| Basic Information | |
|---|---|
| Species | Chlamydomonas reinhardtii |
| Cazyme ID | Cre09.g415600.t1.3 |
| Family | CBM20 |
| Protein Properties | Length: 444 Molecular Weight: 47350 Isoelectric Point: 5.8609 |
| Chromosome | Chromosome/Scaffold: 9 Start: 7689542 End: 7693657 |
| Description | Carbohydrate-binding-like fold |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| CBM20 | 117 | 202 | 6.2e-28 |
| VQVRFCLKYRTSYGQSVKIIGSHAKLGNWDINKALVLSWTDGDRWVATIELPAGSVYEYKYVLVDHDGRSALAWQGGSNSVLAIGD | |||
| Full Sequence |
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| Protein Sequence Length: 444 Download |
| MQRTSQAFAS GSNLQKRTTR DATVAQGVRG KAQGLSTQVV SNPRSLAAGA SLVPRRSTAN 60 WVAEVSTSAQ RLLEAPASYR QGGAQANAGP NALMATDGKA SSLRKLVVDG VDDSPNVQVR 120 FCLKYRTSYG QSVKIIGSHA KLGNWDINKA LVLSWTDGDR WVATIELPAG SVYEYKYVLV 180 DHDGRSALAW QGGSNSVLAI GDQDEQGVEV QDNWHNNPGQ AAVVAGGQTL TRETKLQAWA 240 EEMKQYRALA RSAQFELSRK AEELQASKAQ VARLKMELSM SLKAREELES RLADLEGENL 300 SLRAQVAQSQ IAMKSTLEEA IKLLQQEIEE GEEELYSTGL GDEELEAAAS GSGSSGASSS 360 SSNGSGWGWG RSKEAETVYA GSNGSSSNGS GGSNGATFER FGFRLSGNTF DQPQQTGSEF 420 GHNSGSRSNN NVLADWRTRL GSQ* 480 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd02859 | E_set_AMPKbeta_like_N | 4.0e-17 | 119 | 214 | 101 | + N-terminal Early set domain, a glycogen binding domain, associated with the catalytic domain of AMP-activated protein kinase beta subunit. E or "early" set domains are associated with the catalytic domain of AMP-activated protein kinase beta subunit glycogen binding domain at the N-terminal end. AMPK is a metabolic stress sensing protein that senses AMP/ATP and has recently been found to act as a glycogen sensor as well. The protein functions as an alpha-beta-gamma heterotrimer. This N-terminal domain is the glycogen binding domain of the beta subunit. This domain is also a member of the CBM48 (Carbohydrate Binding Module 48) family whose members include pullulanase, maltooligosyl trehalose synthase, starch branching enzyme, glycogen branching enzyme, glycogen debranching enzyme, and isoamylase. | ||
| cd02856 | E_set_GDE_Isoamylase_N | 2.0e-18 | 113 | 214 | 106 | + N-terminal Early set domain associated with the catalytic domain of Glycogen debranching enzyme and bacterial isoamylase (also called glycogen 6-glucanohydrolase). E or "early" set domains are associated with the catalytic domain of the glycogen debranching enzyme at the N-terminal end. Glycogen debranching enzymes have both 4-alpha-glucanotransferase and amylo-1,6-glucosidase activities. As a transferase, it transfers a segment of the 1,4-alpha-D-glucan to a new 4-position in an acceptor, which may be glucose or another 1,4-alpha-D-glucan. As a glucosidase, it catalyzes the endohydrolysis of 1,6-alpha-D-glucoside linkages at points of branching in chains of 1,4-linked alpha-D-glucose residues. Bacterial isoamylases are also included in this subfamily. Isoamylase is one of the starch-debranching enzymes that catalyze the hydrolysis of alpha-1,6-glucosidic linkages specific in alpha-glucans such as amylopectin or glycogen. Isoamylase contains a bound calcium ion, but this is not in the same position as the conserved calcium ion that has been reported in other alpha-amylase family enzymes. The N-terminal domain of glycogen debranching enzyme may be related to the immunoglobulin and/or fibronectin type III superfamilies. These domains are associated with different types of catalytic domains at either the N-terminal or C-terminal end and may be involved in homodimeric/tetrameric/dodecameric interactions. Members of this family include members of the alpha amylase family, sialidase, galactose oxidase, cellulase, cellulose, hyaluronate lyase, chitobiase, and chitinase, among others. | ||
| smart01065 | CBM_2 | 1.0e-18 | 117 | 202 | 90 | + Starch binding domain. | ||
| pfam00686 | CBM_20 | 4.0e-22 | 117 | 201 | 88 | + Starch binding domain. | ||
| cd05467 | CBM20 | 3.0e-30 | 118 | 214 | 100 | + The family 20 carbohydrate-binding module (CBM20), also known as the starch-binding domain, is found in a large number of starch degrading enzymes including alpha-amylase, beta-amylase, glucoamylase, and CGTase (cyclodextrin glucanotransferase). CBM20 is also present in proteins that have a regulatory role in starch metabolism in plants (e.g. alpha-amylase) or glycogen metabolism in mammals (e.g. laforin). CBM20 folds as an antiparallel beta-barrel structure with two starch binding sites. These two sites are thought to differ functionally with site 1 acting as the initial starch recognition site and site 2 involved in the specific recognition of appropriate regions of starch. | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CAL53465.1 | 2e-18 | 111 | 223 | 74 | 188 | glucan 1,4-alpha-glucosidase (ISS) [Ostreococcus tauri] |
| EMBL | CAL56006.1 | 2e-18 | 80 | 200 | 18 | 127 | unnamed protein product [Ostreococcus tauri] |
| RefSeq | XP_001418210.1 | 3e-19 | 117 | 236 | 13 | 135 | predicted protein [Ostreococcus lucimarinus CCE9901] |
| RefSeq | XP_001696825.1 | 0 | 94 | 443 | 1 | 323 | hypothetical protein CHLREDRAFT_150073 [Chlamydomonas reinhardtii] |
| RefSeq | XP_002508103.1 | 2e-20 | 117 | 294 | 291 | 483 | carbohydrate-binding module family 20 protein [Micromonas sp. RCC299] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1kum_A | 0.00000006 | 117 | 195 | 7 | 87 | A Chain A, Glucoamylase, Granular Starch-Binding Domain Complex With Cyclodextrin, Nmr, Minimized Average Structure |
| PDB | 1kul_A | 0.00000006 | 117 | 195 | 7 | 87 | A Chain A, Glucoamylase, Granular Starch-Binding Domain Complex With Cyclodextrin, Nmr, Minimized Average Structure |
| PDB | 1acz_A | 0.00000006 | 117 | 195 | 7 | 87 | A Chain A, Glucoamylase, Granular Starch-Binding Domain Complex With Cyclodextrin, Nmr, 5 Structures |
| PDB | 1ac0_A | 0.00000006 | 117 | 195 | 7 | 87 | A Chain A, Glucoamylase, Granular Starch-Binding Domain Complex With Cyclodextrin, Nmr, Minimized Average Structure |
| PDB | 2vn7_A | 0.0000003 | 117 | 200 | 497 | 582 | A Chain A, Glucoamylase, Granular Starch-Binding Domain Complex With Cyclodextrin, Nmr, Minimized Average Structure |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| BP094955 | 95 | 9 | 103 | 1.99965e-42 |
| FD844544 | 64 | 93 | 156 | 2e-26 |
| DC994677 | 95 | 119 | 213 | 6e-23 |
| FD844543 | 53 | 104 | 156 | 7e-21 |
| GR823708 | 95 | 106 | 200 | 2e-17 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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