| Basic Information | |
|---|---|
| Species | Cucumis sativus |
| Cazyme ID | Cucsa.033020.1 |
| Family | GT24 |
| Protein Properties | Length: 1579 Molecular Weight: 177758 Isoelectric Point: 6.0419 |
| Chromosome | Chromosome/Scaffold: 00429 Start: 2315935 End: 2342792 |
| Description | UDP-glucose:glycoprotein glucosyltransferases;transferases, transferring hexosyl groups;transferases, transferring glycosyl groups |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT24 | 1329 | 1576 | 0 |
| INIFSIASGHLYERFLKIMILSVLKNTHRPVKFWFIKNYLSPQFKDVIPLMAEEYGFDFELITYKWPTWLHKQKEKQRIIWAYKILFLDVIFPLSLEKVI FVDADQIVRTDMGELYDMDIKGKPLAYTPFCDNNKDMDGYRFWRQGFWKEHLRGKPYHISALYVVDLKKFRETAAGDNLRVFYESLSKDPNSLSNLDQDL PNYAQHTVPIFSLPQEWLWCESWCGNVTKSKAKTIDLCNNPMTKEPKL | |||
| Full Sequence |
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| Protein Sequence Length: 1579 Download |
| MGTSCFRSGC RPLIVVLLLA IYGGNGIFAE IRKPKNVQVA VQAKWSGTSV LLEAGELLAK 60 EQKDLYWEFI EVWLREEGND ADADADAPTA KACLKKILKH GRSLLNEPLA SLYEFSLVLR 120 SASPRLVLYQ QLADESLSSF PLPEENNSNI VGEGNESIER RISDTSVVGL KPKTPDGKCC 180 WVDTGGSLFF DVPELLTWLQ NPAESVGDSI QPPDLYDFDH VHFGSSSGSR LAILYGALGT 240 YCFKQFHDTL VNAAKEGKVK YVVRPVIPSG CELKINSCGA VGARGSLNLG GYGVELALKN 300 MEYKAMDDSA IKKGVTLEDP RTEDLSQEVR GFIFSKILER KPELTSEVMA FRDYLLSSTV 360 SDTLNVWELK DLGHQTAQRI VQASDPLQSM QEISQNFPSI VSSLSRMKLN DSVKDEITAN 420 QRMIPPGKSL MALNGALINI EDVDLYLLID MIHQDLLLAD QFTKLKIPHH TIRKLLSSLP 480 PADSDLLRVD FRSSHVHFLN NLEEDAMYKR WRSNINEILM PVFPGQLRYI RKNLFHAVYV 540 LDPATVCGLQ TIDTILSFYE NNFPIRFGVL LFSSKFIKQT ESKDGELNKS EADTSSLMIQ 600 LFIYLKENQG IQTAFQFLSN VNKLRLEADG LSDDAPEMHH VEGAFVETLL PKSKSPPQDV 660 LLKLEKEQTF KDLAEESSMF TFSLGLSKSE CSLLMNGLVF DSSEESLINA MNEELPRIQE 720 QVYYGHISSR TNVLEKLLSD SGLSRYNPQI IAEGKPRIVS LFPSTHGAES LLNDLNYLHS 780 PGTMDDLKPV THLLIIDAAS KKGIKLLKEG LLYLMRGSKN ARVGLLFTTS NHTSESSLLL 840 AKVFQISASL HSHKMKVLNF LDQLCSVYSQ KFIHESSVAV DSSQEFIEKA CELAEANELP 900 PKAYRIALSD SFFDELKKHF SQVEHLLSGQ LGLESIFNAV ITNGRVTLVT DAISFLSHDL 960 HLLEAIEFKR RIKHIVEIVE EVKWDDFDPD RLTSNFLSDV IMFVSSSMAQ RERSSESARF 1020 EVLNAEYSAI VVDNENASIH IDAVIDPLSP SGQKLSSILR VLSKYIQPNM RIILNPLSSL 1080 VDLPLKNYYR YVLPSVDDFS STDATINGPK AFFANMPLSK TLTMNLDVPE PWLVEPVIAV 1140 HDLDNILLEN IGDTRTLQAV FELEALVLTG HCSEKNQEPP RGLQLILGTK STPHLVDTLV 1200 MANLGYWQMK VSPGVWYLQL APGRSSELYL LKQGGGKSQD TLSKRIIIDD LRGKVVHMEV 1260 EKKKGKENEK LLVPDGGDDL LENKKESHNS WNSNFLKWAT GFIGSNDKSK NTKSTSVDQG 1320 KGGRYGKAIN IFSIASGHLY ERFLKIMILS VLKNTHRPVK FWFIKNYLSP QFKDVIPLMA 1380 EEYGFDFELI TYKWPTWLHK QKEKQRIIWA YKILFLDVIF PLSLEKVIFV DADQIVRTDM 1440 GELYDMDIKG KPLAYTPFCD NNKDMDGYRF WRQGFWKEHL RGKPYHISAL YVVDLKKFRE 1500 TAAGDNLRVF YESLSKDPNS LSNLDQDLPN YAQHTVPIFS LPQEWLWCES WCGNVTKSKA 1560 KTIDLCNNPM TKEPKLQV* 1620 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| pfam01501 | Glyco_transf_8 | 9.0e-7 | 1333 | 1528 | 206 | + Glycosyl transferase family 8. This family includes enzymes that transfer sugar residues to donor molecules. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. This family includes Lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, and glycogenin glucosyltransferase. | ||
| cd04194 | GT8_A4GalT_like | 2.0e-15 | 1329 | 1545 | 227 | + A4GalT_like proteins catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface. The members of this family of glycosyltransferases catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface. The enzymes exhibit broad substrate specificities. The known functions found in this family include: Alpha-1,4-galactosyltransferase, LOS-alpha-1,3-D-galactosyltransferase, UDP-glucose:(galactosyl) LPS alpha1,2-glucosyltransferase, UDP-galactose: (glucosyl) LPS alpha1,2-galactosyltransferase, and UDP-glucose:(glucosyl) LPS alpha1,2-glucosyltransferase. Alpha-1,4-galactosyltransferase from N. meningitidis adds an alpha-galactose from UDP-Gal (the donor) to a terminal lactose (the acceptor) of the LOS structure of outer membrane. LOSs are virulence factors that enable the organism to evade the immune system of host cells. In E. coli, the three alpha-1,2-glycosyltransferases, that are involved in the synthesis of the outer core region of the LPS, are all members of this family. The three enzymes share 40 % of sequence identity, but have different sugar donor or acceptor specificities, representing the structural diversity of LPS. | ||
| cd00505 | Glyco_transf_8 | 3.0e-55 | 1329 | 1576 | 257 | + Members of glycosyltransferase family 8 (GT-8) are involved in lipopolysaccharide biosynthesis and glycogen synthesis. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. GT-8 comprises enzymes with a number of known activities: lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, glycogenin glucosyltransferase, and N-acetylglucosaminyltransferase. GT-8 enzymes contains a conserved DXD motif which is essential in the coordination of a catalytic divalent cation, most commonly Mn2+. | ||
| pfam06427 | UDP-g_GGTase | 1.0e-80 | 998 | 1203 | 212 | + UDP-glucose:Glycoprotein Glucosyltransferase. The N-terminal region of this group of proteins is required for correct folding of the ER UDP-Glc: glucosyltransferase. | ||
| cd06432 | GT8_HUGT1_C_like | 1.0e-173 | 1329 | 1576 | 248 | + The C-terminal domain of HUGT1-like is highly homologous to the GT 8 family. C-terminal domain of glycoprotein glucosyltransferase (UGT). UGT is a large glycoprotein whose C-terminus contains the catalytic activity. This catalytic C-terminal domain is highly homologous to Glycosyltransferase Family 8 (GT 8) and contains the DXD motif that coordinates donor sugar binding, characteristic for Family 8 glycosyltransferases. GT 8 proteins are retaining enzymes based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. The non-catalytic N-terminal portion of the human UTG1 (HUGT1) has been shown to monitor the protein folding status and activate its glucosyltransferase activity. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003980 | UDP-glucose:glycoprotein glucosyltransferase activity |
| GO:0006486 | protein glycosylation |
| GO:0016757 | transferase activity, transferring glycosyl groups |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAG51883.1 | 0 | 1 | 1577 | 1 | 1610 | AC016162_4 putative UDP-glucose:glycoprotein glucosyltransferase; 101200-91134 [Arabidopsis thaliana] |
| RefSeq | NP_177278.3 | 0 | 1 | 1577 | 1 | 1549 | EBS1 (EMS-mutagenized bri1 suppressor 1); UDP-glucose:glycoprotein glucosyltransferase/ transferase, transferring glycosyl groups / transferase, transferring hexosyl groups [Arabidopsis thaliana] |
| RefSeq | XP_002268972.1 | 0 | 1 | 1577 | 1 | 1552 | PREDICTED: similar to EBS1 (EMS-MUTAGENIZED BRI1 SUPPRESSOR 1); UDP-glucose:glycoprotein glucosyltransferase/ transferase, transferring glycosyl groups / transferase, transferring hexosyl groups [Vitis vinifera] |
| RefSeq | XP_002454743.1 | 0 | 21 | 1577 | 28 | 1512 | hypothetical protein SORBIDRAFT_04g036540 [Sorghum bicolor] |
| RefSeq | XP_002529534.1 | 0 | 1 | 1526 | 1 | 1499 | UDP-glucose glycoprotein:glucosyltransferase, putative [Ricinus communis] |
