| Basic Information | |
|---|---|
| Species | Chlamydomonas reinhardtii |
| Cazyme ID | Cre13.g582250.t1.2 |
| Family | GH85 |
| Protein Properties | Length: 1219 Molecular Weight: 121719 Isoelectric Point: 7.8681 |
| Chromosome | Chromosome/Scaffold: 13 Start: 2704451 End: 2713215 |
| Description | Glycosyl hydrolase family 85 |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH85 | 182 | 439 | 0 |
| MMGGYLADRLVQGGADPGFFRLWQWEQVDVFVYFSHHLVTLPPPGWTAAAHRNGVRVLGTFITEWEEGRRRCEALFASPAAARAAADQLVALAVHHGFEG WLVNVENGLGRELVPHLTHFVAHLRARMAAEVGPHALVIWYDAITTEGRLAWQDGLTRLNAPFFDAADALFVNYRWQEGAPAAVAAAAGSRAAEVYMGVD VFGRGSYGGGKENCPVALAAARAAGLSAALFAPGWVYEEFDRATFELRQEAFWAAIRG | |||
| Full Sequence |
|---|
| Protein Sequence Length: 1219 Download |
| MKRRGFIAVL LIGVGTGVWW AWRNRKVRER VQNLAKEVVR RSHTVRELVR KVFKPQVASE 60 AAANEAARIV TGLDAPEVRP LTTVDQLLRW SPDPAADPDA PFCRTYNSPL LLPPAPPVPA 120 TGPVPPTPTA TTSTSATAST TTTTTTTIAA AAPATAGAPA TAAGATAATA ASRPRLLVCH 180 DMMGGYLADR LVQGGADPGF FRLWQWEQVD VFVYFSHHLV TLPPPGWTAA AHRNGVRVLG 240 TFITEWEEGR RRCEALFASP AAARAAADQL VALAVHHGFE GWLVNVENGL GRELVPHLTH 300 FVAHLRARMA AEVGPHALVI WYDAITTEGR LAWQDGLTRL NAPFFDAADA LFVNYRWQEG 360 APAAVAAAAG SRAAEVYMGV DVFGRGSYGG GKENCPVALA AARAAGLSAA LFAPGWVYEE 420 FDRATFELRQ EAFWAAIRGV WPARPALVAR LPLQSHFNTG AGRALWLEGR RVSAAPWFNL 480 SAQDVVASST PLPSAAATSS SAATTPASTA APTSLQPPQP KGATVLPPAA VSPLTVRPTS 540 QLAYTGGACL ALTAPPPPPP SLSSAGPWVA LHALYSAALP LDPGFGSGSG IIGIGSGSGL 600 DLTWTAACAG SSRVGLLLRT APAEVAGSAG CCVGLAVGPW GAGDKAVVEA LRKAGYTCAA 660 CPPGAATGAV TCPLSDATTT TAASAAAAAV ASGPAVPSGL PWVTYSAQLP RRLFPASGSG 720 SSGSSSSSTA AAGSSRSSSS SSSRGAITAV GIVCYHSATA GSGAQTATAG AGAATATAKA 780 GYFGGGEPLG PSAPFTAFLG LLSIREHRGS PTDPPVPATA TVAAPSSAAG VATAPIPVAP 840 APPPATPPPP LTGAAVSRVR WARPTARAAA GAASSDASAA AAAASPASAL LGGLGLGGPR 900 LSGELVHKDD LLSPTTSGSA GAAAAAAAAA AAGKAHAAAA AAAVTEEDDD WERGSSGGGG 960 AGAGAGARNG AGASGAAGSS LAGLESPIKI KLPTLLGDDE EDEEEEEEAA GGARARARRA 1020 GAGVEEEDDG TQLLTCELSW APQFEAGGAP RHRSYHVWVH FNRQEAPAGG AAASGGRGVV 1080 PLPGIASPTG PSALGALGVA AAAGAAGADA AGGRKGAGSG SGKAASKQQK LAAASEAAAA 1140 AAVDKDSTWE VLHPDWSGAV WVGEAHVARF RLVQLEVPGW ARSVSLLAQP AAADSGALGA 1200 LQPLACVPVP VPARRDRG* 1260 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| COG4724 | COG4724 | 1.0e-20 | 201 | 486 | 319 | + Endo-beta-N-acetylglucosaminidase D [Carbohydrate transport and metabolism] | ||
| pfam03644 | Glyco_hydro_85 | 1.0e-75 | 182 | 464 | 302 | + Glycosyl hydrolase family 85. Family of endo-beta-N-acetylglucosaminidases. These enzymes work on a broad spectrum of substrates. | ||
| cd06547 | GH85_ENGase | 1.0e-88 | 177 | 485 | 329 | + Endo-beta-N-acetylglucosaminidase (ENGase) hydrolyzes the N-N'-diacetylchitobiosyl core of N-glycosylproteins. The beta-1,4-glycosyl bond located between two N-acetylglucosamine residues is hydrolyzed such that N-acetylglucosamine 1 remains with the protein and N-acetylglucosamine 2 forms the reducing end of the released glycan. ENGase is a key enzyme in the processing of free oligosaccharides in the cytosol of eukaryotes. Oligosaccharides formed in the lumen of the endoplasmic reticulum are transported into the cytosol where they are catabolized by cytosolic ENGases and other enzymes, possibly to maximize the reutilization of the component sugars. ENGases have an eight-stranded alpha/beta barrel topology and are classified as a family 85 glycosyl hydrolase (GH85) domain. The GH85 ENGases are sequence-similar to the family 18 glycosyl hydrolases, also known as GH18 chitinases. An ENGase-like protein is also found in bacteria and is included in this alignment model. | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| DDBJ | BAB09989.1 | 0 | 171 | 483 | 60 | 371 | unnamed protein product [Arabidopsis thaliana] |
| RefSeq | NP_187715.1 | 0 | 172 | 483 | 66 | 376 | glycosyl hydrolase family 85 protein [Arabidopsis thaliana] |
| RefSeq | NP_196165.3 | 0 | 171 | 485 | 60 | 373 | hydrolase, acting on glycosyl bonds / mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase [Arabidopsis thaliana] |
| RefSeq | XP_002273683.1 | 0 | 172 | 483 | 62 | 372 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002315138.1 | 0 | 172 | 483 | 67 | 377 | predicted protein [Populus trichocarpa] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2vtf_B | 2e-24 | 85 | 486 | 19 | 394 | A Chain A, X-Ray Crystal Structure Of The Endo-Beta-N- Acetylglucosaminidase From Arthrobacter Protophormiae E173q Mutant Reveals A Tim Barrel Catalytic Domain And Two Ancillary Domains |
| PDB | 2vtf_A | 2e-24 | 85 | 486 | 19 | 394 | A Chain A, X-Ray Crystal Structure Of The Endo-Beta-N- Acetylglucosaminidase From Arthrobacter Protophormiae E173q Mutant Reveals A Tim Barrel Catalytic Domain And Two Ancillary Domains |
| PDB | 3fha_D | 3e-24 | 85 | 486 | 14 | 389 | A Chain A, Structure Of Endo-Beta-N-Acetylglucosaminidase A |
| PDB | 3fha_C | 3e-24 | 85 | 486 | 14 | 389 | A Chain A, Structure Of Endo-Beta-N-Acetylglucosaminidase A |
| PDB | 3fha_B | 3e-24 | 85 | 486 | 14 | 389 | A Chain A, Structure Of Endo-Beta-N-Acetylglucosaminidase A |
