Basic Information | |
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Species | Cucumis sativus |
Cazyme ID | Cucsa.045150.2 |
Family | PL4 |
Protein Properties | Length: 597 Molecular Weight: 67920.7 Isoelectric Point: 6.3709 |
Chromosome | Chromosome/Scaffold: 00542 Start: 1189878 End: 1193563 |
Description | Rhamnogalacturonate lyase family protein |
View CDS |
External Links |
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NCBI Taxonomy |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
PL4 | 7 | 575 | 0 |
VKSSESKRGYWDINWSWPGGKDRYQLLKGSEFNVINSSDDTIEISFRKDFDLSTRGNKLPLGVDIRYIMRTGIPGFYCYAIYEHPSECRAFDLAQTRMVF KLRQEKFHYMAISDEKQRIMPMPEDLRPGRGEELIVPESVLLVNPINPDLKGEVDDKYQYSEDNKDGGVHGWISSSPNNIGFWIVFPSHEFRNGGPTKQN LTVHTGPTCLAMFHGTHYIGEDILTHIKEGEAWRKVFGPILVYLNSTSDVSEAHNLWIDAKEQRMQEETAWPYNFVASSFYLMARERGSISGRLLVRDRF VSSSPIPARDAHIGLSAAREEGAWQIESKEYQFWVKTDSNGDFTIRNIIPGVYGLHGWVPGFIGDYLHKSLVTVSAGSYNHLGILTYSPPRDGPTVWEIG FPDRTANSFYVPDVNPMYVNKLFLHSPEKFRQYGLWEGYSDLHPRNDQIFTVGINDPKKDWFFAQVCRRGEDGKYVATTWTIKFNMTSLTDGTYRLRLSI ASATRSDLKINVNSMGSESSLVFQLMNLGMDNTVCRHGNHGLYRIYSIDIPSSMLVKGDNCIFLTQARN |
Full Sequence |
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Protein Sequence Length: 597 Download |
MDNLLDVKSS ESKRGYWDIN WSWPGGKDRY QLLKGSEFNV INSSDDTIEI SFRKDFDLST 60 RGNKLPLGVD IRYIMRTGIP GFYCYAIYEH PSECRAFDLA QTRMVFKLRQ EKFHYMAISD 120 EKQRIMPMPE DLRPGRGEEL IVPESVLLVN PINPDLKGEV DDKYQYSEDN KDGGVHGWIS 180 SSPNNIGFWI VFPSHEFRNG GPTKQNLTVH TGPTCLAMFH GTHYIGEDIL THIKEGEAWR 240 KVFGPILVYL NSTSDVSEAH NLWIDAKEQR MQEETAWPYN FVASSFYLMA RERGSISGRL 300 LVRDRFVSSS PIPARDAHIG LSAAREEGAW QIESKEYQFW VKTDSNGDFT IRNIIPGVYG 360 LHGWVPGFIG DYLHKSLVTV SAGSYNHLGI LTYSPPRDGP TVWEIGFPDR TANSFYVPDV 420 NPMYVNKLFL HSPEKFRQYG LWEGYSDLHP RNDQIFTVGI NDPKKDWFFA QVCRRGEDGK 480 YVATTWTIKF NMTSLTDGTY RLRLSIASAT RSDLKINVNS MGSESSLVFQ LMNLGMDNTV 540 CRHGNHGLYR IYSIDIPSSM LVKGDNCIFL TQARNGDPLC GILYDYLRLE APDATP* 600 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
pfam13620 | CarboxypepD_reg | 0.007 | 295 | 384 | 90 | + Carboxypeptidase regulatory-like domain. | ||
cd10316 | RGL4_M | 3.0e-25 | 292 | 389 | 98 | + Middle domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold. Both the middle domain represented by this model and the C-terminal domain are putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11. | ||
cd10317 | RGL4_C | 9.0e-42 | 403 | 590 | 192 | + C-terminal domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold. Both the middle and the C-terminal domain are putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11. | ||
pfam06045 | Rhamnogal_lyase | 4.0e-58 | 2 | 160 | 159 | + Rhamnogalacturonate lyase family. Rhamnogalacturonate lyase (EC:4.2.2.-) degrades the rhamnogalacturonan I (RG-I) backbone of pectin. This family contains mainly members from plants, but also contains the plant pathogen Erwinia chrysanthemi. | ||
cd10320 | RGL4_N | 4.0e-58 | 3 | 259 | 267 | + N-terminal catalytic domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold; the middle and C-terminal domains are both putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11. |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
EMBL | CBI23231.1 | 0 | 1 | 595 | 26 | 618 | unnamed protein product [Vitis vinifera] |
RefSeq | NP_172460.6 | 0 | 1 | 592 | 25 | 616 | lyase [Arabidopsis thaliana] |
RefSeq | XP_002301112.1 | 0 | 1 | 591 | 25 | 613 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002317123.1 | 0 | 1 | 596 | 41 | 632 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002527357.1 | 0 | 1 | 595 | 41 | 637 | lyase, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3njx_A | 0.0001 | 249 | 570 | 205 | 483 | A Chain A, Rhamnogalacturonan Lyase From Aspergillus Aculeatus Mutant H210a |
PDB | 3njv_A | 0.0002 | 338 | 570 | 283 | 483 | A Chain A, Rhamnogalacturonan Lyase From Aspergillus Aculeatus Mutant H210a |
PDB | 2xhn_B | 0.0002 | 338 | 570 | 283 | 483 | A Chain A, Rhamnogalacturonan Lyase From Aspergillus Aculeatus K150a Active Site Mutant |
PDB | 2xhn_A | 0.0002 | 338 | 570 | 283 | 483 | A Chain A, Rhamnogalacturonan Lyase From Aspergillus Aculeatus K150a Active Site Mutant |
PDB | 1nkg_A | 0.0002 | 338 | 570 | 283 | 483 | A Chain A, Rhamnogalacturonan Lyase From Aspergillus Aculeatus |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
GE473226 | 289 | 146 | 434 | 0 |
GW864372 | 312 | 104 | 415 | 0 |
CO473731 | 279 | 146 | 424 | 0 |
DV995349 | 289 | 146 | 434 | 0 |
CO474992 | 287 | 197 | 483 | 0 |
Sequence Alignments (This image is cropped. Click for full image.) |
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