| Basic Information | |
|---|---|
| Species | Cucumis sativus |
| Cazyme ID | Cucsa.088930.2 |
| Family | GT68 |
| Protein Properties | Length: 496 Molecular Weight: 56140.2 Isoelectric Point: 6.8176 |
| Chromosome | Chromosome/Scaffold: 00894 Start: 145018 End: 148014 |
| Description | O-fucosyltransferase family protein |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
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| Family | Start | End | Evalue |
| GT68 | 177 | 470 | 0 |
| NRIVVIPSSKVDYQYSKVLDVDHVNNCLGRKVVISFEEFSEIQKNQMKIDKFFCYFSKPDHCYLDDEHVKQLNNIGVSTAKLESPWDEDIKNPSKRTVQD IESKFSSDADVIAIGDVFYADVENGWFVQPGGPIAHKCKTLIEPSHLIKVTAQRFIQTFLGSDFIAIHFRRHGFLKFCNAKRPSCFYPIPQAANCIASVV ERSHSPVIYLSTDAADSETGLLQSLVMVNGKPIPLVKRPPRNSAEKWDALLYRHEIEDDSQVEAMLDKAICAMSRVFIGTSGSTFTEDILRLRK | |||
| Full Sequence |
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| Protein Sequence Length: 496 Download |
| MKPITPLHLI PTPSTSALTT IFALPPPVVL SPSKRAFDLL RYRMTESELH ALFLLKQQRL 60 GLLTMWNHSF TSHSNSTSED LKLSLLRQIS LNKEIQNVLL SSHQTGNSSN EVYDDGLDLV 120 GSDFDRCRKV DQEVLNRRTI EWNPKSNKFL FAICASGQMS NHLICLEKHM FFAAILNRIV 180 VIPSSKVDYQ YSKVLDVDHV NNCLGRKVVI SFEEFSEIQK NQMKIDKFFC YFSKPDHCYL 240 DDEHVKQLNN IGVSTAKLES PWDEDIKNPS KRTVQDIESK FSSDADVIAI GDVFYADVEN 300 GWFVQPGGPI AHKCKTLIEP SHLIKVTAQR FIQTFLGSDF IAIHFRRHGF LKFCNAKRPS 360 CFYPIPQAAN CIASVVERSH SPVIYLSTDA ADSETGLLQS LVMVNGKPIP LVKRPPRNSA 420 EKWDALLYRH EIEDDSQVEA MLDKAICAMS RVFIGTSGST FTEDILRLRK DWRSASLCDE 480 YLCQGQDPNF IAENE* |
| Functional Domains Download unfiltered results here | ||||||||
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| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd11296 | O-FucT_like | 0.0001 | 149 | 190 | 42 | + GDP-fucose protein O-fucosyltransferase and related proteins. O-fucosyltransferase-like proteins are GDP-fucose dependent enzymes with similarities to the family 1 glycosyltransferases (GT1). They are soluble ER proteins that may be proteolytically cleaved from a membrane-associated preprotein, and are involved in the O-fucosylation of protein substrates, the core fucosylation of growth factor receptors, and other processes. | ||
| pfam10250 | O-FucT | 5.0e-7 | 172 | 462 | 324 | + GDP-fucose protein O-fucosyltransferase. This is a family of conserved proteins representing the enzyme responsible for adding O-fucose to EGF (epidermal growth factor-like) repeats. Six highly conserved cysteines are present in O-FucT-1 as well as a DXD-like motif (ERD), conserved in mammals, Drosophila, and C. elegans. Both features are characteristic of several glycosyltransferase families. The enzyme is a membrane-bound protein released by proteolysis and, as for most glycosyltransferases, is strongly activated by manganese. | ||
| cd11298 | O-FucT-2 | 2.0e-9 | 336 | 462 | 129 | + GDP-fucose protein O-fucosyltransferase 2. O-FucT-2 adds O-fucose to thrombospondin type 1 repeats (TSRs), and appears conserved in bilateria. The O-fucosylation of TSRs appears to play a role in regulating secretion of metalloproteases of the ADAMTS superfamily. O-fucosyltransferase-like proteins are GDP-fucose dependent enzymes with similarities to the family 1 glycosyltransferases (GT1). They are soluble ER proteins that may be proteolytically cleaved from a membrane-associated preprotein, and are involved in the O-fucosylation of protein substrates, the core fucosylation of growth factor receptors, and other processes. | ||
| cd11296 | O-FucT_like | 6.0e-18 | 311 | 474 | 174 | + GDP-fucose protein O-fucosyltransferase and related proteins. O-fucosyltransferase-like proteins are GDP-fucose dependent enzymes with similarities to the family 1 glycosyltransferases (GT1). They are soluble ER proteins that may be proteolytically cleaved from a membrane-associated preprotein, and are involved in the O-fucosylation of protein substrates, the core fucosylation of growth factor receptors, and other processes. | ||
| Annotations - NR Download unfiltered results here | |||||||
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| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAM66093.1 | 0 | 37 | 495 | 99 | 566 | unknown [Arabidopsis thaliana] |
| RefSeq | NP_173170.2 | 0 | 37 | 495 | 99 | 564 | unknown protein [Arabidopsis thaliana] |
| RefSeq | NP_199853.1 | 0 | 37 | 495 | 99 | 566 | unknown protein [Arabidopsis thaliana] |
| RefSeq | XP_002264087.1 | 0 | 43 | 495 | 87 | 559 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002303337.1 | 0 | 38 | 495 | 64 | 527 | predicted protein [Populus trichocarpa] |
| Annotations - PDB Download unfiltered results here | |||||||
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| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 4ap6_D | 0.00002 | 336 | 462 | 277 | 383 | A Chain A, Crystal Structure Of Human Pofut2 E54a Mutant In Complex With Gdp- Fucose |
| PDB | 4ap6_C | 0.00002 | 336 | 462 | 277 | 383 | A Chain A, Crystal Structure Of Human Pofut2 E54a Mutant In Complex With Gdp- Fucose |
| PDB | 4ap6_B | 0.00002 | 336 | 462 | 277 | 383 | A Chain A, Crystal Structure Of Human Pofut2 E54a Mutant In Complex With Gdp- Fucose |
| PDB | 4ap6_A | 0.00002 | 336 | 462 | 277 | 383 | A Chain A, Crystal Structure Of Human Pofut2 E54a Mutant In Complex With Gdp- Fucose |
| PDB | 4ap5_B | 0.00002 | 336 | 462 | 263 | 369 | A Chain A, Crystal Structure Of Human Pofut2 |
| Hydropathy |
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| EST Download unfiltered results here | ||||
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| Hit | Length | Start | End | EValue |
| GO881872 | 354 | 144 | 494 | 0 |
| ES850268 | 332 | 137 | 466 | 0 |
| CO085239 | 287 | 193 | 478 | 0 |
| EX128555 | 296 | 139 | 433 | 0 |
| CO081771 | 275 | 205 | 478 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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| Phylogeny |
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