| Basic Information | |
|---|---|
| Species | Chlamydomonas reinhardtii |
| Cazyme ID | Cre17.g719900.t1.2 |
| Family | CBM20 |
| Protein Properties | Length: 1245 Molecular Weight: 130607 Isoelectric Point: 7.7816 |
| Chromosome | Chromosome/Scaffold: 17 Start: 2934922 End: 2944603 |
| Description | catalytics;carbohydrate kinases;phosphoglucan, water dikinases |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| CBM20 | 43 | 124 | 5.7e-27 |
| VPVDFSIKCKVDFGESVRLVGNQPFLGSWQPKKAVELRWTEGNIWKTEARVPVGTEVEFKVVKMKPDGEVIWEDGDNRTFKV | |||
| Full Sequence |
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| Protein Sequence Length: 1245 Download |
| MQALQTGRSP ALHGASQRRN PGVLRKTWSR VDAVRVQAAE SSVPVDFSIK CKVDFGESVR 60 LVGNQPFLGS WQPKKAVELR WTEGNIWKTE ARVPVGTEVE FKVVKMKPDG EVIWEDGDNR 120 TFKVLGPDYG LNVISAWAKA QDTKIDAFKL SDGSRQAPAA AAAAASNGAG AAAQQPPQQQ 180 SSSKSSRSRE ASATSSVDGG DSSSSSRGST AVLSKSSFEE ESGSRGAWVG AGPEFVRSRK 240 KDERPAGTWR TEGLSGVALA LVKGDEQAAS WLKKLGLQKQ LLVDSPPACR PRLDDLSYLY 300 VYATWVNTGA LACAESGSHY RPNHHANLAL AMFRSLEWVI GDATRGGGGR SAAAEKLIMA 360 ARRMHTRLPS FSGEFRVSTP LTRIRDIAHR NDIPKELKDE IKHTLQNKLH RSAGPEDLVA 420 TEVMLQRVTA RPGEYSEAFV EEFKIFTREL REFFNASGLV ELLQGTLPAL DDAHGSAVRS 480 LLQLKERVEA AGGGNGNGGG GGAKLDDLMA LLTATTQARS FYSAGLVAGL RNDVTDEVLA 540 MRQRWRLADI RLEEYAFVVL SRIIGQLEEQ GAPAKLPKAT NLQWALPLAA LTSGLRHMGL 600 SLYDTRELLV LENELQRWHS TCPLTETRDA ALRAKATLER ALRVATEYSD ALADVYTQPA 660 ATLGRALGLP EHMGGIFAEA EVRASMAFQV SKLAAMLARA LRVAAGQEPW DVLVPGEVSG 720 VLREAASLDS NALGPASSSN GNGADDGVVL VVRRADGDEE LGPLGPRLRG VVLLQELPHL 780 SHLGVRARQD KVTFVTCDDA DVINRVLRPM LGRRVTLTAT GDCAVSLREG GSAAPAPSKA 840 PAAKAAAAPA PAAGSAPDAA AVAAVSKAYA SGKWGEVVVP LDKAVVAACG SKSAKCGALV 900 TLAASSGGLF AAPRGAVLPF GCLEAAVAAA GGDTESRFKE LLGKLEDAKS GDGAALDATC 960 EQLQALVAGL TVPPALVRQL VQSLTGGAAA DPQLLLAVRS SANVEDLAGM SAAGLYDSVV 1020 GVAAADDGAV GAAVAAVWAS LYTRRAVMSR RSAGVSQSSA RMAVLVMEMV APDLSFVLHT 1080 ARPRDGNDKV LLAEVAPGQG ETLASGVRGT PWRFEVEKAS GKVDTLAYAN FSTALMLPAQ 1140 GTAAAVPAFA AYGSGAATSL IAGSGGEARP LPHVPTALVD REVDYSTQRM SADKDWRVAT 1200 VQKLAEVGGS IEKAMGGVPQ DIEGGVVLGR DGALALHVFQ TRPQ* 1260 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| smart01065 | CBM_2 | 1.0e-20 | 43 | 124 | 86 | + Starch binding domain. | ||
| pfam00686 | CBM_20 | 1.0e-22 | 42 | 124 | 86 | + Starch binding domain. | ||
| cd05467 | CBM20 | 5.0e-24 | 45 | 137 | 97 | + The family 20 carbohydrate-binding module (CBM20), also known as the starch-binding domain, is found in a large number of starch degrading enzymes including alpha-amylase, beta-amylase, glucoamylase, and CGTase (cyclodextrin glucanotransferase). CBM20 is also present in proteins that have a regulatory role in starch metabolism in plants (e.g. alpha-amylase) or glycogen metabolism in mammals (e.g. laforin). CBM20 folds as an antiparallel beta-barrel structure with two starch binding sites. These two sites are thought to differ functionally with site 1 acting as the initial starch recognition site and site 2 involved in the specific recognition of appropriate regions of starch. | ||
| PRK06241 | PRK06241 | 1.0e-27 | 882 | 1243 | 363 | + phosphoenolpyruvate synthase; Validated | ||
| pfam01326 | PPDK_N | 1.0e-37 | 913 | 1244 | 332 | + Pyruvate phosphate dikinase, PEP/pyruvate binding domain. This enzyme catalyzes the reversible conversion of ATP to AMP, pyrophosphate and phosphoenolpyruvate (PEP). | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAU93516.1 | 0 | 41 | 1244 | 72 | 1194 | chloroplast alpha-glucan water dikinase isoform 3 [Arabidopsis thaliana] |
| RefSeq | NP_198009.3 | 0 | 41 | 1244 | 72 | 1194 | ATGWD3; carbohydrate kinase/ catalytic/ phosphoglucan, water dikinase [Arabidopsis thaliana] |
| RefSeq | XP_001697099.1 | 0 | 1 | 122 | 1 | 107 | phosphoglucan water dikinase [Chlamydomonas reinhardtii] |
| RefSeq | XP_001697099.1 | 0 | 190 | 824 | 118 | 667 | phosphoglucan water dikinase [Chlamydomonas reinhardtii] |
| RefSeq | XP_001697099.1 | 0 | 893 | 1244 | 668 | 978 | phosphoglucan water dikinase [Chlamydomonas reinhardtii] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2ols_A | 0.000001 | 996 | 1244 | 122 | 336 | A Chain A, The Crystal Structure Of The Phosphoenolpyruvate Synthase From Neisseria Meningitidis |
| PDB | 1kum_A | 0.000003 | 41 | 124 | 5 | 92 | A Chain A, The Crystal Structure Of The Phosphoenolpyruvate Synthase From Neisseria Meningitidis |
| PDB | 1kul_A | 0.000003 | 41 | 124 | 5 | 92 | A Chain A, The Crystal Structure Of The Phosphoenolpyruvate Synthase From Neisseria Meningitidis |
| PDB | 1acz_A | 0.000003 | 41 | 124 | 5 | 92 | A Chain A, Glucoamylase, Granular Starch-Binding Domain Complex With Cyclodextrin, Nmr, 5 Structures |
| PDB | 1ac0_A | 0.000003 | 41 | 124 | 5 | 92 | A Chain A, Glucoamylase, Granular Starch-Binding Domain Complex With Cyclodextrin, Nmr, Minimized Average Structure |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| starch degradation II | RXN-12204 | EC-2.7.9.5 | phosphoglucan, water dikinase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO778000 | 688 | 578 | 1244 | 0 |
| HO778000 | 132 | 358 | 489 | 0 |
| HO778000 | 47 | 525 | 571 | 0 |
| HO794745 | 747 | 525 | 1244 | 0 |
| HO794745 | 45 | 442 | 486 | 0.82 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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