| Basic Information | |
|---|---|
| Species | Chlamydomonas reinhardtii |
| Cazyme ID | Cre12.g514200.t1.2 |
| Family | AA3 |
| Protein Properties | Length: 612 Molecular Weight: 64459.5 Isoelectric Point: 9.4814 |
| Chromosome | Chromosome/Scaffold: 12 Start: 3638157 End: 3644852 |
| Description | Glucose-methanol-choline (GMC) oxidoreductase family protein |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| AA3 | 43 | 601 | 0 |
| SEKFDYVLVGGGTASCVLANKLSADGNKKVLVLEAGPTGDAMEVAVPAGITRLFAHPVMDWGMSSLTQKQLVAREIYLARGRMLGGSSGSNATLYHRGSA ADYDAWGLEGWSSKDVLDWFVKAECYADGPKPYHGTGGSMNTEQPRYENVLHDEFFKAAAATGLPANPDFNDWSHPQDGFGEFQVSQKKGQRADTYRTYL KPAMARGNLKVVIGARATKVNIEKGSSGARTTGVEYAMQQFGDRFTAELAPGGEVLMCSGAVHTPHLLMLSGVGPAATLKEHGIDVVSDLSGVGQNLQDH PAAVLAARAKPEFEKLSVTSEVYDDKCNIKLGAVAQYLFQRRGPLATTGCDHGAFVRTSSSLSQPDLQMRFVPGCALDPDGVKSYIVFGELKKQGRAWPG GITLQLLAIRAKSKGSIGLKAADPFINPAININYFSDPADLATLVNAVKMARKIAAQEPLKKYLQEETFPGERASSDKDLEEYIRRTVHSGNALVGTAAM GASPAAGAVVSSADLKVFGVEGLRVVDASVLPRIPGGQTGAATVMVAERAAALLRGQAT | |||
| Full Sequence |
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| Protein Sequence Length: 612 Download |
| MMLGPKTVTR GATKGAAPRS MAARRVGGAR RLSVRAAAGP AGSEKFDYVL VGGGTASCVL 60 ANKLSADGNK KVLVLEAGPT GDAMEVAVPA GITRLFAHPV MDWGMSSLTQ KQLVAREIYL 120 ARGRMLGGSS GSNATLYHRG SAADYDAWGL EGWSSKDVLD WFVKAECYAD GPKPYHGTGG 180 SMNTEQPRYE NVLHDEFFKA AAATGLPANP DFNDWSHPQD GFGEFQVSQK KGQRADTYRT 240 YLKPAMARGN LKVVIGARAT KVNIEKGSSG ARTTGVEYAM QQFGDRFTAE LAPGGEVLMC 300 SGAVHTPHLL MLSGVGPAAT LKEHGIDVVS DLSGVGQNLQ DHPAAVLAAR AKPEFEKLSV 360 TSEVYDDKCN IKLGAVAQYL FQRRGPLATT GCDHGAFVRT SSSLSQPDLQ MRFVPGCALD 420 PDGVKSYIVF GELKKQGRAW PGGITLQLLA IRAKSKGSIG LKAADPFINP AININYFSDP 480 ADLATLVNAV KMARKIAAQE PLKKYLQEET FPGERASSDK DLEEYIRRTV HSGNALVGTA 540 AMGASPAAGA VVSSADLKVF GVEGLRVVDA SVLPRIPGGQ TGAATVMVAE RAAALLRGQA 600 TIAPSRQPVA V* 660 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| pfam00732 | GMC_oxred_N | 8.0e-38 | 116 | 344 | 236 | + GMC oxidoreductase. This family of proteins bind FAD as a cofactor. | ||
| TIGR03970 | Rv0697 | 7.0e-56 | 47 | 577 | 543 | + dehydrogenase, Rv0697 family. This model describes a set of dehydrogenases belonging to the glucose-methanol-choline oxidoreductase (GMC oxidoreductase) family. Members of the present family are restricted to Actinobacterial genome contexts containing also members of families TIGR03962 and TIGR03969 (the mycofactocin system), and are proposed to be uniform in function. | ||
| TIGR01810 | betA | 2.0e-97 | 47 | 599 | 560 | + choline dehydrogenase. Choline dehydrogenase catalyzes the conversion of exogenously supplied choline into the intermediate glycine betaine aldehyde, as part of a two-step oxidative reaction leading to the formation of osmoprotectant betaine. This enzymatic system can be found in both gram-positive and gram-negative bacteria. As in Escherichia coli , Staphylococcus xylosus , and Sinorhizobium meliloti, this enzyme is found associated in a transciptionally co-induced gene cluster with betaine aldehyde dehydrogenase, the second catalytic enzyme in this reaction. Other gram-positive organisms have been shown to employ a different enzymatic system, utlizing a soluable choline oxidase or type III alcohol dehydrogenase instead of choline dehydrogenase. This enzyme is a member of the GMC oxidoreductase family (pfam00732 and pfam05199), sharing a common evoluntionary origin and enzymatic reaction with alcohol dehydrogenase. Outgrouping from this model, Caulobacter crescentus shares sequence homology with choline dehydrogenase, yet other genes participating in this enzymatic reaction have not currently been identified [Cellular processes, Adaptations to atypical conditions]. | ||
| COG2303 | BetA | 2.0e-105 | 43 | 581 | 548 | + Choline dehydrogenase and related flavoproteins [Amino acid transport and metabolism] | ||
| PRK02106 | PRK02106 | 4.0e-134 | 43 | 611 | 581 | + choline dehydrogenase; Validated | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ABY65992.1 | 0 | 46 | 605 | 2 | 523 | alcohol dehydrogenase [Actinomadura madurae] |
| RefSeq | XP_001703004.1 | 0 | 1 | 611 | 1 | 611 | predicted protein [Chlamydomonas reinhardtii] |
| RefSeq | XP_002178042.1 | 0 | 46 | 597 | 1 | 540 | predicted protein [Phaeodactylum tricornutum CCAP 1055/1] |
| RefSeq | YP_002297804.1 | 0 | 46 | 604 | 2 | 533 | alcohol dehydrogenase (acceptor) [Rhodospirillum centenum SW] |
| RefSeq | ZP_05043291.1 | 0 | 45 | 599 | 2 | 531 | GMC oxidoreductase family [Alcanivorax sp. DG881] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3fim_B | 0 | 45 | 597 | 2 | 563 | B Chain B, Crystal Structure Of Aryl-Alcohol-Oxidase From Pleurotus Eryingii |
| PDB | 3ljp_B | 0 | 45 | 603 | 13 | 537 | A Chain A, Crystal Structure Of Choline Oxidase V464a Mutant |
| PDB | 3ljp_A | 0 | 45 | 603 | 13 | 537 | A Chain A, Crystal Structure Of Choline Oxidase V464a Mutant |
| PDB | 3nne_H | 0 | 45 | 603 | 13 | 537 | A Chain A, Crystal Structure Of Choline Oxidase S101a Mutant |
| PDB | 3nne_G | 0 | 45 | 603 | 13 | 537 | A Chain A, Crystal Structure Of Choline Oxidase S101a Mutant |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| FD836615 | 268 | 107 | 374 | 0 |
| BI724929 | 218 | 263 | 480 | 0 |
| FD835421 | 246 | 188 | 433 | 0 |
| FD820451 | 231 | 256 | 486 | 0 |
| BI717931 | 175 | 1 | 175 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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