y
Basic Information | |
---|---|
Species | Cucumis sativus |
Cazyme ID | Cucsa.229010.2 |
Family | GH35 |
Protein Properties | Length: 712 Molecular Weight: 78734.4 Isoelectric Point: 7.7379 |
Chromosome | Chromosome/Scaffold: 01938 Start: 267 End: 6508 |
Description | beta galactosidase 1 |
View CDS |
External Links |
---|
NCBI Taxonomy |
CAZyDB |
Signature Domain Download full data set without filtering | |||
---|---|---|---|
Family | Start | End | Evalue |
GH35 | 8 | 206 | 0 |
GISFRTDNAPFKFQMERFTRKIVNMMKAERLYESQGGPIILSQIENEYGPMEYELGAPGKAYSKWAAQMALGLGTGVPWVMCKQDDAPDPIINTCNGFYC DYFSPNKAYKPKMWTEAWTGWFTQFGGAVPHRPAEDMAFAVARFIQKGGALINYYMYHGGTNFGRTAGGPFIATSYDYDAPIDEYGLLRQPKWGHLKDL |
Full Sequence |
---|
Protein Sequence Length: 712 Download |
MELWYIPGIS FRTDNAPFKF QMERFTRKIV NMMKAERLYE SQGGPIILSQ IENEYGPMEY 60 ELGAPGKAYS KWAAQMALGL GTGVPWVMCK QDDAPDPIIN TCNGFYCDYF SPNKAYKPKM 120 WTEAWTGWFT QFGGAVPHRP AEDMAFAVAR FIQKGGALIN YYMYHGGTNF GRTAGGPFIA 180 TSYDYDAPID EYGLLRQPKW GHLKDLNRAI KLCEPALVSG DPIVTRLGNY QEAHVFKSKS 240 GACAAFLSNY NPRSYATVAF GNMHYNIPPW SISILPDCKN TVFNTARVGA QTAIMKMSPV 300 PMHESFSWQA YNEEPASYNE KAFTTVGLLE QINTTRDATD YLWYTTDVHI DANEGFLRSG 360 KYPVLTVLSA GHAMHVFVNG QLAGTAYGSL DFPKLTFSRG VNLRAGNNKI ALLSIAVGLP 420 NVGPHFEMWN AGILGPVNLN GLDEGRRDLT WQKWTYKIGL DGEAMSLHSL SGSSSVEWIQ 480 GSLVAQKQPL TWFKTTFNAP AGNSPLALDM GSMGKGQIWL NGQSLGRYWP AYKSTGSCGS 540 CDYTGTYNEK KCSSNCGEAS QRWYHVPRSW LNPTGNLLVV FEEWGGDPNG IHLVRRDVDS 600 VCVNINEWQP TLMNWQMQSS GKVNKPLRPK AHLSCGPGQK ISSVKFASFG TPEGECGSFR 660 EGSCHAHHSY DAFQRTCVGQ NFCTVTVAPE MFGGDPCPNV MKKLSVEVIC S* 720 |
Functional Domains Download unfiltered results here | ||||||||
---|---|---|---|---|---|---|---|---|
Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
cd04082 | CBM35_pectate_lyase-like | 0.0008 | 373 | 422 | 58 | + Carbohydrate Binding Module family 35 (CBM35), pectate lyase-like; appended mainly to enzymes that bind mannan (Man), xylan, glucuronic acid (GlcA) and possibly glucans. This family includes carbohydrate binding module family 35 (CBM35) domains that are non-catalytic carbohydrate binding domains that are appended mainly to enzymes that bind mannan (Man), xylan, glucuronic acid (GlcA) and possibly glucans. Included in this family are pectate lyases, including pectate lyase 10A from Cellvibrio japonicas, these enzymes release delta-4,5-anhydrogalaturonic acid (delta4,5-GalA) from pectin, thus identifying a signature molecule for plant cell wall degradation. CBM35s are unique in that they display conserved specificity through extensive sequence similarity but divergent function through their appended catalytic modules. They are known to bind alpha-D-galactose (Gal), mannan (Man), xylan, glucuronic acid (GlcA), a beta-polymer of mannose, and possibly glucans, forming four subfamilies based on general ligand specificities (galacto, urono, manno, and gluco configurations). In contrast to most CBMs that are generally rigid proteins, CBM35 undergoes significant conformational change upon ligand binding. Some CBM35s bind their ligands in a calcium-dependent manner, especially those binding uronic acids. | ||
pfam02140 | Gal_Lectin | 6.0e-21 | 633 | 710 | 82 | + Galactose binding lectin domain. | ||
pfam01301 | Glyco_hydro_35 | 2.0e-85 | 3 | 210 | 224 | + Glycosyl hydrolases family 35. | ||
PLN03059 | PLN03059 | 0 | 3 | 711 | 710 | + beta-galactosidase; Provisional |
Gene Ontology | |
---|---|
GO Term | Description |
GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
GO:0005529 | Interacting selectively and non-covalently with any carbohydrate, which includes monosaccharides, oligosaccharides and polysaccharides as well as substances derived from monosaccharides by reduction of the carbonyl group (alditols), by oxidation of one or more hydroxy groups to afford the corresponding aldehydes, ketones, or carboxylic acids, or by replacement of one or more hydroxy group(s) by a hydrogen atom. Cyclitols are generally not regarded as carbohydrates." [CHEBI:16646, GOC:mah] |
GO:0005975 | carbohydrate metabolic process |
Annotations - NR Download unfiltered results here | |||||||
---|---|---|---|---|---|---|---|
Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | ABK96254.1 | 0 | 3 | 711 | 138 | 846 | unknown [Populus trichocarpa x Populus deltoides] |
GenBank | ABV32545.1 | 0 | 3 | 711 | 133 | 841 | beta-galactosidase protein 2 [Prunus persica] |
EMBL | CAC44500.1 | 0 | 3 | 711 | 135 | 843 | beta-galactosidase [Fragaria x ananassa] |
RefSeq | XP_002327432.1 | 0 | 3 | 711 | 131 | 839 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002527409.1 | 0 | 3 | 711 | 137 | 845 | beta-galactosidase, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
---|---|---|---|---|---|---|---|
Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3d3a_A | 0.00000000000003 | 9 | 206 | 119 | 325 | A Chain A, Crystal Structure Of A Beta-Galactosidase From Bacteroides Thetaiotaomicron |
PDB | 4e8d_B | 0.000000001 | 43 | 193 | 145 | 313 | A Chain A, Crystal Structure Of A Beta-Galactosidase From Bacteroides Thetaiotaomicron |
PDB | 4e8d_B | 0.0002 | 341 | 596 | 385 | 584 | A Chain A, Crystal Structure Of A Beta-Galactosidase From Bacteroides Thetaiotaomicron |
PDB | 4e8d_A | 0.000000001 | 43 | 193 | 145 | 313 | A Chain A, Crystal Structure Of A Beta-Galactosidase From Bacteroides Thetaiotaomicron |
EST Download unfiltered results here | ||||
---|---|---|---|---|
Hit | Length | Start | End | EValue |
HO780602 | 607 | 3 | 596 | 0 |
HO778160 | 600 | 3 | 600 | 0 |
HO795552 | 511 | 3 | 512 | 0 |
HO784793 | 321 | 34 | 354 | 0 |
GW868956 | 316 | 292 | 607 | 0 |
Sequence Alignments (This image is cropped. Click for full image.) |
---|