| Basic Information | |
|---|---|
| Species | Citrus clementina |
| Cazyme ID | Ciclev10019134m |
| Family | GH32 |
| Protein Properties | Length: 688 Molecular Weight: 76398.8 Isoelectric Point: 6.0845 |
| Chromosome | Chromosome/Scaffold: 3 Start: 5981066 End: 5985524 |
| Description | Glycosyl hydrolases family 32 protein |
| View CDS | |
| External Links |
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| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
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| Family | Start | End | Evalue |
| GH32 | 123 | 441 | 0 |
| HFQPQKNWMNDPNGPVFYKGWYHLFYQYNPNGAIWGDIVWGHAVSKDLIHWYHLPLAMVADQWYDIMGVWTGSATILPDGKLMMLYTGSTNESVQVQNLA YPADPSDPLLIKWVKYPGNPVLVPPPGIGAKDFRDPTTAWLTSEGKWRIAIGSRINRTGITFVYDTKDFINYELLRGVLHGVPNTGMWECVDFYPVSTTG EHGLDTSHNGPGVKHVVKASMDDDRHDYYAIGTYHEKNVTWVPDNPEIDVGIGIRYDYGLFYASKTFYDQNKKRRVLWGWIGESDSEIADVKKGWASLQG IPRTVALDTKTGSNLLQWP | |||
| Full Sequence |
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| Protein Sequence Length: 688 Download |
| MADSNVSYNP LPDGERPRPT RRRSIVLVLS GLLVVGFLVA LLSDNGSIAP TNVIDNDNNN 60 VNDNAQNGLN DVSSSSKDPE RLRPFTSASS AGVSEKSNRR FGARTASYPW TNNMLSWQRT 120 AFHFQPQKNW MNDPNGPVFY KGWYHLFYQY NPNGAIWGDI VWGHAVSKDL IHWYHLPLAM 180 VADQWYDIMG VWTGSATILP DGKLMMLYTG STNESVQVQN LAYPADPSDP LLIKWVKYPG 240 NPVLVPPPGI GAKDFRDPTT AWLTSEGKWR IAIGSRINRT GITFVYDTKD FINYELLRGV 300 LHGVPNTGMW ECVDFYPVST TGEHGLDTSH NGPGVKHVVK ASMDDDRHDY YAIGTYHEKN 360 VTWVPDNPEI DVGIGIRYDY GLFYASKTFY DQNKKRRVLW GWIGESDSEI ADVKKGWASL 420 QGIPRTVALD TKTGSNLLQW PVEEVDSLRL TSKEFKKIEL KPGSVMPLDV GSATQLDIVA 480 EFELDKAALE KTAESNVEFS CSSSEGSAER GALGPFGLLV LADDSLSEQT PVYFYIAKGK 540 DGSLKTYFCT DQSRSSEAND VNKSKYGSFV PVLEGEKFSM RVLVDHSIVE GFAQGGRTTI 600 TSRVYPTKAI YGAARVFLFN NATGASVTSS LKAWQMNSAF IRPYPDDDDE RHSGTEPTYE 660 VRGSKTKYLG NLLTSSLLFL NARNNFL* |
| Functional Domains Download unfiltered results here | ||||||||
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| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01322 | scrB_fam | 2.0e-56 | 119 | 606 | 504 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| COG1621 | SacC | 1.0e-71 | 119 | 607 | 499 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 2.0e-97 | 129 | 444 | 322 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 2.0e-155 | 123 | 441 | 327 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 0 | 123 | 596 | 484 | + Glycosyl hydrolases family 32. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004564 | beta-fructofuranosidase activity |
| GO:0004575 | sucrose alpha-glucosidase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| DDBJ | BAB82419.1 | 0 | 1 | 687 | 1 | 687 | acid invertase [Citrus unshiu] |
| DDBJ | BAF34362.1 | 0 | 1 | 687 | 1 | 687 | beta-fructofuranosidase [Citrus sinensis] |
| Swiss-Prot | P29001 | 0 | 11 | 651 | 8 | 648 | INVA_PHAAU RecName: Full=Acid beta-fructofuranosidase; AltName: Full=Acid sucrose hydrolase; AltName: Full=Acid invertase; Short=AI; AltName: Full=Vacuolar invertase; Contains: RecName: Full=Acid beta-fructofuranosidase 30 kDa subunit; Contains: RecName: Full=Acid beta-fructofuranosidase 38 kDa subunit; Flags: Precursor |
| RefSeq | XP_002303519.1 | 0 | 79 | 647 | 89 | 660 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002532576.1 | 0 | 72 | 646 | 72 | 647 | Acid beta-fructofuranosidase precursor, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3ugh_B | 0 | 108 | 644 | 8 | 544 | A Chain A, The Structure Of Endoglucanase From Termite, Nasutitermes Takasagoensis, At Ph 2.5. |
| PDB | 3ugh_A | 0 | 108 | 644 | 8 | 544 | A Chain A, The Structure Of Endoglucanase From Termite, Nasutitermes Takasagoensis, At Ph 2.5. |
| PDB | 3ugg_B | 0 | 108 | 644 | 8 | 544 | A Chain A, The Structure Of Endoglucanase From Termite, Nasutitermes Takasagoensis, At Ph 2.5. |
| PDB | 3ugg_A | 0 | 108 | 644 | 8 | 544 | A Chain A, The Structure Of Endoglucanase From Termite, Nasutitermes Takasagoensis, At Ph 2.5. |
| PDB | 3ugf_B | 0 | 108 | 644 | 8 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
