y
Basic Information | |
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Species | Cucumis sativus |
Cazyme ID | Cucsa.228080.1 |
Family | GH31 |
Protein Properties | Length: 918 Molecular Weight: 104080 Isoelectric Point: 6.2247 |
Chromosome | Chromosome/Scaffold: 01933 Start: 74337 End: 82014 |
Description | Glycosyl hydrolases family 31 protein |
View CDS |
External Links |
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NCBI Taxonomy |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH31 | 332 | 776 | 0 |
FFFVGPGPKDVVRQYTSVTGTSAMPQLFATAYHQCRWNYRDEEDVAHVDSKFDEHDIPYDVLWLDIDHTDGKRYMTWDRSLFPNPEEMQKKLAAKGRYMV TVVDPHVKREDSFTLHKEASKKGYYVKDAAGNDYDGWCWPGSSSYLDALSPEVRSWWGEKFSLQNYVGSTPTLYIWNDMNEPSVFSGPEGTMPRNALHYG GVEHRELHNAYGYYFHMATSEGLVKRGDGNDRPFVLSRAAFAGTQKYGTVWTGDSSAEWDYLRVSVPMVLTLGLTGLSFSGADVGGFFGNPEAELLVRWF QLGAFYPFFRGHAHHDTKRREPWLFGERNTELMRDAIRVRYVLLPYFYTLFREANMTGIPVVRPLWMEFPSDEVTFKNDEAFMVGSALLVQGIYTKEAKK VSVYLPGKQSWYDFRTGTIYKGGITHQLEVFEESIPTFQKAGTII |
Full Sequence |
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Protein Sequence Length: 918 Download |
MRAPYLLLLL LLSLHLTLVL PWKKDEFRNC NQTPFCKRAR AFKRGSCSLV AHDVSINDGD 60 LTAKLLPRNQ DPDHPPNPLL LVLSVYQDGI VRLRIDEDPS LGPPKKRFQL PNVIVDEFLS 120 QKLWLQRIST ETIGSDLRPS SIVYLSDGYE AVLRQDPFEV FVREKSGKRV LSLNSHGLFD 180 FEQLRVKDEG EDWEEKFRGH TDTRPFGPQS ISFDVSFYDA DFVYGIPEHA TSLALKPTRG 240 PDVEESEPYR LFNLDVFEYL HDSPFGLYGS IPFMISHGKS RGTSGFFWLN AAEMQIDVLG 300 SGWDAESGIS LPSSQSSIDT FWMSEAGIVD TFFFVGPGPK DVVRQYTSVT GTSAMPQLFA 360 TAYHQCRWNY RDEEDVAHVD SKFDEHDIPY DVLWLDIDHT DGKRYMTWDR SLFPNPEEMQ 420 KKLAAKGRYM VTVVDPHVKR EDSFTLHKEA SKKGYYVKDA AGNDYDGWCW PGSSSYLDAL 480 SPEVRSWWGE KFSLQNYVGS TPTLYIWNDM NEPSVFSGPE GTMPRNALHY GGVEHRELHN 540 AYGYYFHMAT SEGLVKRGDG NDRPFVLSRA AFAGTQKYGT VWTGDSSAEW DYLRVSVPMV 600 LTLGLTGLSF SGADVGGFFG NPEAELLVRW FQLGAFYPFF RGHAHHDTKR REPWLFGERN 660 TELMRDAIRV RYVLLPYFYT LFREANMTGI PVVRPLWMEF PSDEVTFKND EAFMVGSALL 720 VQGIYTKEAK KVSVYLPGKQ SWYDFRTGTI YKGGITHQLE VFEESIPTFQ KAGTIIPRKD 780 RSRRSSTQMV NDPYTLVVAL NSSQAAEGEL YIDDGKSFEF KQGAFIHRRF VFSDGKLTSL 840 NVGPIASSST KFSSNCVIER IILLGHSGAK SALVEPENRK VDIELGPLHF LRGRGSSVLT 900 IRKPNLLISD DWTVKVV* 960 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
cd06600 | GH31_MGAM-like | 9.0e-125 | 351 | 689 | 339 | + This family includes the following closely related glycosyl hydrolase family 31 (GH31) enzymes: maltase-glucoamylase (MGAM), sucrase-isomaltase (SI), lysosomal acid alpha-glucosidase (GAA), neutral alpha-glucosidase C (GANC), the alpha subunit of neutral alpha-glucosidase AB (GANAB), and alpha-glucosidase II. MGAM is one of the two enzymes responsible for catalyzing the last glucose-releasing step in starch digestion. SI is implicated in the digestion of dietary starch and major disaccharides such as sucrose and isomaltose, while GAA degrades glycogen in the lysosome, cleaving both alpha-1,4 and alpha-1,6 glucosidic linkages. MGAM and SI are anchored to small-intestinal brush-border epithelial cells. The absence of SI from the brush border membrane or its malfunction is associated with malabsorption disorders such as congenital sucrase-isomaltase deficiency (CSID). The domain architectures of MGAM and SI include two tandem GH31 catalytic domains, an N-terminal domain found near the membrane-bound end and a C-terminal luminal domain. Both of the tandem GH31 domains of MGAM and SI are included in this family. The domain architecture of GAA includes an N-terminal TFF (trefoil factor family) domain in addition to the GH31 catalytic domain. Deficient GAA expression causes pompe disease, an autosomal recessive genetic disorder also known as glycogen storage disease type II (GSDII). GANC and GANAB are key enzymes in glycogen metabolism that hydrolyze terminal, non-reducing 1,4-linked alpha-D-glucose residues from glycogen in the endoplasmic reticulum. Alpha-glucosidase II is a GH31 enzyme, found in bacteria and plants, which has exo-alpha-1,4-glucosidase and oligo-1,6-glucosidase activities. Alpha-glucosidase II has been characterized in Bacillus thermoamyloliquefaciens where it forms a homohexamer. This family also includes the MalA alpha-glucosidase from Sulfolobus sulfataricus and the AglA alpha-glucosidase from Picrophilus torridus. MalA is part of the carbohydrate-metabolizing machinery that allows this organism to utilize carbohydrates, such as maltose, as the sole carbon and energy source. The MGAM-like family corresponds to subgroup 1 in the Ernst et al classification of GH31 enzymes. | ||
cd06604 | GH31_glucosidase_II_MalA | 5.0e-144 | 351 | 689 | 346 | + Alpha-glucosidase II (alpha-D-glucoside glucohydrolase) is a glycosyl hydrolase family 31 (GH31) enzyme, found in bacteria and plants, which has exo-alpha-1,4-glucosidase and oligo-1,6-glucosidase activities. Alpha-glucosidase II has been characterized in Bacillus thermoamyloliquefaciens where it forms a homohexamer. This family also includes the MalA alpha-glucosidase from Sulfolobus sulfataricus and the AglA alpha-glucosidase from Picrophilus torridus. MalA is part of the carbohydrate-metabolizing machinery that allows this organism to utilize carbohydrates, such as maltose, as the sole carbon and energy source. | ||
COG1501 | COG1501 | 3.0e-174 | 193 | 839 | 651 | + Alpha-glucosidases, family 31 of glycosyl hydrolases [Carbohydrate transport and metabolism] | ||
cd06603 | GH31_GANC_GANAB_alpha | 0 | 351 | 689 | 339 | + This family includes the closely related glycosyl hydrolase family 31 (GH31) isozymes, neutral alpha-glucosidase C (GANC) and the alpha subunit of heterodimeric neutral alpha-glucosidase AB (GANAB). Initially distinguished on the basis of differences in electrophoretic mobility in starch gel, GANC and GANAB have been shown to have other differences, including those of substrate specificity. GANC and GANAB are key enzymes in glycogen metabolism that hydrolyze terminal, non-reducing 1,4-linked alpha-D-glucose residues from glycogen in the endoplasmic reticulum. The GANC/GANAB family includes the alpha-glucosidase II (ModA) from Dictyostelium discoideum as well as the alpha-glucosidase II (GLS2, or ROT2 - Reversal of TOR2 lethality protein 2) from Saccharomyces cerevisiae. | ||
pfam01055 | Glyco_hydro_31 | 0 | 332 | 776 | 450 | + Glycosyl hydrolases family 31. Glycosyl hydrolases are key enzymes of carbohydrate metabolism. Family 31 comprises of enzymes that are, or similar to, alpha- galactosidases. |
Gene Ontology | |
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GO Term | Description |
GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
GO:0005975 | carbohydrate metabolic process |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
RefSeq | NP_201189.1 | 0 | 6 | 917 | 5 | 921 | RSW3 (RADIAL SWELLING 3); glucosidase/ hydrolase, hydrolyzing O-glycosyl compounds [Arabidopsis thaliana] |
RefSeq | XP_002268690.1 | 0 | 19 | 917 | 25 | 926 | PREDICTED: hypothetical protein [Vitis vinifera] |
RefSeq | XP_002270200.1 | 0 | 19 | 917 | 25 | 926 | PREDICTED: hypothetical protein [Vitis vinifera] |
RefSeq | XP_002310537.1 | 0 | 3 | 917 | 5 | 932 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002529411.1 | 0 | 5 | 917 | 6 | 923 | neutral alpha-glucosidase ab precursor, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3w38_A | 0 | 223 | 865 | 207 | 850 | A Chain A, Characterization And Engineering Of The Bifunctional N- And O-glucosyltransferase Involved In Xenobiotic Metabolism In Plants |
PDB | 3w37_A | 0 | 223 | 865 | 207 | 850 | A Chain A, Characterization And Engineering Of The Bifunctional N- And O-glucosyltransferase Involved In Xenobiotic Metabolism In Plants |
PDB | 3l4z_A | 0 | 266 | 875 | 212 | 830 | A Chain A, Characterization And Engineering Of The Bifunctional N- And O-glucosyltransferase Involved In Xenobiotic Metabolism In Plants |
PDB | 3l4y_A | 0 | 266 | 875 | 212 | 830 | A Chain A, Characterization And Engineering Of The Bifunctional N- And O-glucosyltransferase Involved In Xenobiotic Metabolism In Plants |
PDB | 3l4x_A | 0 | 266 | 875 | 212 | 830 | A Chain A, Characterization And Engineering Of The Bifunctional N- And O-glucosyltransferase Involved In Xenobiotic Metabolism In Plants |