y
Basic Information | |
---|---|
Species | Cucumis sativus |
Cazyme ID | Cucsa.229010.3 |
Family | GH35 |
Protein Properties | Length: 694 Molecular Weight: 77314 Isoelectric Point: 7.8651 |
Chromosome | Chromosome/Scaffold: 01938 Start: 267 End: 6508 |
Description | beta galactosidase 1 |
View CDS |
External Links |
---|
NCBI Taxonomy |
CAZyDB |
Signature Domain Download full data set without filtering | |||
---|---|---|---|
Family | Start | End | Evalue |
GH35 | 36 | 336 | 0 |
IINGHRRILISGSIHYPRSTSEMWPDLIQKAKEGGLDVIETYVFWNGHEPEPGKYYFEGNYDLVRFVKLVHQAGLYVHLRIGPYVCAEWNFGGFPVWLKY IPGISFRTDNAPFKFQMERFTRKIVNMMKAERLYESQGGPIILSQIENEYGPMEYELGAPGKAYSKWAAQMALGLGTGVPWVMCKQDDAPDPIINTCNGF YCDYFSPNKAYKPKMWTEAWTGWFTQFGGAVPHRPAEDMAFAVARFIQKGGALINYYMYHGGTNFGRTAGGPFIATSYDYDAPIDEYGLLRQPKWGHLKD L |
Full Sequence |
---|
Protein Sequence Length: 694 Download |
MELENLKMWN VIMGFLCFFG VLSVQASVSY DSKAIIINGH RRILISGSIH YPRSTSEMWP 60 DLIQKAKEGG LDVIETYVFW NGHEPEPGKY YFEGNYDLVR FVKLVHQAGL YVHLRIGPYV 120 CAEWNFGGFP VWLKYIPGIS FRTDNAPFKF QMERFTRKIV NMMKAERLYE SQGGPIILSQ 180 IENEYGPMEY ELGAPGKAYS KWAAQMALGL GTGVPWVMCK QDDAPDPIIN TCNGFYCDYF 240 SPNKAYKPKM WTEAWTGWFT QFGGAVPHRP AEDMAFAVAR FIQKGGALIN YYMYHGGTNF 300 GRTAGGPFIA TSYDYDAPID EYGLLRQPKW GHLKDLNRAI KLCEPALVSG DPIVTRLGNY 360 QEAHVFKSKS GACAAFLSNY NPRSYATVAF GNMHYNIPPW SISILPDCKN TVFNTARVGA 420 QTAIMKMSPV PMHESFSWQA YNEEPASYNE KAFTTVGLLE QINTTRDATD YLWYTTDVHI 480 DANEGFLRSG KYPVLTVLSA GHAMHVFVNG QLAGTAYGSL DFPKLTFSRG VNLRAGNNKI 540 ALLSIAVGLP NVGPHFEMWN AGILGPVNLN GLDEGRRDLT WQKWTYKIGL DGEAMSLHSL 600 SGSSSVEWIQ GSLVAQKQPL TWFKTTFNAP AGNSPLALDM GSMGKGQIWL NGQSLGRYWP 660 AYKSTGSCGS CDYTGTYNEK KCSSNCGEAS QRW* 720 |
Functional Domains Download unfiltered results here | ||||||||
---|---|---|---|---|---|---|---|---|
Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
cd04082 | CBM35_pectate_lyase-like | 0.0008 | 503 | 552 | 58 | + Carbohydrate Binding Module family 35 (CBM35), pectate lyase-like; appended mainly to enzymes that bind mannan (Man), xylan, glucuronic acid (GlcA) and possibly glucans. This family includes carbohydrate binding module family 35 (CBM35) domains that are non-catalytic carbohydrate binding domains that are appended mainly to enzymes that bind mannan (Man), xylan, glucuronic acid (GlcA) and possibly glucans. Included in this family are pectate lyases, including pectate lyase 10A from Cellvibrio japonicas, these enzymes release delta-4,5-anhydrogalaturonic acid (delta4,5-GalA) from pectin, thus identifying a signature molecule for plant cell wall degradation. CBM35s are unique in that they display conserved specificity through extensive sequence similarity but divergent function through their appended catalytic modules. They are known to bind alpha-D-galactose (Gal), mannan (Man), xylan, glucuronic acid (GlcA), a beta-polymer of mannose, and possibly glucans, forming four subfamilies based on general ligand specificities (galacto, urono, manno, and gluco configurations). In contrast to most CBMs that are generally rigid proteins, CBM35 undergoes significant conformational change upon ligand binding. Some CBM35s bind their ligands in a calcium-dependent manner, especially those binding uronic acids. | ||
pfam02449 | Glyco_hydro_42 | 5.0e-7 | 50 | 186 | 152 | + Beta-galactosidase. This group of beta-galactosidase enzymes belong to the glycosyl hydrolase 42 family. The enzyme catalyzes the hydrolysis of terminal, non-reducing terminal beta-D-galactosidase residues. | ||
COG1874 | LacA | 8.0e-25 | 28 | 330 | 365 | + Beta-galactosidase [Carbohydrate transport and metabolism] | ||
pfam01301 | Glyco_hydro_35 | 1.0e-161 | 34 | 340 | 323 | + Glycosyl hydrolases family 35. | ||
PLN03059 | PLN03059 | 0 | 21 | 693 | 674 | + beta-galactosidase; Provisional |
Gene Ontology | |
---|---|
GO Term | Description |
GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
GO:0005975 | carbohydrate metabolic process |
Annotations - NR Download unfiltered results here | |||||||
---|---|---|---|---|---|---|---|
Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | ABK96254.1 | 0 | 23 | 693 | 28 | 698 | unknown [Populus trichocarpa x Populus deltoides] |
GenBank | ABV32545.1 | 0 | 21 | 693 | 21 | 693 | beta-galactosidase protein 2 [Prunus persica] |
EMBL | CAC44500.1 | 0 | 17 | 693 | 22 | 695 | beta-galactosidase [Fragaria x ananassa] |
RefSeq | XP_002327432.1 | 0 | 23 | 693 | 21 | 691 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002527409.1 | 0 | 3 | 693 | 6 | 697 | beta-galactosidase, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
---|---|---|---|---|---|---|---|
Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3d3a_A | 1.4013e-45 | 25 | 336 | 5 | 325 | A Chain A, Crystal Structure Of A Beta-Galactosidase From Bacteroides Thetaiotaomicron |
PDB | 3thd_D | 9.99995e-41 | 28 | 410 | 11 | 405 | A Chain A, Crystal Structure Of A Beta-Galactosidase From Bacteroides Thetaiotaomicron |
PDB | 3thd_C | 9.99995e-41 | 28 | 410 | 11 | 405 | A Chain A, Crystal Structure Of A Beta-Galactosidase From Bacteroides Thetaiotaomicron |
EST Download unfiltered results here | ||||
---|---|---|---|---|
Hit | Length | Start | End | EValue |
HO778160 | 686 | 11 | 693 | 0 |
HO780602 | 606 | 78 | 682 | 0 |
HO795552 | 526 | 118 | 642 | 0 |
HO795552 | 65 | 15 | 79 | 3e-38 |
HO795552 | 51 | 79 | 129 | 3e-38 |
Sequence Alignments (This image is cropped. Click for full image.) |
---|