| Basic Information | |
|---|---|
| Species | Chlamydomonas reinhardtii |
| Cazyme ID | Cre02.g091750.t3.1 |
| Family | CBM20 |
| Protein Properties | Length: 794 Molecular Weight: 81349 Isoelectric Point: 10.3897 |
| Chromosome | Chromosome/Scaffold: 2 Start: 2449363 End: 2453944 |
| Description | catalytics;carbohydrate kinases;phosphoglucan, water dikinases |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| CBM20 | 59 | 146 | 4.5e-24 |
| LNFKVGYRVNFGQTIAMIGSGDALGNWDPKRSIAMKWTDGDYWTVELNVAPGRSALDLEYKYVVVNSDGHIGYWKPGSNYKVTLPLNS | |||
| Full Sequence |
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| Protein Sequence Length: 794 Download |
| MVNQLSSRPG VHAGAPAAMG ACRGPGLLLR RSLTAVRCFK SNSSDDAGAR SSTVSSLKLN 60 FKVGYRVNFG QTIAMIGSGD ALGNWDPKRS IAMKWTDGDY WTVELNVAPG RSALDLEYKY 120 VVVNSDGHIG YWKPGSNYKV TLPLNSSGSK VPKRVKVADA WDDSYKKVDV EEAEALPASP 180 SSKGTSAAAA APPPPPKAAP PPPAAAAASS SAPASGAAQP KAAAQAPPAA APPPPPPQQG 240 KQQQQQPAAA AQQAAPSNGA GPSQKPQNGI PRATSPRSGS APAGFAAITS IPAIHPTLMA 300 EQERLEDGVK MSLEDLQNQM DKHQQLRERT DEAKLAAAEA RASVLAEAVA ALTKEVASLK 360 DQLSDMRALL TSTSELLVSV LRQPLPGSGS GGATQQVFVG APGFAAFAMQ QPQQQHTTGG 420 GMASWWGPQQ LPQAGQGQYS QYSPAALQHS EQPAAASWAP AAAAPTVIPV GTEQPAVDVA 480 PVPAAQSVGG AGLVLPQLPA VAARRVDARR TSLVRTMVRR HAAGHPRVAV LGRGLAARKA 540 AAKAAAPDPA PAAHPESPTD VTPQAPSAPS SQPVMVLTPA AASPHPSSNS RDTGRQQADS 600 SAPIAPAAPA NRARAASAAA LSAPPVRAPM ASAAKAAAVA AVLLPVVCGV GRGAKRGARG 660 RFNMQQYRDA VRARAKDAPA CIELSAVKNE GVWADVNQPQ QLTPTEFMQE AGLKGKNWKA 720 SIRVVANGEV GAALSEVLAQ VADHPAAVEV LVADRIVAAA NNRVVAYNKA LKAVQEYNRL 780 PPSAGPASQE KAQ* 840 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd05818 | CBM20_water_dikinase | 2.0e-12 | 57 | 143 | 87 | + Phosphoglucan water dikinase (also known as alpha-glucan water dikinase), N-terminal CBM20 (carbohydrate-binding module, family 20) domain. This domain is found in the chloroplast-encoded phosphoglucan water dikinase, one of two enzymes involved in the phosphorylation of plant starches. In addition to the CBM20 domain, phosphoglucan water dikinase contains a C-terminal pyruvate binding domain. The CBM20 domain is found in a large number of starch degrading enzymes including alpha-amylase, beta-amylase, glucoamylase, and CGTase (cyclodextrin glucanotransferase). CBM20 is also present in proteins that have a regulatory role in starch metabolism in plants (e.g. alpha-amylase) or glycogen metabolism in mammals (e.g. laforin). CBM20 folds as an antiparallel beta-barrel structure with two starch binding sites. These two sites are thought to differ functionally with site 1 acting as the initial starch recognition site and site 2 involved in the specific recognition of appropriate regions of starch. | ||
| cd02861 | E_set_pullulanase_like | 3.0e-15 | 59 | 141 | 84 | + Early set domain associated with the catalytic domain of pullulanase-like proteins. E or "early" set domains are associated with the catalytic domain of pullulanase at either the N-terminal or C-terminal end, and in a few instances at both ends. Pullulanase (also called dextrinase or alpha-dextrin endo-1,6-alpha glucosidase) is an enzyme with action similar to that of isoamylase; it cleaves 1,6-alpha-glucosidic linkages in pullulan, amylopectin, and glycogen, and in alpha-and beta-amylase limit-dextrins of amylopectin and glycogen. The E set domain of pullulanase may be related to the immunoglobulin and/or fibronectin type III superfamilies. These domains are associated with different types of catalytic domains at either the N-terminal or C-terminal end and may be involved in homodimeric/tetrameric/dodecameric interactions. Members of this family include members of the alpha amylase family, sialidase, galactose oxidase, cellulase, cellulose, hyaluronate lyase, chitobiase, and chitinase. This domain is also a member of the CBM48 (Carbohydrate Binding Module 48) family whose members include maltooligosyl trehalose synthase, starch branching enzyme, glycogen branching enzyme, glycogen debranching enzyme, isoamylase, and the beta subunit of AMP-activated protein kinase. | ||
| pfam00686 | CBM_20 | 2.0e-16 | 61 | 149 | 92 | + Starch binding domain. | ||
| smart01065 | CBM_2 | 7.0e-17 | 61 | 143 | 86 | + Starch binding domain. | ||
| cd05467 | CBM20 | 8.0e-19 | 58 | 149 | 95 | + The family 20 carbohydrate-binding module (CBM20), also known as the starch-binding domain, is found in a large number of starch degrading enzymes including alpha-amylase, beta-amylase, glucoamylase, and CGTase (cyclodextrin glucanotransferase). CBM20 is also present in proteins that have a regulatory role in starch metabolism in plants (e.g. alpha-amylase) or glycogen metabolism in mammals (e.g. laforin). CBM20 folds as an antiparallel beta-barrel structure with two starch binding sites. These two sites are thought to differ functionally with site 1 acting as the initial starch recognition site and site 2 involved in the specific recognition of appropriate regions of starch. | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CAB42581.1 | 0.00000000000005 | 58 | 179 | 23 | 132 | putative degreening-enhanced protein [Auxenochlorella protothecoides] |
| RefSeq | XP_001696825.1 | 0.000000000002 | 56 | 162 | 23 | 122 | hypothetical protein CHLREDRAFT_150073 [Chlamydomonas reinhardtii] |
| RefSeq | XP_001701978.1 | 0 | 56 | 161 | 1 | 106 | predicted protein [Chlamydomonas reinhardtii] |
| RefSeq | XP_627384.1 | 4e-16 | 45 | 161 | 41 | 152 | starch binding domain containing protein, possible plant origin [Cryptosporidium parvum Iowa II] |
| RefSeq | XP_665094.1 | 6e-16 | 45 | 161 | 41 | 152 | glucan 1,4-alpha-glucosidase precursor [Cryptosporidium hominis TU502] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1cyg_A | 0.00002 | 68 | 151 | 592 | 675 | A Chain A, Cyclodextrin Glucanotransferase (E.C.2.4.1.19) (Cgtase) |
| PDB | 1kum_A | 0.0002 | 52 | 161 | 2 | 107 | A Chain A, Cyclodextrin Glucanotransferase (E.C.2.4.1.19) (Cgtase) |
| PDB | 1kul_A | 0.0002 | 52 | 161 | 2 | 107 | A Chain A, Cyclodextrin Glucanotransferase (E.C.2.4.1.19) (Cgtase) |
| PDB | 1acz_A | 0.0002 | 52 | 161 | 2 | 107 | A Chain A, Glucoamylase, Granular Starch-Binding Domain Complex With Cyclodextrin, Nmr, 5 Structures |
| PDB | 1ac0_A | 0.0002 | 52 | 161 | 2 | 107 | A Chain A, Glucoamylase, Granular Starch-Binding Domain Complex With Cyclodextrin, Nmr, Minimized Average Structure |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| BI721586 | 157 | 1 | 157 | 0 |
| BI999455 | 146 | 1 | 146 | 0 |
| BQ809401 | 122 | 1 | 122 | 0 |
| BI724636 | 123 | 19 | 141 | 0 |
| FD811317 | 153 | 22 | 174 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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