| Basic Information | |
|---|---|
| Species | Chlamydomonas reinhardtii |
| Cazyme ID | Cre02.g112400.t1.3 |
| Family | GH18 |
| Protein Properties | Length: 396 Molecular Weight: 43656.6 Isoelectric Point: 6.3647 |
| Chromosome | Chromosome/Scaffold: 2 Start: 6018860 End: 6023095 |
| Description | |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH18 | 74 | 354 | 6.40393e-43 |
| EVMCAAHAAGVRAIMNAGDVSAAIADDKARELWVDAQVELMRRHWYDGINFDYELPAKMGSQLSTNYTRLVEATSRAFKRVNPHAEISVDVPWAAFGNDG RDYDWKGLAKVSDRLFVMSYDQQAQVFGRCVAAANSPLPQVAKGVQQYLDLGIPPSKLILGVAWYGYDYPCQPDGRGVKPGPDTDLCVLQPVEYRGAPCS DAAGVQRNFWEFMAELRAGRNSTALTWDPLASSPRFNYVDKTKGQVHQVWFETPTSLALRYRLARDLGLKGVGTWNLGAVE | |||
| Full Sequence |
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| Protein Sequence Length: 396 Download |
| MGQLSVVWCL TSGVTETVKE PASCPCSSPA LCSFVRTRRE REFMGFFYPE DMPVDLLDWS 60 QISSLAWVPA QRPEVMCAAH AAGVRAIMNA GDVSAAIADD KARELWVDAQ VELMRRHWYD 120 GINFDYELPA KMGSQLSTNY TRLVEATSRA FKRVNPHAEI SVDVPWAAFG NDGRDYDWKG 180 LAKVSDRLFV MSYDQQAQVF GRCVAAANSP LPQVAKGVQQ YLDLGIPPSK LILGVAWYGY 240 DYPCQPDGRG VKPGPDTDLC VLQPVEYRGA PCSDAAGVQR NFWEFMAELR AGRNSTALTW 300 DPLASSPRFN YVDKTKGQVH QVWFETPTSL ALRYRLARDL GLKGVGTWNL GAVEHSGTAG 360 LEARADTAAM WEAVRVFTGV RMDTTTMKGM RLTGG* 420 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| COG3858 | COG3858 | 1.0e-23 | 87 | 352 | 288 | + Predicted glycosyl hydrolase [General function prediction only] | ||
| pfam00704 | Glyco_hydro_18 | 1.0e-29 | 94 | 352 | 265 | + Glycosyl hydrolases family 18. | ||
| smart00636 | Glyco_18 | 3.0e-33 | 94 | 352 | 272 | + Glyco_18 domain. | ||
| cd02874 | GH18_CFLE_spore_hydrolase | 2.0e-34 | 78 | 351 | 290 | + Cortical fragment-lytic enzyme (CFLE) is a peptidoglycan hydrolase involved in bacterial endospore germination. CFLE is expressed as an inactive preprotein (called SleB) in the forespore compartment of sporulating cells. SleB translocates across the forespore inner membrane and is deposited as a mature enzyme in the cortex layer of the spore. As part of a sensory mechanism capable of initiating germination, CFLE degrades a spore-specific peptidoglycan constituent called muramic-acid delta-lactam that comprises the outer cortex. CFLE has a C-terminal glycosyl hydrolase family 18 (GH18) catalytic domain as well as two N-terminal LysM peptidoglycan-binding domains. In addition to SleB, this family includes YaaH, YdhD, and YvbX from Bacillus subtilis. | ||
| cd02875 | GH18_chitobiase | 8.0e-102 | 42 | 373 | 340 | + Chitobiase (also known as di-N-acetylchitobiase) is a lysosomal glycosidase that hydrolyzes the reducing-end N-acetylglucosamine from the chitobiose core of oligosaccharides during the ordered degradation of asparagine-linked glycoproteins in eukaryotes. Chitobiase can only do so if the asparagine that joins the oligosaccharide to protein is previously removed by a glycosylasparaginase. Chitobiase is therefore the final step in the lysosomal degradation of the protein/carbohydrate linkage component of asparagine-linked glycoproteins. The catalytic domain of chitobiase is an eight-stranded alpha/beta barrel fold similar to that of other family 18 glycosyl hydrolases such as hevamine and chitotriosidase. | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| RefSeq | NP_001011500.1 | 0 | 22 | 375 | 25 | 364 | di-N-acetylchitobiase [Xenopus (Silurana) tropicalis] |
| RefSeq | NP_001079731.1 | 0 | 22 | 375 | 26 | 366 | di-N-acetylchitobiase [Xenopus laevis] |
| RefSeq | XP_001367069.1 | 0 | 24 | 376 | 123 | 462 | PREDICTED: similar to di-N-acetylchitobiase [Monodelphis domestica] |
| RefSeq | XP_001699683.1 | 0 | 88 | 376 | 1 | 289 | predicted protein [Chlamydomonas reinhardtii] |
| RefSeq | XP_001742289.1 | 0 | 26 | 377 | 26 | 369 | hypothetical protein [Monosiga brevicollis MX1] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3cz8_B | 0.0000000000004 | 93 | 354 | 86 | 312 | A Chain A, Crystal Structure Of Putative Sporulation-Specific Glycosylase Ydhd From Bacillus Subtilis |
| PDB | 3cz8_A | 0.0000000000004 | 93 | 354 | 86 | 312 | A Chain A, Crystal Structure Of Putative Sporulation-Specific Glycosylase Ydhd From Bacillus Subtilis |
| PDB | 3rme_B | 0.00000002 | 94 | 242 | 86 | 250 | A Chain A, Crystal Structure Of Putative Sporulation-Specific Glycosylase Ydhd From Bacillus Subtilis |
| PDB | 3rme_A | 0.00000002 | 94 | 242 | 86 | 250 | A Chain A, Crystal Structure Of Putative Sporulation-Specific Glycosylase Ydhd From Bacillus Subtilis |
| PDB | 3rm9_B | 0.00000002 | 94 | 242 | 86 | 250 | A Chain A, Crystal Structure Of Putative Sporulation-Specific Glycosylase Ydhd From Bacillus Subtilis |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| BI719178 | 117 | 83 | 199 | 0 |
| BI719178 | 83 | 1 | 83 | 0 |
| DV150160 | 222 | 97 | 311 | 1e-34 |
| FG053745 | 203 | 34 | 231 | 4e-28 |
| FG053746 | 189 | 198 | 383 | 3e-27 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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