazyme ID | Ciclev10007401m |
Family | GH13 |
Protein Properties | Length: 902 Molecular Weight: 103971 Isoelectric Point: 6.5829 |
Chromosome | Chromosome/Scaffold: 1 Start: 22702187 End: 22710340 |
Description | Alpha amylase family protein |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH13 | 419 | 744 | 3.9e-23 |
FNEFTEKVLPHVKEAGYNVIQLFGVVEHKDYFTVGYRVTNLYAVSSRYGTPDDFKRLVDEAHGLGLLVFLDIVHSYSAADQMVGLSQFDGSNDCYFHTGK RGFHKYWGTRMFKYDDLDVLHFLLSNLNWWVVEYQIDGFQFHSLSSMIYTHNGFASLTGDLEEYCNQYVDKDALLYLILANEILHALHPNIITIAEDATY YPGLCEPTTQGGLGFDYFLNLSASEMWLSFLENTPDHEWSMSKIVSTLVGNGQYSDKMIMYAENHNQSISGGQSFAEILFGEISEHSPDTNNLLLRGCSL HKMIRLITFTIGGHAYLNFMGNEFGH |
Full Sequence |
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Protein Sequence Length: 902 Download |
MTSLSLKTKI SLNPNNSALH FNVPNKLQHT NFPKKGKIKI KVTCSATDQP PQQQQQQTYS 60 KKKRNASEGD KGIDPVGFLN KVGITHKPLA QFLRERHKEL KNRKDEIFKR FLNLMEFSTG 120 YEIVGMHRNV EHRVDFMDWA PGARYCALVG DFNGWSPTEN CAREGHLGHD DYGYWFIILE 180 DKLREGEKPD ELYFQQYNYV DDYDKGDSGV SIQEIFKRAN DEYWEPGEDR FVKNRFELPA 240 KLYEQLFGPN GPQTLQELEE MPDAQTTYKA WKEQHKDDLP SNLPSYDVID NGKDYDVFNV 300 ASDPRWQEKF RSKEPPIPYW LETRKGRKAW LKKYTPGIPH GSKYRVYFNT PDGPLERIPA 360 WATYVQPDAD GKEAFAIHWE PSPEFAYKWR NTRPKVPKSL RIYECHVGIS GSKPKISSFN 420 EFTEKVLPHV KEAGYNVIQL FGVVEHKDYF TVGYRVTNLY AVSSRYGTPD DFKRLVDEAH 480 GLGLLVFLDI VHSYSAADQM VGLSQFDGSN DCYFHTGKRG FHKYWGTRMF KYDDLDVLHF 540 LLSNLNWWVV EYQIDGFQFH SLSSMIYTHN GFASLTGDLE EYCNQYVDKD ALLYLILANE 600 ILHALHPNII TIAEDATYYP GLCEPTTQGG LGFDYFLNLS ASEMWLSFLE NTPDHEWSMS 660 KIVSTLVGNG QYSDKMIMYA ENHNQSISGG QSFAEILFGE ISEHSPDTNN LLLRGCSLHK 720 MIRLITFTIG GHAYLNFMGN EFGHPKRVEF PMPSNNFSFS LANRHWDLLA NRLHSNLYSF 780 DQELMKLDEN AKVLLRGSPS VHHVNDAKMV ICYMRGPLVF IFNFHPTDSY EDYSVGVEEA 840 GEYQIILNTD ESKFGGQGLI KEHQYLQRTI SKRVDGLRNC IEVPLPSRTA QVYKLSRILR 900 I* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
PLN02447 | PLN02447 | 4.0e-14 | 87 | 181 | 95 | + 1,4-alpha-glucan-branching enzyme | ||
cd11321 | AmyAc_bac_euk_BE | 9.0e-173 | 383 | 782 | 411 | + Alpha amylase catalytic domain found in bacterial and eukaryotic branching enzymes. Branching enzymes (BEs) catalyze the formation of alpha-1,6 branch points in either glycogen or starch by cleavage of the alpha-1,4 glucosidic linkage yielding a non-reducing end oligosaccharide chain, and subsequent attachment to the alpha-1,6 position. By increasing the number of non-reducing ends, glycogen is more reactive to synthesis and digestion as well as being more soluble. This group includes bacterial and eukaryotic proteins. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
PLN02960 | PLN02960 | 0 | 1 | 901 | 904 | + alpha-amylase | ||
PLN03244 | PLN03244 | 0 | 1 | 901 | 908 | + alpha-amylase; Provisional | ||
PLN02447 | PLN02447 | 0 | 335 | 893 | 570 | + 1,4-alpha-glucan-branching enzyme |
Gene Ontology | |
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GO Term | Description |
GO:0003824 | catalytic activity |
GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
GO:0005975 | carbohydrate metabolic process |
GO:0043169 | cation binding |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
DDBJ | BAB02827.1 | 0 | 1 | 901 | 1 | 903 | starch-branching enzyme-like protein [Arabidopsis thaliana] |
EMBL | CBI26672.1 | 0 | 1 | 901 | 1 | 896 | unnamed protein product [Vitis vinifera] |
RefSeq | NP_001154629.1 | 0 | 1 | 901 | 1 | 899 | alpha-amylase/ catalytic/ cation binding [Arabidopsis thaliana] |
RefSeq | XP_002278858.1 | 0 | 1 | 901 | 1 | 866 | PREDICTED: hypothetical protein [Vitis vinifera] |
RefSeq | XP_002529457.1 | 0 | 1 | 892 | 1 | 892 | 1,4-alpha-glucan branching enzyme, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3amk_A | 0 | 336 | 860 | 114 | 650 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
PDB | 3vu2_B | 0 | 336 | 860 | 114 | 650 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
PDB | 3vu2_A | 0 | 336 | 860 | 114 | 650 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
BF272517 | 278 | 357 | 634 | 0 |
JG636329 | 270 | 344 | 613 | 0 |
CO104525 | 273 | 384 | 656 | 0 |
DY965821 | 292 | 266 | 557 | 0 |
EY851016 | 233 | 661 | 893 | 0 |
Sequence Alignments (This image is cropped. Click for full image.) |
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