Basic Information | |
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Species | Cucumis sativus |
Cazyme ID | Cucsa.229010.4 |
Family | GH35 |
Protein Properties | Length: 593 Molecular Weight: 65144.7 Isoelectric Point: 7.8063 |
Chromosome | Chromosome/Scaffold: 01938 Start: 267 End: 4581 |
Description | beta galactosidase 1 |
View CDS |
External Links |
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NCBI Taxonomy |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH35 | 1 | 87 | 7.3e-37 |
MWTEAWTGWFTQFGGAVPHRPAEDMAFAVARFIQKGGALINYYMYHGGTNFGRTAGGPFIATSYDYDAPIDEYGLLRQPKWGHLKDL |
Full Sequence |
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Protein Sequence Length: 593 Download |
MWTEAWTGWF TQFGGAVPHR PAEDMAFAVA RFIQKGGALI NYYMYHGGTN FGRTAGGPFI 60 ATSYDYDAPI DEYGLLRQPK WGHLKDLNRA IKLCEPALVS GDPIVTRLGN YQEAHVFKSK 120 SGACAAFLSN YNPRSYATVA FGNMHYNIPP WSISILPDCK NTVFNTARVG AQTAIMKMSP 180 VPMHESFSWQ AYNEEPASYN EKAFTTVGLL EQINTTRDAT DYLWYTTDVH IDANEGFLRS 240 GKYPVLTVLS AGHAMHVFVN GQLAGTAYGS LDFPKLTFSR GVNLRAGNNK IALLSIAVGL 300 PNVGPHFEMW NAGILGPVNL NGLDEGRRDL TWQKWTYKIG LDGEAMSLHS LSGSSSVEWI 360 QGSLVAQKQP LTWFKTTFNA PAGNSPLALD MGSMGKGQIW LNGQSLGRYW PAYKSTGSCG 420 SCDYTGTYNE KKCSSNCGEA SQRWYHVPRS WLNPTGNLLV VFEEWGGDPN GIHLVRRDVD 480 SVCVNINEWQ PTLMNWQMQS SGKVNKPLRP KAHLSCGPGQ KISSVKFASF GTPEGECGSF 540 REGSCHAHHS YDAFQRTCVG QNFCTVTVAP EMFGGDPCPN VMKKLSVEVI CS* 600 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
cd04082 | CBM35_pectate_lyase-like | 0.001 | 254 | 303 | 58 | + Carbohydrate Binding Module family 35 (CBM35), pectate lyase-like; appended mainly to enzymes that bind mannan (Man), xylan, glucuronic acid (GlcA) and possibly glucans. This family includes carbohydrate binding module family 35 (CBM35) domains that are non-catalytic carbohydrate binding domains that are appended mainly to enzymes that bind mannan (Man), xylan, glucuronic acid (GlcA) and possibly glucans. Included in this family are pectate lyases, including pectate lyase 10A from Cellvibrio japonicas, these enzymes release delta-4,5-anhydrogalaturonic acid (delta4,5-GalA) from pectin, thus identifying a signature molecule for plant cell wall degradation. CBM35s are unique in that they display conserved specificity through extensive sequence similarity but divergent function through their appended catalytic modules. They are known to bind alpha-D-galactose (Gal), mannan (Man), xylan, glucuronic acid (GlcA), a beta-polymer of mannose, and possibly glucans, forming four subfamilies based on general ligand specificities (galacto, urono, manno, and gluco configurations). In contrast to most CBMs that are generally rigid proteins, CBM35 undergoes significant conformational change upon ligand binding. Some CBM35s bind their ligands in a calcium-dependent manner, especially those binding uronic acids. | ||
pfam02140 | Gal_Lectin | 5.0e-21 | 514 | 591 | 82 | + Galactose binding lectin domain. | ||
pfam01301 | Glyco_hydro_35 | 6.0e-41 | 1 | 91 | 94 | + Glycosyl hydrolases family 35. | ||
PLN03059 | PLN03059 | 0 | 1 | 592 | 593 | + beta-galactosidase; Provisional |
Gene Ontology | |
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GO Term | Description |
GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
GO:0005529 | Interacting selectively and non-covalently with any carbohydrate, which includes monosaccharides, oligosaccharides and polysaccharides as well as substances derived from monosaccharides by reduction of the carbonyl group (alditols), by oxidation of one or more hydroxy groups to afford the corresponding aldehydes, ketones, or carboxylic acids, or by replacement of one or more hydroxy group(s) by a hydrogen atom. Cyclitols are generally not regarded as carbohydrates." [CHEBI:16646, GOC:mah] |
GO:0005975 | carbohydrate metabolic process |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | ABK96254.1 | 0 | 1 | 592 | 255 | 846 | unknown [Populus trichocarpa x Populus deltoides] |
GenBank | ABV32545.1 | 0 | 1 | 592 | 250 | 841 | beta-galactosidase protein 2 [Prunus persica] |
EMBL | CAC44500.1 | 0 | 1 | 592 | 252 | 843 | beta-galactosidase [Fragaria x ananassa] |
RefSeq | XP_002327432.1 | 0 | 1 | 592 | 248 | 839 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002329051.1 | 0 | 1 | 592 | 239 | 830 | predicted protein [Populus trichocarpa] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3d3a_A | 0.00000002 | 1 | 87 | 236 | 325 | A Chain A, Crystal Structure Of A Beta-Galactosidase From Bacteroides Thetaiotaomicron |
PDB | 4e8d_B | 0.0000001 | 1 | 74 | 235 | 313 | A Chain A, Crystal Structure Of A Beta-Galactosidase From Bacteroides Thetaiotaomicron |
PDB | 4e8d_B | 0.0001 | 222 | 477 | 385 | 584 | A Chain A, Crystal Structure Of A Beta-Galactosidase From Bacteroides Thetaiotaomicron |
PDB | 4e8d_A | 0.0000001 | 1 | 74 | 235 | 313 | A Chain A, Crystal Structure Of A Beta-Galactosidase From Bacteroides Thetaiotaomicron |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
HO780602 | 490 | 1 | 477 | 0 |
HO778160 | 483 | 1 | 481 | 0 |
HO795552 | 394 | 1 | 393 | 0 |
GW868956 | 316 | 173 | 488 | 0 |
EC935081 | 292 | 302 | 593 | 0 |
Sequence Alignments (This image is cropped. Click for full image.) |
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