PlantCAZyme

Basic Information
Species	Chlamydomonas reinhardtii
Cazyme ID	Cre13.g574900.t1.2
Family	GT48
Protein Properties	Length: 2500 Molecular Weight: 391320 Isoelectric Point: 7.5123
Chromosome	Chromosome/Scaffold: 13 Start: 1777367 End: 1792924
Description	glucan synthase-like 7
View CDS

External Links
NCBI Taxonomy
CAZyDB

Signature Domain Download full data set without filtering

Family	Start	End	Evalue
GT48	2582	3328	0
PNSEEAQRILGFFINSLGHPSLDKPPSLDKMWSWSILTPLYEEDVMYALDSKALAKETGLKMRKMTDLLGETDDSISLMSYLKAMFPQEWSNFKERMKTL NPDINVKDLSEHDFAPGCDMYEFKLELQMWASLRGQLLARTVHGMMLNEKALDELARLENPQPPNMTELEYKRYIHQLTSCKFEYVVTPQTYGKNRLSKD LRLKWLASSIDILMGKYPRLKVAFLDNADSDNGPAQYSVMARGRDLNDPGQLQHLSDMGIQENEDGVIEVYRVRLPHNKYSGRGVIIGEGKPENQNHAVI FAFGEGLQAIDMNQDNVLAECFKSRNLLSELLPSTKGEFHLFADDDEEVAITRKTIASELMYVMRCRQVQCTYTALVGFREWIFSEKSGALGRFAAATEY AFGTITQRTLTHPARMRLHYGHPDLFNKMFVMTRGGISKATRQLHLTEDVFCGCNHTLRGGRIRYKEFISCGKGRDMGFDSINGFNFKIAGGGGEWAISR ESSRLGSRLDFFRLLMFYHSCIGFYINSWLTTQAAFWNIYALLVFNMAKASHMSDMLQRIYNVQQILQLGTLAMIPYIGQLILEMGIVKAVVIVMQQILT GSLFFYMFQQQTVAQSFMADMTYGSAKYVGTGRGFNIQALDFVKIFTLYTRSHLYYAFELLFMLVSMYCVKGCEVCNYGSLTWSGWLLGFVLIFAPLWFN PFSFDIAKVQVNFLAWQRWMHGDVDTMTGSNWYTWNAGQLEKLRNDN

Full Sequence
Protein Sequence Length: 2500 Download
MPVHFPSNQP PQQNGGGGGG EGSVWENLRA MLGGNSFSED QQMRLAPPQT QQSHQAPQQY 60 AGNYQQQPYQ QQYQQPYQAT PQQHHFAAPA APKSGLSGFL RPFSGKHGNE VLKLGSTPEE 120 AAVAQPNAKD WNTFVQGWLE DDNDPVSVEQ FNGRVNFATN NALLENAERQ EHGPATIGGP 180 GQPVPQGQRS RKSAIRGVSA APKGRAKSAF RRPGAVSAKP VALAPEEDEE MPEEKGIIKK 240 KKKSKRDKKN PFIMPSLHTF AFEHLVHNIV WRVGRNFGFQ AFNINPATRN RGNTAEWTPA 300 SIFVASDHLV QKLVRNYFIR REKGEDERQE ERFAKAVFEL HTTIFYSFEE HWTHMHGLSI 360 RPQVMQDVLD RSRFCDETVV CGMLTELALY YLIYTESSSM RHTPELLWFI YWCCNHSYVM 420 QDLWSRDPPE NFPNIREVRI GLRNKHQALI RELQHTLGIY PDRIRPEDCG RLGPIMSRLK 480 GSDVPEDQRI IIADLIAFGD GNFYYERVVT PIFYVISYEV DHLSNLGVDV AFRLGYDDIN 540 ESMAHTGIVR LALRDLRVRG EQILRGEVND SYGTITRLGY KRGTAETHFD PQVAADWWRT 600 NVFVKTYRER RTWAAVFRAY YRVIAAHLVL YHGMQAQAFA GWNYRLISSC LITHAFCTAF 660 ERFCNWYMTR HPPEPLQTAL SKVFDKKGQF RITRRGAQAL AQARAEGFGE NKAIGADKSR 720 LQPVRVVELE GSPYLGIFGF VEWVMLAFFM CGWYVLQYYD GPFRNFCHDY WPLFSWCYIG 780 AYVGHFILTT RDGYVISLTH ALNLGPLAKA SSSRPDPINW IYGTMHMKWK LFRLNALFWI 840 IAFAMKVPFD YYIICLPSVE PLKLTFAVNW LECPRDHPRY WGVIPCVGGD WVLAFVRLAP 900 FVIVILLDTS LFYQVTTTLF GLFRGLFKLD LGVLTAWEEV VKEFYKAPYR WWFRCMSESG 960 NRNQLTLLKM MLFDNAADPE TGAIVSDGRM FKKAVLTPEE IMGKQKKVVN TQQALDQAGA 1020 NDDDSAAAAG GQGPRGVPGK GGQGGAGKGA DGKPLSQGWK MALQNFGMGG GAGAGGNAGG 1080 GGKIVAPKGG PMLPTSTNMK QMQQQLNKMQ RLPQKLPMTT ITERMIATTE KKDNQMAAEG 1140 KPTLASVAEK LLGNKAAAQA AQQLKTMTPE EKRKEIVARI QAAQMQRKAP GAAVPDDGPS 1200 GAGLPSGDMM AGGGGGLSAG VMVNASKDAP PMQPGGRWGR ALGAFRQRRS SAGDAPDGEG 1260 SGDKAETRRD TLTQVAGEDV PLAKPDAEEV LRPFSGAAAS RKALVPRGSA GEDGVKADAA 1320 LEDADVDDDK DKPASAGSAA TVATLERAGV LGPGFTAAGS SSMPRVGTPT LMGARVSAGG 1380 APPEAVGLPP SMAGLQPSAS MLRGAPATGN ARMSTNGIAS PPAAMAVASA DAPPRYSWTG 1440 APSDAEAMGR QLSVNRAAAE TAAAARTTGS AVASPTTGYR PTRLSSGPKE AAGSGMSVTG 1500 RLSDMGELLG PAGRAALASG GDDTKLQVVN EDDDDLDGGY QGYQRVFSEG KESMDGGAAG 1560 GSGAGSRMAS VAPSPVPMRL PSPLLASGAA AAPLRNSSGA SGSGVGAPAA AKADGVVRAV 1620 SLSQSDHADL NDFLNEVEAA ESAPPRTAGS NGSGGARLSA TGGSNGSGGA RLSATGGRLS 1680 ATGTRLSGAG AGSSGSNKPG PVAEGLEPVA AADVAERLTS NLATAAGEWS KLLHSGMGPL 1740 VTIAGKWVQP KPVIEEVEEE EEETTSGSSD AQPAEGEAME EESEEGSEEE SEEEAGWWER 1800 RVQASERRQQ TGDAMAEEKV LASMTAFRAD GEPVAMAYEW RVEEAEAYDD GESSPRAAAA 1860 ARRAAQALAA LEGVEMSEIA PADTNPAVAS PADGSPTPRA RARAHLQRTI SSASSAAGDS 1920 HKHLRVHASA HAAAVASSAG AADSAAWAAV AAAAKRHGPV AEARELAFAP DVDGGDDTPT 1980 RKRAQAAGMD PALVHGLSRK REGEEGAAGG PQVVRSVSGT SLHSNFFKDA KDEDAESASP 2040 SVTAGAPKRH IRIAAGDGNS PSGAAAGGSK TSGHSSKKRG VAFGGEEVEE YDVNSGPGQI 2100 GLPPSTNAAD SPAGGAGGKA GGDGIMSRWL NALGGNTSNK VAPSPSGNGP IDDVEDEEAA 2160 VRAAARMAGG SGSTHARPKS AFRTSNTSHG HDKHGSHKEG GGISFKLGNS SQRPTTAGQG 2220 RPTTALPRSV QGRTHAKNSS HGESSFVEPG GARPTTALRP TTALRATTAR PTTALLRPMT 2280 ALLGGDDPHK AAKRREKKER EQAIERAADG LTRQHSLVIL DGADAEEDEE IDEVHAQMMM 2340 WNAFAQAWDK IIEDLREADH INNAEKEMLS FVRLDMGSRG HGLRPILLPT FFYAGQVRKV 2400 VDTGQVSVAQ IMVLNELRVL VVWLGCQVGL LSGKHAHVIT SAPFYRGNIN VKHALLRKKM 2460 LQCGLKLVDQ LGEICERHEV PFDMKDIADN LYNLWVALEG

Full Sequence

Protein Sequence Length: 2500 Download

MPVHFPSNQP PQQNGGGGGG EGSVWENLRA MLGGNSFSED QQMRLAPPQT QQSHQAPQQY    60
AGNYQQQPYQ QQYQQPYQAT PQQHHFAAPA APKSGLSGFL RPFSGKHGNE VLKLGSTPEE    120
AAVAQPNAKD WNTFVQGWLE DDNDPVSVEQ FNGRVNFATN NALLENAERQ EHGPATIGGP    180
GQPVPQGQRS RKSAIRGVSA APKGRAKSAF RRPGAVSAKP VALAPEEDEE MPEEKGIIKK    240
KKKSKRDKKN PFIMPSLHTF AFEHLVHNIV WRVGRNFGFQ AFNINPATRN RGNTAEWTPA    300
SIFVASDHLV QKLVRNYFIR REKGEDERQE ERFAKAVFEL HTTIFYSFEE HWTHMHGLSI    360
RPQVMQDVLD RSRFCDETVV CGMLTELALY YLIYTESSSM RHTPELLWFI YWCCNHSYVM    420
QDLWSRDPPE NFPNIREVRI GLRNKHQALI RELQHTLGIY PDRIRPEDCG RLGPIMSRLK    480
GSDVPEDQRI IIADLIAFGD GNFYYERVVT PIFYVISYEV DHLSNLGVDV AFRLGYDDIN    540
ESMAHTGIVR LALRDLRVRG EQILRGEVND SYGTITRLGY KRGTAETHFD PQVAADWWRT    600
NVFVKTYRER RTWAAVFRAY YRVIAAHLVL YHGMQAQAFA GWNYRLISSC LITHAFCTAF    660
ERFCNWYMTR HPPEPLQTAL SKVFDKKGQF RITRRGAQAL AQARAEGFGE NKAIGADKSR    720
LQPVRVVELE GSPYLGIFGF VEWVMLAFFM CGWYVLQYYD GPFRNFCHDY WPLFSWCYIG    780
AYVGHFILTT RDGYVISLTH ALNLGPLAKA SSSRPDPINW IYGTMHMKWK LFRLNALFWI    840
IAFAMKVPFD YYIICLPSVE PLKLTFAVNW LECPRDHPRY WGVIPCVGGD WVLAFVRLAP    900
FVIVILLDTS LFYQVTTTLF GLFRGLFKLD LGVLTAWEEV VKEFYKAPYR WWFRCMSESG    960
NRNQLTLLKM MLFDNAADPE TGAIVSDGRM FKKAVLTPEE IMGKQKKVVN TQQALDQAGA    1020
NDDDSAAAAG GQGPRGVPGK GGQGGAGKGA DGKPLSQGWK MALQNFGMGG GAGAGGNAGG    1080
GGKIVAPKGG PMLPTSTNMK QMQQQLNKMQ RLPQKLPMTT ITERMIATTE KKDNQMAAEG    1140
KPTLASVAEK LLGNKAAAQA AQQLKTMTPE EKRKEIVARI QAAQMQRKAP GAAVPDDGPS    1200
GAGLPSGDMM AGGGGGLSAG VMVNASKDAP PMQPGGRWGR ALGAFRQRRS SAGDAPDGEG    1260
SGDKAETRRD TLTQVAGEDV PLAKPDAEEV LRPFSGAAAS RKALVPRGSA GEDGVKADAA    1320
LEDADVDDDK DKPASAGSAA TVATLERAGV LGPGFTAAGS SSMPRVGTPT LMGARVSAGG    1380
APPEAVGLPP SMAGLQPSAS MLRGAPATGN ARMSTNGIAS PPAAMAVASA DAPPRYSWTG    1440
APSDAEAMGR QLSVNRAAAE TAAAARTTGS AVASPTTGYR PTRLSSGPKE AAGSGMSVTG    1500
RLSDMGELLG PAGRAALASG GDDTKLQVVN EDDDDLDGGY QGYQRVFSEG KESMDGGAAG    1560
GSGAGSRMAS VAPSPVPMRL PSPLLASGAA AAPLRNSSGA SGSGVGAPAA AKADGVVRAV    1620
SLSQSDHADL NDFLNEVEAA ESAPPRTAGS NGSGGARLSA TGGSNGSGGA RLSATGGRLS    1680
ATGTRLSGAG AGSSGSNKPG PVAEGLEPVA AADVAERLTS NLATAAGEWS KLLHSGMGPL    1740
VTIAGKWVQP KPVIEEVEEE EEETTSGSSD AQPAEGEAME EESEEGSEEE SEEEAGWWER    1800
RVQASERRQQ TGDAMAEEKV LASMTAFRAD GEPVAMAYEW RVEEAEAYDD GESSPRAAAA    1860
ARRAAQALAA LEGVEMSEIA PADTNPAVAS PADGSPTPRA RARAHLQRTI SSASSAAGDS    1920
HKHLRVHASA HAAAVASSAG AADSAAWAAV AAAAKRHGPV AEARELAFAP DVDGGDDTPT    1980
RKRAQAAGMD PALVHGLSRK REGEEGAAGG PQVVRSVSGT SLHSNFFKDA KDEDAESASP    2040
SVTAGAPKRH IRIAAGDGNS PSGAAAGGSK TSGHSSKKRG VAFGGEEVEE YDVNSGPGQI    2100
GLPPSTNAAD SPAGGAGGKA GGDGIMSRWL NALGGNTSNK VAPSPSGNGP IDDVEDEEAA    2160
VRAAARMAGG SGSTHARPKS AFRTSNTSHG HDKHGSHKEG GGISFKLGNS SQRPTTAGQG    2220
RPTTALPRSV QGRTHAKNSS HGESSFVEPG GARPTTALRP TTALRATTAR PTTALLRPMT    2280
ALLGGDDPHK AAKRREKKER EQAIERAADG LTRQHSLVIL DGADAEEDEE IDEVHAQMMM    2340
WNAFAQAWDK IIEDLREADH INNAEKEMLS FVRLDMGSRG HGLRPILLPT FFYAGQVRKV    2400
VDTGQVSVAQ IMVLNELRVL VVWLGCQVGL LSGKHAHVIT SAPFYRGNIN VKHALLRKKM    2460
LQCGLKLVDQ LGEICERHEV PFDMKDIADN LYNLWVALEG 

Functional Domains Download unfiltered results here

Cdd ID	Domain	E-Value	Start	End	Length	Domain Description
pfam14288	FKS1_dom1	0.004	498	541	44	+ 1,3-beta-glucan synthase subunit FKS1, domain-1. The FKS1_dom1 domain is likely to be the 'Class I' region just N-terminal to the first set of transmembrane helices that is involved in 1,3-beta-glucan synthesis itself. This family is found on proteins with family Glucan_synthase, pfam02364.
TIGR02185	Trep_Strep	0.002	3336	3512	189	+ conserved hypothetical integral membrane protein TIGR02185. This family consists of strongly hydrophobic proteins about 190 amino acids in length with a strongly basic motif near the C-terminus. If is found in rather few species, but in paralogous families of 12 members in the oral pathogenic spirochaete Treponema denticola and 2 in Streptococcus pneumoniae R6 [Cell envelope, Other].
pfam14288	FKS1_dom1	6.0e-6	383	415	34	+ 1,3-beta-glucan synthase subunit FKS1, domain-1. The FKS1_dom1 domain is likely to be the 'Class I' region just N-terminal to the first set of transmembrane helices that is involved in 1,3-beta-glucan synthesis itself. This family is found on proteins with family Glucan_synthase, pfam02364.
pfam02364	Glucan_synthase	4.0e-146	2582	3316	785	+ 1,3-beta-glucan synthase component. This family consists of various 1,3-beta-glucan synthase components including Gls1, Gls2 and Gls3 from yeast. 1,3-beta-glucan synthase EC:2.4.1.34 also known as callose synthase catalyzes the formation of a beta-1,3-glucan polymer that is a major component of the fungal cell wall. The reaction catalyzed is:- UDP-glucose + {(1,3)-beta-D-glucosyl}(N) <=> UDP + {(1,3)-beta-D-glucosyl}(N+1).

Annotations - NR Download unfiltered results here
Source	Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
RefSeq	NP_172136.1	0	2328	3361	808	1765	ATGSL07 (glucan synthase-like 7); 1,3-beta-glucan synthase/ transferase, transferring glycosyl groups [Arabidopsis thaliana]
RefSeq	XP_001693159.1	0	238	793	136	711	1,3-beta-D-glucan synthase [Chlamydomonas reinhardtii]
RefSeq	XP_001693160.1	3e-21	911	1090	2	189	glycosyl transferase [Chlamydomonas reinhardtii]
RefSeq	XP_001693160.1	0	2277	3526	627	1891	glycosyl transferase [Chlamydomonas reinhardtii]
RefSeq	XP_001693763.1	0	1	3592	1	3562	flagellar associated protein, callose synthase-like protein [Chlamydomonas reinhardtii]

Metabolic Pathways
Pathway Name	Reaction	EC	Protein Name
callose biosynthesis	13-BETA-GLUCAN-SYNTHASE-RXN	EC-2.4.1.34	1,3-β-glucan synthase

Transmembrane Domains

Start	End
638	660
734	756
771	788
831	853
3106	3128
3141	3163
3167	3189
3234	3251
3261	3283
3335	3357
3377	3399
3412	3434
3444	3466
3486	3508

EST Download unfiltered results here

Hit	Length	Start	End	EValue
BI721565	182	3273	3454	0
BM001542	191	3350	3540	0
AV640595	173	2856	3028	0
BM003345	158	3273	3430	0
BM002938	161	3281	3441	0

Orthologous Group
Species	ID
Chlamydomonas reinhardtii	Cre03.g198200.t1.3	Cre03.g185350.t1.2	Cre03.g185000.t1.1	Cre04.g214650.t1.3
Volvox carteri	Vocar20005515m	Vocar20008000m	Vocar20010016m

CAZyme Information