y
Basic Information | |
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Species | Citrus clementina |
Cazyme ID | Ciclev10033422m |
Family | GH31 |
Protein Properties | Length: 916 Molecular Weight: 102187 Isoelectric Point: 6.9685 |
Chromosome | Chromosome/Scaffold: 4 Start: 19340763 End: 19345467 |
Description | alpha-xylosidase 1 |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH31 | 298 | 790 | 0 |
FYFFAGPSPLAVVDQYTAFIGRPAPMPYWSLGFHQCRWGYHNLSVVEDVVENYKKAKIPLDVIWNDDDHMDGHKDFTLNPTNYPRPKLLAFLEKIHKIGM KYIVIIDPGIGVNSSYGVYQRGIANDVFIKYEGEPYLAQVWPGAVNFPDFLNPKTVSWWGDEIRRFHELVPVDGLWIDMNEASNFCSGLCKIPKGKQCPT GTGPGWVCCLDCKNITKTRWDDPPYKINASGLQVPIGFKTIATSAYHYNGVLEYDAHSIYGFSQSIATHKALLGLEGKRPFILSRSTFVGSGHYAAHWTG DNKGTWEDLKYSISTMLNFGIFGVPMVGSDICGFYPAPTEELCNRWIEVGAFYPFSRDHANYYSPRQELYQWESVAESARNALGMRYKLLPFLYTLNYEA HLSGAPIARPLFFSFPNYVECYNVSTQFLLGSSLMVSPVLEQGKSQVKALFPPGSWYNVFDMTQAISSKDGKFVTLDAPLHVVNVHLYQNTIL |
Full Sequence |
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Protein Sequence Length: 916 Download |
MPIIITCVPK KGVHPPHMYL NSNERRISHI TRSQFTLSLC QCVYIYIKSF SAHTVSVASI 60 GKGYRLISIE EVDGGILGHL QVKEKNNIYG PDIPLLQLYV KHETEDRLRV HITDAQKQRW 120 EVPYNLLPRE QPPKLKQTIG RTRKNPIAVS EYSSNGLIFS YSADPFSFAV KRKSNGETLF 180 NTSSDESDPF GPMVFKDQYL EISTKLPKDA SLYGLGENTQ PHGIKLYPND PYTLYTTDVS 240 AINLNTDLYG SHPVYMDLRN VNGEGAAHGV LLLSSNGMDV FYTGTSLTYK IIGGVFDFYF 300 FAGPSPLAVV DQYTAFIGRP APMPYWSLGF HQCRWGYHNL SVVEDVVENY KKAKIPLDVI 360 WNDDDHMDGH KDFTLNPTNY PRPKLLAFLE KIHKIGMKYI VIIDPGIGVN SSYGVYQRGI 420 ANDVFIKYEG EPYLAQVWPG AVNFPDFLNP KTVSWWGDEI RRFHELVPVD GLWIDMNEAS 480 NFCSGLCKIP KGKQCPTGTG PGWVCCLDCK NITKTRWDDP PYKINASGLQ VPIGFKTIAT 540 SAYHYNGVLE YDAHSIYGFS QSIATHKALL GLEGKRPFIL SRSTFVGSGH YAAHWTGDNK 600 GTWEDLKYSI STMLNFGIFG VPMVGSDICG FYPAPTEELC NRWIEVGAFY PFSRDHANYY 660 SPRQELYQWE SVAESARNAL GMRYKLLPFL YTLNYEAHLS GAPIARPLFF SFPNYVECYN 720 VSTQFLLGSS LMVSPVLEQG KSQVKALFPP GSWYNVFDMT QAISSKDGKF VTLDAPLHVV 780 NVHLYQNTIL PMQQGGLISK EARMTPFSLV VTFPAGASGV QAKGKLYLDE DELPEMKLGN 840 GYSTYVDFFA TTGNGTVKIW SEVQEGKFAL SKGWIIDSVT VLGLGGSGKA STLEINGSPT 900 NANSKIEFNA SEQST* 960 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
pfam01055 | Glyco_hydro_31 | 3.0e-87 | 299 | 485 | 187 | + Glycosyl hydrolases family 31. Glycosyl hydrolases are key enzymes of carbohydrate metabolism. Family 31 comprises of enzymes that are, or similar to, alpha- galactosidases. | ||
cd06602 | GH31_MGAM_SI_GAA | 6.0e-89 | 318 | 482 | 169 | + This family includes the following three closely related glycosyl hydrolase family 31 (GH31) enzymes: maltase-glucoamylase (MGAM), sucrase-isomaltase (SI), and lysosomal acid alpha-glucosidase (GAA), also known as acid-maltase. MGAM is one of the two enzymes responsible for catalyzing the last glucose-releasing step in starch digestion. SI is implicated in the digestion of dietary starch and major disaccharides such as sucrose and isomaltose, while GAA degrades glycogen in the lysosome, cleaving both alpha-1,4 and alpha-1,6 glucosidic linkages. MGAM and SI are anchored to small-intestinal brush-border epithelial cells. The absence of SI from the brush border membrane or its malfunction is associated with malabsorption disorders such as congenital sucrase-isomaltase deficiency (CSID). The domain architectures of MGAM and SI include two tandem GH31 catalytic domains, an N-terminal domain found near the membrane-bound end, and a C-terminal luminal domain. Both of the tandem GH31 domains of MGAM and SI are included in this family. The domain architecture of GAA includes an N-terminal TFF (trefoil factor family) domain in addition to the GH31 catalytic domain. Deficient GAA expression causes pompe disease, an autosomal recessive genetic disorder also known as glycogen storage disease type II (GSDII). | ||
cd06602 | GH31_MGAM_SI_GAA | 4.0e-93 | 551 | 719 | 170 | + This family includes the following three closely related glycosyl hydrolase family 31 (GH31) enzymes: maltase-glucoamylase (MGAM), sucrase-isomaltase (SI), and lysosomal acid alpha-glucosidase (GAA), also known as acid-maltase. MGAM is one of the two enzymes responsible for catalyzing the last glucose-releasing step in starch digestion. SI is implicated in the digestion of dietary starch and major disaccharides such as sucrose and isomaltose, while GAA degrades glycogen in the lysosome, cleaving both alpha-1,4 and alpha-1,6 glucosidic linkages. MGAM and SI are anchored to small-intestinal brush-border epithelial cells. The absence of SI from the brush border membrane or its malfunction is associated with malabsorption disorders such as congenital sucrase-isomaltase deficiency (CSID). The domain architectures of MGAM and SI include two tandem GH31 catalytic domains, an N-terminal domain found near the membrane-bound end, and a C-terminal luminal domain. Both of the tandem GH31 domains of MGAM and SI are included in this family. The domain architecture of GAA includes an N-terminal TFF (trefoil factor family) domain in addition to the GH31 catalytic domain. Deficient GAA expression causes pompe disease, an autosomal recessive genetic disorder also known as glycogen storage disease type II (GSDII). | ||
pfam01055 | Glyco_hydro_31 | 5.0e-109 | 544 | 790 | 251 | + Glycosyl hydrolases family 31. Glycosyl hydrolases are key enzymes of carbohydrate metabolism. Family 31 comprises of enzymes that are, or similar to, alpha- galactosidases. | ||
cd06604 | GH31_glucosidase_II_MalA | 3.0e-122 | 318 | 701 | 390 | + Alpha-glucosidase II (alpha-D-glucoside glucohydrolase) is a glycosyl hydrolase family 31 (GH31) enzyme, found in bacteria and plants, which has exo-alpha-1,4-glucosidase and oligo-1,6-glucosidase activities. Alpha-glucosidase II has been characterized in Bacillus thermoamyloliquefaciens where it forms a homohexamer. This family also includes the MalA alpha-glucosidase from Sulfolobus sulfataricus and the AglA alpha-glucosidase from Picrophilus torridus. MalA is part of the carbohydrate-metabolizing machinery that allows this organism to utilize carbohydrates, such as maltose, as the sole carbon and energy source. |
Gene Ontology | |
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GO Term | Description |
GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
GO:0005975 | carbohydrate metabolic process |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
EMBL | CAA10382.2 | 0 | 49 | 913 | 30 | 896 | alpha-D-xylosidase [Tropaeolum majus] |
RefSeq | XP_002282429.1 | 0 | 58 | 913 | 27 | 882 | PREDICTED: hypothetical protein isoform 1 [Vitis vinifera] |
RefSeq | XP_002311455.1 | 0 | 60 | 913 | 15 | 869 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002315944.1 | 0 | 37 | 913 | 5 | 887 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002531635.1 | 0 | 60 | 913 | 35 | 889 | alpha-glucosidase, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3w38_A | 0 | 37 | 884 | 18 | 851 | A Chain A, Crystal Structure Of Galactose Oxidase Mutant W290f |
PDB | 3w37_A | 0 | 37 | 884 | 18 | 851 | A Chain A, Crystal Structure Of Galactose Oxidase Mutant W290f |
PDB | 3lpp_D | 0 | 63 | 913 | 80 | 879 | A Chain A, Crystal Structure Of Galactose Oxidase Mutant W290f |
PDB | 3lpp_C | 0 | 63 | 913 | 80 | 879 | A Chain A, Crystal Structure Of Galactose Oxidase Mutant W290f |
PDB | 3lpp_B | 0 | 63 | 913 | 80 | 879 | A Chain A, Crystal Structure Of Galactose Oxidase Mutant W290f |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
HO795307 | 633 | 281 | 913 | 0 |
HO777477 | 530 | 51 | 579 | 0 |
CX043828 | 296 | 202 | 497 | 0 |
DY293314 | 298 | 60 | 357 | 0 |
HO777477 | 44 | 567 | 610 | 1.5 |
Sequence Alignments (This image is cropped. Click for full image.) |
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