Basic Information | |
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Species | Citrus clementina |
Cazyme ID | Ciclev10019787m |
Family | GH32 |
Protein Properties | Length: 509 Molecular Weight: 56269.3 Isoelectric Point: 5.139 |
Chromosome | Chromosome/Scaffold: 3 Start: 5981066 End: 5985524 |
Description | Glycosyl hydrolases family 32 protein |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH32 | 3 | 262 | 0 |
ADQWYDIMGVWTGSATILPDGKLMMLYTGSTNESVQVQNLAYPADPSDPLLIKWVKYPGNPVLVPPPGIGAKDFRDPTTAWLTSEGKWRIAIGSRINRTG ITFVYDTKDFINYELLRGVLHGVPNTGMWECVDFYPVSTTGEHGLDTSHNGPGVKHVVKASMDDDRHDYYAIGTYHEKNVTWVPDNPEIDVGIGIRYDYG LFYASKTFYDQNKKRRVLWGWIGESDSEIADVKKGWASLQGIPRTVALDTKTGSNLLQWP |
Full Sequence |
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Protein Sequence Length: 509 Download |
MVADQWYDIM GVWTGSATIL PDGKLMMLYT GSTNESVQVQ NLAYPADPSD PLLIKWVKYP 60 GNPVLVPPPG IGAKDFRDPT TAWLTSEGKW RIAIGSRINR TGITFVYDTK DFINYELLRG 120 VLHGVPNTGM WECVDFYPVS TTGEHGLDTS HNGPGVKHVV KASMDDDRHD YYAIGTYHEK 180 NVTWVPDNPE IDVGIGIRYD YGLFYASKTF YDQNKKRRVL WGWIGESDSE IADVKKGWAS 240 LQGIPRTVAL DTKTGSNLLQ WPVEEVDSLR LTSKEFKKIE LKPGSVMPLD VGSATQLDIV 300 AEFELDKAAL EKTAESNVEF SCSSSEGSAE RGALGPFGLL VLADDSLSEQ TPVYFYIAKG 360 KDGSLKTYFC TDQSRSSEAN DVNKSKYGSF VPVLEGEKFS MRVLVDHSIV EGFAQGGRTT 420 ITSRVYPTKA IYGAARVFLF NNATGASVTS SLKAWQMNSA FIRPYPDDDD ERHSGTEPTY 480 EVRGSKTKYL GNLLTSSLLF LNARNNFL* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
TIGR01322 | scrB_fam | 8.0e-27 | 4 | 427 | 440 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
COG1621 | SacC | 4.0e-36 | 4 | 428 | 443 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
cd08996 | GH32_B_Fructosidase | 5.0e-57 | 4 | 265 | 268 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam00251 | Glyco_hydro_32N | 1.0e-105 | 1 | 262 | 270 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
smart00640 | Glyco_32 | 4.0e-145 | 1 | 417 | 427 | + Glycosyl hydrolases family 32. |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | ACT21538.1 | 0 | 1 | 472 | 107 | 578 | acid invertase [Vigna radiata] |
DDBJ | BAB82419.1 | 0 | 1 | 508 | 180 | 687 | acid invertase [Citrus unshiu] |
DDBJ | BAF34362.1 | 0 | 1 | 508 | 180 | 687 | beta-fructofuranosidase [Citrus sinensis] |
RefSeq | XP_002303519.1 | 0 | 1 | 468 | 193 | 660 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002532576.1 | 0 | 1 | 467 | 181 | 647 | Acid beta-fructofuranosidase precursor, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3ugh_B | 0 | 1 | 465 | 80 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
PDB | 3ugh_A | 0 | 1 | 465 | 80 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
PDB | 3ugg_B | 0 | 1 | 465 | 80 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
PDB | 3ugg_A | 0 | 1 | 465 | 80 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
PDB | 3ugf_B | 0 | 1 | 465 | 80 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
FC925873 | 277 | 159 | 435 | 0 |
DC895798 | 277 | 213 | 489 | 0 |
EY817233 | 275 | 38 | 312 | 0 |
EY714434 | 284 | 202 | 485 | 0 |
DY262106 | 263 | 159 | 421 | 0 |
Sequence Alignments (This image is cropped. Click for full image.) |
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