| Basic Information | |
|---|---|
| Species | Chlamydomonas reinhardtii |
| Cazyme ID | Cre13.g570700.t1.3 |
| Family | GH18 |
| Protein Properties | Length: 670 Molecular Weight: 69293.1 Isoelectric Point: 7.281 |
| Chromosome | Chromosome/Scaffold: 13 Start: 1265447 End: 1272096 |
| Description | Glycosyl hydrolase family protein with chitinase insertion domain |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH18 | 22 | 354 | 0 |
| HVGLYFPGWFQTTKPNGAFYIQPDTIDLSGVTHVYYAFAWINEGPDFGLRDEFDNLGVVMGLRRRYPGMRALISIGGGGFDTSIWSAATGSAAARAKFVA GVAEWVARVDADGVDLDWEFPGKSDRANMVLLATELRLALDALGPRDGGGGAGPQRRLLTAAVPGYSGDMSGLDLASLAAQLDYFNIMSYELADPCYGDD VTGLHTGWTQVAAGLAAYGSVPANKLVLGLGFYGHGFTLVDAEQWRYPAPSLVTDSSCSRQDTPSYKQLMADLSSSGGSVFVEVAEKAAYYVRGRRWVGF DIPETLWMKQQAAAQRGMAGVMIWDVTLDSDQQ | |||
| Full Sequence |
|---|
| Protein Sequence Length: 670 Download |
| MAFPALGLTL LLLIVQAAVA THVGLYFPGW FQTTKPNGAF YIQPDTIDLS GVTHVYYAFA 60 WINEGPDFGL RDEFDNLGVV MGLRRRYPGM RALISIGGGG FDTSIWSAAT GSAAARAKFV 120 AGVAEWVARV DADGVDLDWE FPGKSDRANM VLLATELRLA LDALGPRDGG GGAGPQRRLL 180 TAAVPGYSGD MSGLDLASLA AQLDYFNIMS YELADPCYGD DVTGLHTGWT QVAAGLAAYG 240 SVPANKLVLG LGFYGHGFTL VDAEQWRYPA PSLVTDSSCS RQDTPSYKQL MADLSSSGGS 300 VFVEVAEKAA YYVRGRRWVG FDIPETLWMK QQAAAQRGMA GVMIWDVTLD SDQQLLRAVT 360 AGGPPPPRPC GGGWGGNGTC VGASAGQCCG EMGYCGLSEE ACGSGCRGGP CAGGEQSPPP 420 PPRGPLCGGG KINSGQCANS KECCSQYGYC GTGREWCGEG CKGGLCWSSP SPAPSPSPKP 480 SPSPSPSPSP SPKPSPSPSP SPSPSSSPSP SPSPKPVPSP KPSPSPKPSP SPKPKPKKSP 540 KPQPAPKPRP SPKRRPSPSP SPSAAEQDAM CGDGRAGRCL NADECCSFFG YCGTTIDHCG 600 NGCQGGACLR MPPPSPGGAG AMCGNGHIND GRCMFSKECC SQYGYCGTTD DHCGQGCQGG 660 ACKQQQPGR* 720 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| COG3325 | ChiA | 5.0e-25 | 20 | 359 | 416 | + Chitinase [Carbohydrate transport and metabolism] | ||
| cd06548 | GH18_chitinase | 2.0e-30 | 23 | 352 | 380 | + The GH18 (glycosyl hydrolases, family 18) type II chitinases hydrolyze chitin, an abundant polymer of N-acetylglucosamine and have been identified in bacteria, fungi, insects, plants, viruses, and protozoan parasites. The structure of this domain is an eight-stranded alpha/beta barrel with a pronounced active-site cleft at the C-terminal end of the beta-barrel. | ||
| cd02872 | GH18_chitolectin_chitotriosidase | 4.0e-39 | 26 | 352 | 369 | + This conserved domain family includes a large number of catalytically inactive chitinase-like lectins (chitolectins) including YKL-39, YKL-40 (HCGP39), YM1, oviductin, and AMCase (acidic mammalian chitinase), as well as catalytically active chitotriosidases. The conserved domain is an eight-stranded alpha/beta barrel fold belonging to the family 18 glycosyl hydrolases. The fold has a pronounced active-site cleft at the C-terminal end of the beta-barrel. The chitolectins lack a key active site glutamate (the proton donor required for hydrolytic activity) but retain highly conserved residues involved in oligosaccharide binding. Chitotriosidase is a chitinolytic enzyme expressed in maturing macrophages, which suggests that it plays a part in antimicrobial defense. Chitotriosidase hydrolyzes chitotriose, as well as colloidal chitin to yield chitobiose and is therefore considered an exochitinase. Chitotriosidase occurs in two major forms, the large form being converted to the small form by either RNA or post-translational processing. Although the small form, containing the chitinase domain alone, is sufficient for the chitinolytic activity, the additional C-terminal chitin-binding domain of the large form plays a role in processing colloidal chitin. The chitotriosidase gene is nonessential in humans, as about 35% of the population are heterozygous and 6% homozygous for an inactivated form of the gene. HCGP39 is a 39-kDa human cartilage glycoprotein thought to play a role in connective tissue remodeling and defense against pathogens. | ||
| smart00636 | Glyco_18 | 9.0e-43 | 26 | 350 | 348 | + Glyco_18 domain. | ||
| pfam00704 | Glyco_hydro_18 | 8.0e-44 | 23 | 350 | 344 | + Glycosyl hydrolases family 18. | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| RefSeq | XP_001693471.1 | 0.00000003 | 384 | 466 | 44 | 116 | predicted protein [Chlamydomonas reinhardtii] |
| RefSeq | XP_001693471.1 | 0.000007 | 437 | 462 | 2 | 27 | predicted protein [Chlamydomonas reinhardtii] |
| RefSeq | XP_001693471.1 | 0 | 565 | 669 | 28 | 123 | predicted protein [Chlamydomonas reinhardtii] |
| RefSeq | XP_002482776.1 | 2e-34 | 52 | 360 | 207 | 526 | bacteriodes thetaiotaomicron symbiotic chitinase, putative [Talaromyces stipitatus ATCC 10500] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1wb0_A | 6e-30 | 26 | 350 | 6 | 342 | A Chain A, Membrane-Embedded Monofunctional Glycosyltransferase Waaa Of Aquifex Aeolicus, Substrate-Free Form |
| PDB | 1waw_A | 6e-30 | 26 | 350 | 6 | 342 | A Chain A, Specificity And Affinity Of Natural Product Cyclopentapeptide Inhibitor Argadin Against Human Chitinase |
| PDB | 1hkm_A | 2e-28 | 26 | 350 | 6 | 342 | A Chain A, Specificity And Affinity Of Natural Product Cyclopentapeptide Inhibitor Argadin Against Human Chitinase |
| PDB | 1hkj_A | 2e-28 | 26 | 350 | 6 | 342 | A Chain A, Specificity And Affinity Of Natural Product Cyclopentapeptide Inhibitor Argadin Against Human Chitinase |
| PDB | 1hki_A | 2e-28 | 26 | 350 | 6 | 342 | A Chain A, Crystal Structure Of Human Chitinase In Complex With Glucoallosamidin B |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| chitin degradation II | 3.2.1.14-RXN | EC-3.2.1.14 | chitinase |
| chitin degradation II | RXN-12623 | EC-3.2.1.14 | chitinase |
| chitin degradation II | RXN-12624 | EC-3.2.1.14 | chitinase |
| chitin degradation III (carnivorous plants) | 3.2.1.14-RXN | EC-3.2.1.14 | chitinase |
