Basic Information | |
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Species | Citrus clementina |
Cazyme ID | Ciclev10010769m |
Family | GH18 |
Protein Properties | Length: 589 Molecular Weight: 66446 Isoelectric Point: 7.6653 |
Chromosome | Chromosome/Scaffold: 1 Start: 28651777 End: 28654413 |
Description | Glycosyl hydrolase family protein with chitinase insertion domain |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH18 | 41 | 314 | 2.6e-31 |
PIAEIHSALFSHLMCAFAFINSSTYNIFINSTSEQFFVIFTNTVKHRNPSVVTLLSIWGGAIFSSMINQSSNRKSFIKSSVEMARFNGFHGLDLHGVLPD KGTNITKLGTLFDEWRAESQLLLVMTSHHLPALESVSYPLDSMQRNLDWIHVLNFDYYLPTRDNFTEAVLGLPYHGYAWTLVNPDENPVGSPATGPAITI DGSVGFKFIKGFIRDYGYGAASVYNHSYVMNFFSAKTTWVNFDGVETIRSKVSFAKEKGLLGYHAFQLSNDDKW |
Full Sequence |
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Protein Sequence Length: 589 Download |
MAYKIVTLFL LLTLSLPLNL QCTRSPNWIR GGYWHAHSEL PIAEIHSALF SHLMCAFAFI 60 NSSTYNIFIN STSEQFFVIF TNTVKHRNPS VVTLLSIWGG AIFSSMINQS SNRKSFIKSS 120 VEMARFNGFH GLDLHGVLPD KGTNITKLGT LFDEWRAESQ LLLVMTSHHL PALESVSYPL 180 DSMQRNLDWI HVLNFDYYLP TRDNFTEAVL GLPYHGYAWT LVNPDENPVG SPATGPAITI 240 DGSVGFKFIK GFIRDYGYGA ASVYNHSYVM NFFSAKTTWV NFDGVETIRS KVSFAKEKGL 300 LGYHAFQLSN DDKWELYSAA QEVGNDQKNS PRMLGILAKG QEIAVKRLSK TSQQGLQEFE 360 NEVKLTARLQ HVNLVPVVGI YPHRRHLLDW RQRVHIIEGV TQALLYVQEY SNFTIVRRDL 420 KASNILLDKE MKPKISDFGM ARAFTKDACE ANTGRIVGTY YGVLPLQIIS GKRTSRYYGR 480 NEDLNLLEFA YELRKNGEGT EFFDPSLDDS SSAWKLMRCM QIALLCVQEN AADRPAVLEI 540 LTMLNNETAN IKVPRKPAFS VKSDEDECIP EVKICSVNDA SITQLIPR* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
cd00598 | GH18_chitinase-like | 4.0e-19 | 34 | 215 | 189 | + The GH18 (glycosyl hydrolase, family 18) type II chitinases hydrolyze chitin, an abundant polymer of beta-1,4-linked N-acetylglucosamine (GlcNAc) which is a major component of the cell wall of fungi and the exoskeleton of arthropods. Chitinases have been identified in viruses, bacteria, fungi, protozoan parasites, insects, and plants. The structure of the GH18 domain is an eight-stranded beta/alpha barrel with a pronounced active-site cleft at the C-terminal end of the beta-barrel. The GH18 family includes chitotriosidase, chitobiase, hevamine, zymocin-alpha, narbonin, SI-CLP (stabilin-1 interacting chitinase-like protein), IDGF (imaginal disc growth factor), CFLE (cortical fragment-lytic enzyme) spore hydrolase, the type III and type V plant chitinases, the endo-beta-N-acetylglucosaminidases, and the chitolectins. The GH85 (glycosyl hydrolase, family 85) ENGases (endo-beta-N-acetylglucosaminidases) are closely related to the GH18 chitinases and are included in this alignment model. | ||
cd02872 | GH18_chitolectin_chitotriosidase | 2.0e-25 | 49 | 312 | 327 | + This conserved domain family includes a large number of catalytically inactive chitinase-like lectins (chitolectins) including YKL-39, YKL-40 (HCGP39), YM1, oviductin, and AMCase (acidic mammalian chitinase), as well as catalytically active chitotriosidases. The conserved domain is an eight-stranded alpha/beta barrel fold belonging to the family 18 glycosyl hydrolases. The fold has a pronounced active-site cleft at the C-terminal end of the beta-barrel. The chitolectins lack a key active site glutamate (the proton donor required for hydrolytic activity) but retain highly conserved residues involved in oligosaccharide binding. Chitotriosidase is a chitinolytic enzyme expressed in maturing macrophages, which suggests that it plays a part in antimicrobial defense. Chitotriosidase hydrolyzes chitotriose, as well as colloidal chitin to yield chitobiose and is therefore considered an exochitinase. Chitotriosidase occurs in two major forms, the large form being converted to the small form by either RNA or post-translational processing. Although the small form, containing the chitinase domain alone, is sufficient for the chitinolytic activity, the additional C-terminal chitin-binding domain of the large form plays a role in processing colloidal chitin. The chitotriosidase gene is nonessential in humans, as about 35% of the population are heterozygous and 6% homozygous for an inactivated form of the gene. HCGP39 is a 39-kDa human cartilage glycoprotein thought to play a role in connective tissue remodeling and defense against pathogens. | ||
pfam00704 | Glyco_hydro_18 | 1.0e-37 | 31 | 311 | 326 | + Glycosyl hydrolases family 18. | ||
smart00636 | Glyco_18 | 7.0e-41 | 41 | 311 | 327 | + Glyco_18 domain. | ||
cd02879 | GH18_plant_chitinase_class_V | 1.0e-84 | 26 | 316 | 340 | + The class V plant chitinases have a glycosyl hydrolase family 18 (GH18) domain, but lack the chitin-binding domain present in other GH18 enzymes. The GH18 domain of the class V chitinases has endochitinase activity in some cases and no catalytic activity in others. Included in this family is a lectin found in black locust (Robinia pseudoacacia) bark, which binds chitin but lacks chitinase activity. Also included is a chitinase-related receptor-like kinase (CHRK1) from tobacco (Nicotiana tabacum), with an N-terminal GH18 domain and a C-terminal kinase domain, which is thought to be part of a plant signaling pathway. The GH18 domain of CHRK1 is expressed extracellularly where it binds chitin but lacks chitinase activity. |
Gene Ontology | |
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GO Term | Description |
GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
GO:0004672 | protein kinase activity |
GO:0005524 | ATP binding |
GO:0005975 | carbohydrate metabolic process |
GO:0006468 | protein phosphorylation |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
EMBL | CBI35714.1 | 0 | 1 | 329 | 396 | 732 | unnamed protein product [Vitis vinifera] |
RefSeq | XP_002263792.1 | 0 | 1 | 329 | 1 | 367 | PREDICTED: hypothetical protein [Vitis vinifera] |
RefSeq | XP_002263792.1 | 0 | 331 | 588 | 446 | 753 | PREDICTED: hypothetical protein [Vitis vinifera] |
RefSeq | XP_002324560.1 | 0 | 7 | 316 | 6 | 370 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002523520.1 | 0 | 28 | 588 | 5 | 721 | conserved hypothetical protein [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3aqu_D | 0 | 29 | 334 | 5 | 353 | A Chain A, Crystal Structure Of A Class V Chitinase From Arabidopsis Thaliana |
PDB | 3aqu_C | 0 | 29 | 334 | 5 | 353 | A Chain A, Crystal Structure Of A Class V Chitinase From Arabidopsis Thaliana |
PDB | 3aqu_B | 0 | 29 | 334 | 5 | 353 | A Chain A, Crystal Structure Of A Class V Chitinase From Arabidopsis Thaliana |
PDB | 3aqu_A | 0 | 29 | 334 | 5 | 353 | A Chain A, Crystal Structure Of A Class V Chitinase From Arabidopsis Thaliana |
PDB | 3alg_A | 0 | 29 | 323 | 4 | 344 | A Chain A, Crystal Structure Of Class V Chitinase (E115q Mutant) From Nicotiana Tobaccum In Complex With Nag4 |