| Basic Information | |
|---|---|
| Species | Cucumis sativus |
| Cazyme ID | Cucsa.176290.3 |
| Family | CBM20 |
| Protein Properties | Length: 447 Molecular Weight: 49356.2 Isoelectric Point: 3.9887 |
| Chromosome | Chromosome/Scaffold: 01225 Start: 603513 End: 606489 |
| Description | Carbohydrate-binding-like fold |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| CBM20 | 95 | 180 | 1.6e-23 |
| VRVKFQLLKECTFGEHFYVVGDDPIFGSWDVTSAIPLNWADGHQWAAEVDIPVGKIIQFKFILQGITGNVVWQPGPDRTFQPWETS | |||
| Full Sequence |
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| Protein Sequence Length: 447 Download |
| MKTLETYNSI IANCSPSSYF SSSSSSLKER LLSGGPEFIS YRRPWKLANS GLQHLVPLRR 60 GGIDFISSCF ASYQQADTIQ NDAVENQETD QSKTVRVKFQ LLKECTFGEH FYVVGDDPIF 120 GSWDVTSAIP LNWADGHQWA AEVDIPVGKI IQFKFILQGI TGNVVWQPGP DRTFQPWETS 180 NTIIVSEDWD SAESRILSEE EKIVNQEEDS PIAPENLMDE DNLTYPDEEL IPNIIKDSIA 240 RKPSVELIDG SNIPALEENG CNISASEENI TNVSLPEGNN SSISDSNDNA KDLVAGNISN 300 KAVSEVYLDD TKITEEILEN DAKDDGVQES PVDDQVPILV PGLPPTATAS NQNAPPHEVE 360 DDGSVCGINE SNDHKLPESQ NIQKNQKLDP EVVAGQEMEA KSSYEDDTNI IENQSDLQEI 420 NNDVVQNDLT WGHKTLKKFL SSLRLL* 480 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd02853 | E_set_MTHase_like_N | 8.0e-15 | 95 | 183 | 90 | + N-terminal Early set domain associated with the catalytic domain of Maltooligosyl trehalose trehalohydrolase (also called Glycosyltrehalose trehalohydrolase) and similar proteins. E or "early" set domains are associated with the catalytic domain of Maltooligosyl trehalose trehalohydrolase (MTHase) and similar proteins at the N-terminal end. This subfamily also includes bacterial alpha amylases and 1,4-alpha-glucan branching enzymes which are highly similar to MTHase. Maltooligosyl trehalose synthase (MTSase) and MTHase work together to produce trehalose. MTSase is responsible for converting the alpha-1,4-glucosidic linkage to an alpha,alpha-1,1-glucosidic linkage at the reducing end of the maltooligosaccharide through an intramolecular transglucosylation reaction, while MTHase hydrolyzes the penultimate alpha-1,4 linkage of the reducing end, resulting in the release of trehalose. The N-terminal domain of MTHase may be related to the immunoglobulin and/or fibronectin type III superfamilies. These domains are associated with different types of catalytic domains at either the N-terminal or C-terminal end and may be involved in homodimeric/tetrameric/dodecameric interactions. Members of this family include members of the alpha amylase family, sialidase, galactose oxidase, cellulase, cellulose, hyaluronate lyase, chitobiase, and chitinase, among others. | ||
| smart01065 | CBM_2 | 2.0e-15 | 95 | 178 | 88 | + Starch binding domain. | ||
| cd02856 | E_set_GDE_Isoamylase_N | 2.0e-16 | 94 | 189 | 101 | + N-terminal Early set domain associated with the catalytic domain of Glycogen debranching enzyme and bacterial isoamylase (also called glycogen 6-glucanohydrolase). E or "early" set domains are associated with the catalytic domain of the glycogen debranching enzyme at the N-terminal end. Glycogen debranching enzymes have both 4-alpha-glucanotransferase and amylo-1,6-glucosidase activities. As a transferase, it transfers a segment of the 1,4-alpha-D-glucan to a new 4-position in an acceptor, which may be glucose or another 1,4-alpha-D-glucan. As a glucosidase, it catalyzes the endohydrolysis of 1,6-alpha-D-glucoside linkages at points of branching in chains of 1,4-linked alpha-D-glucose residues. Bacterial isoamylases are also included in this subfamily. Isoamylase is one of the starch-debranching enzymes that catalyze the hydrolysis of alpha-1,6-glucosidic linkages specific in alpha-glucans such as amylopectin or glycogen. Isoamylase contains a bound calcium ion, but this is not in the same position as the conserved calcium ion that has been reported in other alpha-amylase family enzymes. The N-terminal domain of glycogen debranching enzyme may be related to the immunoglobulin and/or fibronectin type III superfamilies. These domains are associated with different types of catalytic domains at either the N-terminal or C-terminal end and may be involved in homodimeric/tetrameric/dodecameric interactions. Members of this family include members of the alpha amylase family, sialidase, galactose oxidase, cellulase, cellulose, hyaluronate lyase, chitobiase, and chitinase, among others. | ||
| pfam00686 | CBM_20 | 3.0e-18 | 94 | 186 | 96 | + Starch binding domain. | ||
| cd05467 | CBM20 | 5.0e-22 | 96 | 189 | 97 | + The family 20 carbohydrate-binding module (CBM20), also known as the starch-binding domain, is found in a large number of starch degrading enzymes including alpha-amylase, beta-amylase, glucoamylase, and CGTase (cyclodextrin glucanotransferase). CBM20 is also present in proteins that have a regulatory role in starch metabolism in plants (e.g. alpha-amylase) or glycogen metabolism in mammals (e.g. laforin). CBM20 folds as an antiparallel beta-barrel structure with two starch binding sites. These two sites are thought to differ functionally with site 1 acting as the initial starch recognition site and site 2 involved in the specific recognition of appropriate regions of starch. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0005975 | carbohydrate metabolic process |
| GO:2001070 | starch binding |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CAN60108.1 | 0 | 39 | 443 | 5 | 425 | hypothetical protein [Vitis vinifera] |
| RefSeq | NP_974720.1 | 0 | 84 | 446 | 75 | 385 | glycoside hydrolase starch-binding domain-containing protein [Arabidopsis thaliana] |
| RefSeq | XP_002308216.1 | 0 | 89 | 446 | 24 | 421 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002322974.1 | 0 | 81 | 234 | 84 | 237 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002528119.1 | 0 | 89 | 352 | 14 | 291 | catalytic, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2vn7_A | 0.0000002 | 94 | 191 | 496 | 599 | A Chain A, Cyclodextrin Glucanotransferase (E.C.2.4.1.19) (Cgtase) |
| PDB | 2vn4_A | 0.0000002 | 94 | 191 | 496 | 599 | A Chain A, Glycoside Hydrolase Family 15 Glucoamylase From Hypocrea Jecorina |
| PDB | 1cyg_A | 0.0000003 | 83 | 189 | 568 | 678 | A Chain A, Cyclodextrin Glucanotransferase (E.C.2.4.1.19) (Cgtase) |
| PDB | 1kum_A | 0.000005 | 95 | 174 | 7 | 90 | A Chain A, Cyclodextrin Glucanotransferase (E.C.2.4.1.19) (Cgtase) |
| PDB | 1kul_A | 0.000005 | 95 | 174 | 7 | 90 | A Chain A, Cyclodextrin Glucanotransferase (E.C.2.4.1.19) (Cgtase) |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| JG528188 | 235 | 1 | 233 | 0 |
| HS409850 | 287 | 1 | 275 | 0 |
| HS409821 | 254 | 1 | 246 | 0 |
| ES882535 | 141 | 1 | 141 | 0 |
| ES882535 | 52 | 134 | 185 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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