y
Basic Information | |
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Species | Cucumis sativus |
Cazyme ID | Cucsa.201870.1 |
Family | GH18 |
Protein Properties | Length: 296 Molecular Weight: 31129.4 Isoelectric Point: 9.8986 |
Chromosome | Chromosome/Scaffold: 01376 Start: 103819 End: 104706 |
Description | chitinase A |
View CDS |
External Links |
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NCBI Taxonomy |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH18 | 30 | 247 | 1.3e-29 |
IAIYWGQNGNEGSLASTCATGNYKIVNIAFLSTFGSGRTPVLNLAGHCNPNNNNGCAFLSSQIKSCQSRGIKVLLSIGGGAGSYSLSSANDAKQVANFIW NNYLGGRSNSRPFGNAVLNGVDFDIESGSGKFWDVLARELKNKGGVTLSAAPQCPIPDAHLDSAIKTGLFDLVWVQFYNNPSCMYANGNVNNLLNSWNRW TGFPVGKLYMGLPAASAA |
Full Sequence |
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Protein Sequence Length: 296 Download |
MATQKIITTL SIISFLLASI FRSSDAAGGI AIYWGQNGNE GSLASTCATG NYKIVNIAFL 60 STFGSGRTPV LNLAGHCNPN NNNGCAFLSS QIKSCQSRGI KVLLSIGGGA GSYSLSSAND 120 AKQVANFIWN NYLGGRSNSR PFGNAVLNGV DFDIESGSGK FWDVLARELK NKGGVTLSAA 180 PQCPIPDAHL DSAIKTGLFD LVWVQFYNNP SCMYANGNVN NLLNSWNRWT GFPVGKLYMG 240 LPAASAAAPS GGFIPANVLK SKVLPRIKSS SKYGGIMLWS KAFDNGYSNA IKGSL* 300 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
cd00598 | GH18_chitinase-like | 0.0007 | 30 | 208 | 196 | + The GH18 (glycosyl hydrolase, family 18) type II chitinases hydrolyze chitin, an abundant polymer of beta-1,4-linked N-acetylglucosamine (GlcNAc) which is a major component of the cell wall of fungi and the exoskeleton of arthropods. Chitinases have been identified in viruses, bacteria, fungi, protozoan parasites, insects, and plants. The structure of the GH18 domain is an eight-stranded beta/alpha barrel with a pronounced active-site cleft at the C-terminal end of the beta-barrel. The GH18 family includes chitotriosidase, chitobiase, hevamine, zymocin-alpha, narbonin, SI-CLP (stabilin-1 interacting chitinase-like protein), IDGF (imaginal disc growth factor), CFLE (cortical fragment-lytic enzyme) spore hydrolase, the type III and type V plant chitinases, the endo-beta-N-acetylglucosaminidases, and the chitolectins. The GH85 (glycosyl hydrolase, family 85) ENGases (endo-beta-N-acetylglucosaminidases) are closely related to the GH18 chitinases and are included in this alignment model. | ||
pfam00704 | Glyco_hydro_18 | 7.0e-18 | 28 | 207 | 202 | + Glycosyl hydrolases family 18. | ||
cd02877 | GH18_hevamine_XipI_class_III | 3.0e-104 | 28 | 293 | 281 | + This conserved domain family includes xylanase inhibitor Xip-I, and the class III plant chitinases such as hevamine, concanavalin B, and PPL2, all of which have a glycosyl hydrolase family 18 (GH18) domain. Hevamine is a class III endochitinase that hydrolyzes the linear polysaccharide chains of chitin and peptidoglycan and is important for defense against pathogenic bacteria and fungi. PPL2 (Parkia platycephala lectin 2) is a class III chitinase from Parkia platycephala seeds that hydrolyzes beta(1-4) glycosidic bonds linking 2-acetoamido-2-deoxy-beta-D-glucopyranose units in chitin. |
Gene Ontology | |
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GO Term | Description |
GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
GO:0005975 | carbohydrate metabolic process |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | AAC37394.1 | 0 | 1 | 295 | 1 | 292 | ORF 1 [Cucumis sativus] |
GenBank | AAF64474.1 | 0 | 1 | 295 | 1 | 291 | AF241266_1 chitinase 1 [Cucumis melo] |
GenBank | AAL01886.1 | 0 | 1 | 295 | 1 | 292 | AF404590_1 chitinase 3-like protein precursor [Trichosanthes kirilowii] |
GenBank | ABA26457.1 | 0 | 1 | 295 | 1 | 291 | acidic class III chitinase [Citrullus lanatus] |
Swiss-Prot | P17541 | 0 | 1 | 295 | 1 | 291 | CHIA_CUCSA RecName: Full=Acidic endochitinase; Flags: Precursor |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 2gsj_A | 0 | 28 | 295 | 1 | 271 | A Chain A, Cdna Cloning And 1.75a Crystal Structure Determination Of Ppl2, A Novel Chimerolectin From Parkia Platycephala Seeds Exhibiting Endochitinolytic Activity |
PDB | 2hvm_A | 0 | 28 | 295 | 1 | 273 | A Chain A, Cdna Cloning And 1.75a Crystal Structure Determination Of Ppl2, A Novel Chimerolectin From Parkia Platycephala Seeds Exhibiting Endochitinolytic Activity |
PDB | 1llo_A | 0 | 28 | 295 | 1 | 273 | A Chain A, Cdna Cloning And 1.75a Crystal Structure Determination Of Ppl2, A Novel Chimerolectin From Parkia Platycephala Seeds Exhibiting Endochitinolytic Activity |
PDB | 1hvq_A | 0 | 28 | 295 | 1 | 273 | A Chain A, Crystal Structures Of Hevamine, A Plant Defence Protein With Chitinase And Lysozyme Activity, And Its Complex With An Inhibitor |
PDB | 1kr0_A | 0 | 28 | 295 | 1 | 273 | A Chain A, Hevamine Mutant D125aY183F IN COMPLEX WITH TETRA-Nag |