| Basic Information | |
|---|---|
| Species | Chlamydomonas reinhardtii |
| Cazyme ID | Cre08.g384750.t1.1 |
| Family | GH13 |
| Protein Properties | Length: 502 Molecular Weight: 55596.2 Isoelectric Point: 6.4111 |
| Chromosome | Chromosome/Scaffold: 8 Start: 4859949 End: 4865606 |
| Description | alpha-amylase-like 3 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH13 | 109 | 413 | 3.00004e-41 |
| GNWYGRVQAAIPELAALQVSHVWLPPPSRSVSKEGYLPGQLYDLDSEYGTKEQLTQLCAALKAAGISPMADIVINHRCADEQVDGVYNNFLDEIDHAGKS IDWGRWAITADDPHFNGEGNPDTGDDFGAAPDLDHANPELRAALVDWLKHLKTEVGFTGWRFDYARGYAARFIAEYVDKTVGKDVLNVGEYWTEAQWQGE HLDFNQDGARQVLCNWLDQSNGRSAAFDFPTKSLLQEACRHTQYNRLRDERGKAPGLVGWWPAKAVTFIENHDTGSTQQHWPFPSEYVGTGYAYLLTHPG IPTIF | |||
| Full Sequence |
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| Protein Sequence Length: 502 Download |
| MALGMHAQQQ QHRLQARTAN RRTAAAPLRP AQPANAAASR SRAQRGLRLR VMAQAPPFTG 60 GTDTKPDGAV ATATHSAAPA AAARSTRSPY GKAILLQGFA WDSWQKGGGN WYGRVQAAIP 120 ELAALQVSHV WLPPPSRSVS KEGYLPGQLY DLDSEYGTKE QLTQLCAALK AAGISPMADI 180 VINHRCADEQ VDGVYNNFLD EIDHAGKSID WGRWAITADD PHFNGEGNPD TGDDFGAAPD 240 LDHANPELRA ALVDWLKHLK TEVGFTGWRF DYARGYAARF IAEYVDKTVG KDVLNVGEYW 300 TEAQWQGEHL DFNQDGARQV LCNWLDQSNG RSAAFDFPTK SLLQEACRHT QYNRLRDERG 360 KAPGLVGWWP AKAVTFIENH DTGSTQQHWP FPSEYVGTGY AYLLTHPGIP TIFWDHVFEW 420 GEPLRQEITA LAQLRQRVGL HSESKLEILA AEPDMYVARV AEKVTVKLGP RCDMGDLLPK 480 EDDGWKFVMS GKDWAVWEKT N* 540 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PRK09441 | PRK09441 | 4.0e-50 | 94 | 437 | 413 | + cytoplasmic alpha-amylase; Reviewed | ||
| PLN02784 | PLN02784 | 6.0e-130 | 91 | 500 | 412 | + alpha-amylase | ||
| PLN00196 | PLN00196 | 6.0e-138 | 94 | 499 | 412 | + alpha-amylase; Provisional | ||
| PLN02361 | PLN02361 | 6.0e-138 | 91 | 499 | 412 | + alpha-amylase | ||
| cd11314 | AmyAc_arch_bac_plant_AmyA | 2.0e-148 | 94 | 446 | 355 | + Alpha amylase catalytic domain found in archaeal, bacterial, and plant Alpha-amylases (also called 1,4-alpha-D-glucan-4-glucanohydrolase). AmyA (EC 3.2.1.1) catalyzes the hydrolysis of alpha-(1,4) glycosidic linkages of glycogen, starch, related polysaccharides, and some oligosaccharides. This group includes AmyA from bacteria, archaea, water fleas, and plants. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAA98615.1 | 0 | 94 | 500 | 26 | 427 | alpha-amylase [Hordeum vulgare subsp. vulgare] |
| RefSeq | XP_001694202.1 | 0 | 96 | 501 | 5 | 408 | alpha-amylase [Chlamydomonas reinhardtii] |
| RefSeq | XP_001694304.1 | 0 | 1 | 501 | 42 | 404 | alpha-amylase-like protein [Chlamydomonas reinhardtii] |
| RefSeq | XP_002500155.1 | 0 | 30 | 499 | 52 | 561 | glycoside hydrolase family 13 protein [Micromonas sp. RCC299] |
| RefSeq | XP_002506073.1 | 0 | 119 | 500 | 2 | 385 | glycoside hydrolase family 13 protein [Micromonas sp. RCC299] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1bg9_A | 0 | 94 | 500 | 2 | 403 | A Chain A, Sugar Tongs Mutant S378p In Complex With Acarbose |
| PDB | 1ava_B | 0 | 94 | 500 | 2 | 403 | A Chain A, Amy2BASI PROTEIN-Protein Complex From Barley Seed |
| PDB | 1ava_A | 0 | 94 | 500 | 2 | 403 | A Chain A, Amy2BASI PROTEIN-Protein Complex From Barley Seed |
| PDB | 1amy_A | 0 | 94 | 500 | 2 | 403 | A Chain A, Amy2BASI PROTEIN-Protein Complex From Barley Seed |
| PDB | 2qpu_C | 0 | 94 | 500 | 3 | 405 | A Chain A, Sugar Tongs Mutant S378p In Complex With Acarbose |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| starch degradation I | RXN-1823 | EC-3.2.1.1 | α-amylase |
| starch degradation I | RXN-1825 | EC-3.2.1.1 | α-amylase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| BI716207 | 201 | 301 | 501 | 0 |
| HO420508 | 412 | 90 | 499 | 0 |
| HO409542 | 414 | 92 | 502 | 0 |
| HO476908 | 411 | 93 | 501 | 0 |
| HO780661 | 410 | 91 | 499 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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