| Basic Information | |
|---|---|
| Species | Cucumis sativus |
| Cazyme ID | Cucsa.166320.1 |
| Family | GH32 |
| Protein Properties | Length: 631 Molecular Weight: 69673.1 Isoelectric Point: 4.6927 |
| Chromosome | Chromosome/Scaffold: 01153 Start: 1526537 End: 1532805 |
| Description | Glycosyl hydrolases family 32 protein |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH32 | 111 | 430 | 0 |
| HFQPEGNWMNDPNGPLYHKGWYHLFYQYNPESAVWGNISWGHAVSRDLIHWLYLPYAMVPDQPYDVNGVWTGSATILPDGRIVMLYTGDTIDGVQVQNLA YPANLSDPLLLNWVKHPGNPVLVPPPGIGPKDFRDPTTAWLGPDGKWRITIGSRVGTTLGVSMVYTTDDFIKYELVDRFLHAVPGTGMWECVDFYPVSVD GSKGLDTSENGGGVKHVLKASLDDTKMDHYAIGTYFANNDTWVPDNPEEDVGIGLKLDYGRYYASKTFYDQNKERRILWGWINETDTEANDLAKGWASVQ TVPRTVLFDQKTGSNIIQWP | |||
| Full Sequence |
|---|
| Protein Sequence Length: 631 Download |
| MDSTSSDLIP PPYSALPDRH PSPPLSRKSL NFTTTTILIS FLLIVSLLTL FSYNHPPSPD 60 SAKLPENTQS RLARGVAEGV SAKSNPYFSD SVDGSFNWTN AMFSWQRTAF HFQPEGNWMN 120 DPNGPLYHKG WYHLFYQYNP ESAVWGNISW GHAVSRDLIH WLYLPYAMVP DQPYDVNGVW 180 TGSATILPDG RIVMLYTGDT IDGVQVQNLA YPANLSDPLL LNWVKHPGNP VLVPPPGIGP 240 KDFRDPTTAW LGPDGKWRIT IGSRVGTTLG VSMVYTTDDF IKYELVDRFL HAVPGTGMWE 300 CVDFYPVSVD GSKGLDTSEN GGGVKHVLKA SLDDTKMDHY AIGTYFANND TWVPDNPEED 360 VGIGLKLDYG RYYASKTFYD QNKERRILWG WINETDTEAN DLAKGWASVQ TVPRTVLFDQ 420 KTGSNIIQWP VEEVESLRLG SNEFNDVLLE PGSVVELEVG PATQLDILAE FEVEALGSEN 480 ATVSEEGCGG GAAERSSIGP FGVLVLAHQS LSEFTPIYFN VANSSKGSGE AYFCADETRS 540 SKAPDVFKQV YGSKIPVLEG ENYSMRVLVD HSIVESFGQG GRRVITSRIY PTEAIYGAAK 600 LFLFNNGTSA NVKATVKVWR LNSAFIQPYF * 660 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01322 | scrB_fam | 4.0e-53 | 107 | 591 | 503 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| COG1621 | SacC | 3.0e-71 | 107 | 593 | 507 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 3.0e-96 | 117 | 433 | 328 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 2.0e-153 | 111 | 430 | 327 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 0 | 111 | 581 | 480 | + Glycosyl hydrolases family 32. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004564 | beta-fructofuranosidase activity |
| GO:0004575 | sucrose alpha-glucosidase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAM77272.1 | 0 | 55 | 629 | 84 | 662 | AF519809_1 acid invertase [Lagenaria siceraria] |
| GenBank | ACJ04702.1 | 0 | 1 | 630 | 1 | 630 | invertase 2 [Cucumis melo] |
| DDBJ | BAF34363.1 | 0 | 74 | 629 | 85 | 637 | beta-fructofuranosidase [Citrus sinensis] |
| DDBJ | BAF35858.1 | 0 | 66 | 629 | 80 | 640 | Soluble acid invertase [Pyrus pyrifolia var. culta] |
| DDBJ | BAG30919.1 | 0 | 66 | 629 | 80 | 640 | soluble acid invertase [Pyrus pyrifolia] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3ugh_B | 0 | 96 | 629 | 8 | 544 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| PDB | 3ugh_A | 0 | 96 | 629 | 8 | 544 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| PDB | 3ugg_B | 0 | 96 | 629 | 8 | 544 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| PDB | 3ugg_A | 0 | 96 | 629 | 8 | 544 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| PDB | 3ugf_B | 0 | 96 | 629 | 8 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
